公开(公告)号：KR101209219B1

公开(公告)日：2012-12-06

申请号：KR1020090087825

申请日：2009-09-17

Applicant: 서울대학교산학협력단

Inventor： 김민균 ,  탁제일

IPC: C12N15/29 ,  C12N15/63 ,  C12N15/82

Abstract: 본발명은유전자가도입되어스트레스내성이증진된형질전환식물체에관한것으로, 더욱상세하게는본 발명은벼() 유래의 RAF(root abundant factor) 유전자를포함하는재조합벡터로형질전환된스트레스내성이증진된식물체; 상기식물체의종자;유전자를포함하는재조합벡터로식물세포를형질전환하는단계를포함하는스트레스내성이증진된형질전환식물체의제조방법;유전자를포함하는재조합벡터로식물세포를형질전환시켜식물체의스트레스내성을증진시키는방법; 상기방법에의해제조된스트레스내성이증진된식물체;유전자를포함하는식물체의스트레스내성증진용조성물; 서열번호 2의아미노산서열로이루어진벼() 유래의 RAF 단백질에있어서, 산성영역인 59개 C-말단아미노산으로이루어지는단백질을포함하는전사활성증진용조성물; 및상기유전자를포함하는재조합벡터로식물세포를형질전환시켜식물체의생산성을향상시키는방법에관한것이다.

公开(公告)号：KR1020110029941A

公开(公告)日：2011-03-23

申请号：KR1020090087825

申请日：2009-09-17

Applicant: 서울대학교산학협력단

Inventor： 김민균 ,  탁제일

IPC: C12N15/29 ,  C12N15/63 ,  C12N15/82

Abstract: PURPOSE: A transgenic plant with enhanced stress resistance and a method for preparing the same are provided to enhance the yield of crops and to detect a novel stress resistant gene. CONSTITUTION: A plant with enhanced stress resistance is prepared by transforming with a recombinant vector containing an Oryza sativa-derived RAF(root abundant factor) gene, OsRAF. The stress is ABA(abscisic acid), drought, bacterial diseases, salicylic acid, or methyl jasmonate. A method for preparing a transgenic plant with enhanced stress resistance comprises: a step of transforming a plant cell with the recombinant vector; and a step of redifferentiating the transgenic plant. A composition for enhancing transcription activity contains a protein having 59 C-terminal amino acids.

Abstract translation: 目的：提供具有增强的抗逆性的转基因植物及其制备方法，以提高作物的产量并检测新的抗胁迫基因。 构成：通过用含有水稻衍生的RAF（根丰度因子）基因OsRAF的重组载体转化来制备具有增强的胁迫性的植物。 应激是ABA（脱落酸），干旱，细菌性疾病，水杨酸或茉莉酸甲酯。 一种制备具有增强的抗逆性的转基因植物的方法包括：用重组载体转化植物细胞的步骤; 以及重新分化转基因植物的步骤。 用于增强转录活性的组合物含有具有59个C-末端氨基酸的蛋白质。