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公开(公告)号：KR101552595B1
公开(公告)日：2015-09-14
申请号：KR1020120004022
申请日：2012-01-12
Applicant: 서울대학교산학협력단
Abstract: 본발명은피기백전위를이용한소 형질전환배아의제조방법에관한것이다. 본발명의신규한유전자전달시스템인피기백전위를적용하여 ubiquitous 유전자발현또는목적유전자 on/off 조절이가능한소 형질전환배아를제조할경우, 모든배아에서균일하게목적유전자를발현시킬수 있으며, 나아가항생제조절에의해목적유전자의발현을조절할수 있는안정적인소 형질전환배아를제조할수 있어여러배아관련분야의연구에서유용하게이용될수 있다.
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公开(公告)号：KR1020130083311A
公开(公告)日：2013-07-22
申请号：KR1020120004022
申请日：2012-01-12
Applicant: 서울대학교산학협력단
Abstract: PURPOSE: A method for preparing a transformed bovine embryo using piggybac transposition is provided to uniformly express target genes in all embryos and to stably prepare transformed bovine embryos, thereby studying various embryo-related fields. CONSTITUTION: A transformed bovine embryo is prepared using piggybac transposition. The piggybac transposition is formed using vectors. The piggybac transposition is formed using a primer of sequence number 1 or 2. An embryo is prepared through somatic cell nuclear transfer (SCNT) using fibroblasts as a donor cell. The expression of a foreign target gene is regulated by antibiotics or uniform expression of the foreign target genes.
Abstract translation: 目的：提供使用piggybac转座制备转化牛胚胎的方法，以均匀表达所有胚胎中的靶基因并稳定制备转化的牛胚胎，从而研究各种胚胎相关领域。 构成：使用piggybac转座制备转化的牛胚胎。 使用载体形成piggybac转座。 使用序列号1或2的引物形成piggybac转座。通过使用成纤维细胞作为供体细胞的体细胞核转移（SCNT）制备胚胎。 外源靶基因的表达受抗生素调控或外源靶基因的均一表达。
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