公开(公告)号：KR101355128B1

公开(公告)日：2014-01-27

申请号：KR1020120078649

申请日：2012-07-19

Applicant: 서울대학교산학협력단

Inventor： 유상렬 ,  최윤호 ,  한진규

IPC: C12N15/33 ,  C12N1/21 ,  C12N15/70

Abstract: The present invention provides a screening method of Bacillus phage endolysin genes from an Escherichia coli library including the Bacillus phage endolysin genes using T7 phage. By producing a Bacillus phage endolysin gene library using Escherichia coli as a host, and processing the library with T7 phage, the Bacillus phage endolysin genes can be rapidly and economically separated in a non-toxic condition. efficiency.

Abstract translation: 本发明提供了包括使用T7噬菌体的噬菌体内溶素基因的大肠杆菌文库的噬菌体噬菌体内溶素基因的筛选方法。 通过使用大肠杆菌作为宿主制备芽孢杆菌噬菌体内溶素基因文库，并用T7噬菌体处理文库，可以在无毒条件下快速且经济地分离芽孢杆菌噬菌体内溶素基因。 效率。