公开(公告)号：KR1020100130082A

公开(公告)日：2010-12-10

申请号：KR1020090048746

申请日：2009-06-02

Applicant: 서울대학교산학협력단

Inventor： 허원기 ,  성민경 ,  양은빈 ,  이대관 ,  임규범

IPC: C12N1/15 ,  C12N15/31 ,  G01N33/68 ,  C12R1/865

Abstract: PURPOSE: A yeast strain library system for analyzing protein-protein interaction in yeast cell at genome-wide level is provided to quickly analyze the interaction of whole protein of yeast. CONSTITUTION: A gene recombinant Saccharomyces cerevisiae strain library contains yellow fluorescence protein N-terminal fragment gene which is operably linked to C-terminal on a genome DNA of Saccharomyces cerevisiae. A recombinant vector for substituting epitope tag has a sequence of sequence number 1 or 2.

Abstract translation: 目的：提供用于分析全基因组水平的酵母细胞中蛋白质 - 蛋白质相互作用的酵母菌株文库系统，以快速分析酵母全蛋白的相互作用。 构成：基因重组酿酒酵母菌株文库含有黄色荧光蛋白N末端片段基因，其可操作地连接到酿酒酵母基因组DNA上的C末端。 用于替代表位标签的重组载体具有序列号1或2的序列。

公开(公告)号：KR102062744B1

公开(公告)日：2020-01-06

申请号：KR1020170181546

申请日：2017-12-27

Applicant: 서울대학교산학협력단

Inventor： 허원기 ,  장연지

IPC: C12N9/12 ,  C12N9/14 ,  C12N1/16 ,  C07K14/39

公开(公告)号：KR101139589B1

公开(公告)日：2012-04-27

申请号：KR1020090048746

申请日：2009-06-02

Applicant: 서울대학교산학협력단

Inventor： 허원기 ,  성민경 ,  양은빈 ,  이대관 ,  임규범

IPC: C12N1/15 ,  C12N15/31 ,  G01N33/68 ,  C12R1/865

Abstract: PURPOSE: A yeast strain library system for analyzing protein-protein interaction in yeast cell at genome-wide level is provided to quickly analyze the interaction of whole protein of yeast. CONSTITUTION: A gene recombinant Saccharomyces cerevisiae strain library contains yellow fluorescence protein N-terminal fragment gene which is operably linked to C-terminal on a genome DNA of Saccharomyces cerevisiae. A recombinant vector for substituting epitope tag has a sequence of sequence number 1 or 2.

公开(公告)号：KR101875825B1

公开(公告)日：2018-07-06

申请号：KR1020170022508

申请日：2017-02-20

Applicant: 서울대학교산학협력단

Inventor： 허원기 ,  김연수

IPC: C12N15/10 ,  G01N33/68 ,  C40B40/10 ,  C40B50/06

CPC classification number: C12N15/1037 ,  C12N15/1055 ,  C40B40/10 ,  C40B50/06 ,  G01N33/6845

Abstract: 본발명은출아효모 (budding yeast)인사카로마이세스세레비지에 () 세포내에서발현되는단백질들간의상호작용을유전체수준에서분석할수 있는효모균주라이브러리및 그의용도에관한것으로, 더욱상세하게는사카로마이시스세레비지에()의유전체 DNA 상에서단백질을암호화하는유전자의 3'-말단에황색형광단백질의 C-말단절편유전자가작동가능하도록연결된, 사카로마이시스세레비지에() 균주라이브러리, 이의제조방법및 이의단백질상호작용분석용도에관한것이다.

公开(公告)号：KR101029972B1

公开(公告)日：2011-04-20

申请号：KR1020100058213

申请日：2010-06-18

Applicant: 콜마파마(주) ,  서울대학교산학협력단

Inventor： 허원기 ,  송용범 ,  이태규 ,  신동승 ,  정재연 ,  최은욱 ,  안지선

IPC: C12Q1/70 ,  C12N15/86 ,  C12Q1/66 ,  C12Q1/02

CPC classification number: C12N15/86 ,  C12Y105/01003 ,  C12Y302/01023 ,  C12Y304/22044 ,  C12Y305/02006 ,  G01N33/68

Abstract: PURPOSE: A method for real time analysis of protein interaction in animal cells is provided to directly observe interaction and change between proteins by a microscope. CONSTITUTION: A method for measuring interaction of a protein comprises: a step of providing viral genome DNA by cloning a restriction enzyme recognition base sequence, two positions-specific recombinant enzyme recognition base sequence, and a gene encoding a first fragment of a reporter protein; a step of treating the viral genome DNA with a restriction enzyme to prepare two linear viral genome DNA fragments; a step of providing a transport vector containing DNA encoding a first target protein; a step of transducing the recombinant vector to a host cell to prepare the first recombinant virus; a step of providing a second recombinant virus containing a gene encoding a second fragment; and a step of infecting the first and second recombinant viruses to a target cells and measuring coloration.

Abstract translation: 目的：提供一种实时分析动物细胞蛋白质相互作用的方法，通过显微镜直接观察蛋白质之间的相互作用和变化。 构成：用于测定蛋白质相互作用的方法包括：通过克隆限制酶识别碱基序列，两个位置特异性重组酶识别碱基序列和编码报道蛋白的第一个片段的基因来提供病毒基因组DNA的步骤; 用限制酶处理病毒基因组DNA以制备两条线性病毒基因组DNA片段的步骤; 提供含有编码第一靶蛋白的DNA的转运载体的步骤; 将重组载体转导至宿主细胞以制备第一重组病毒的步骤; 提供含有编码第二片段的基因的第二重组病毒的步骤; 以及将第一和第二重组病毒感染到靶细胞并测量着色的步骤。