公开(公告)号：KR101175460B1

公开(公告)日：2012-08-20

申请号：KR1020100068981

申请日：2010-07-16

Applicant: 성균관대학교산학협력단

Inventor： 이창우 ,  이자넷 ,  백선화 ,  이교원 ,  최규실

IPC: C12N5/074 ,  C12N5/079 ,  C12N15/54 ,  A61P25/00

Abstract: 본발명은사이클린의존성인산화효소(cyclin-dependent kinases, CDKs)의활성또는발현의조절에의해성체줄기세포로부터신경세포의교차분화및 신경세포로부터유도된만능줄기세포(induced pluriotent stem cell)로의역분화를유도하는방법에관한발명으로, 기존의신경세포분화유도에사용된첨가물을배제할수 있고, 분화의효율성도높일수 있다는장점이있어퇴행성뇌질환을포함한다양한신경계질환치료기법에응용될수 있을것이다.

公开(公告)号：KR1020120008239A

公开(公告)日：2012-01-30

申请号：KR1020100068981

申请日：2010-07-16

Applicant: 성균관대학교산학협력단

Inventor： 이창우 ,  이자넷 ,  백선화 ,  이교원 ,  최규실

IPC: C12N5/074 ,  C12N5/079 ,  C12N15/54 ,  A61P25/00

Abstract: PURPOSE: A method for differentiation and dedifferentiation between adult stem cells and neural cells is provided to enhance differentiation efficiency and to be applied in various therapeutic methods for treating nervous diseases. CONSTITUTION: A method for inducing differentiation between adult stem cells and neural cells comprises a step of controlling an inhibitor of expression or activation of CDKs(cyclin-dependent kinase). The adult stem cells are differentiated into neural cells by treating the inhibitor. The neural cells are dedifferentiated from neural cells into adult stem cells by removing the inhibitor. The inhibitor includes a compound, siRNA, shRNA, antisense olibonucleotide, or antibody. The shRNA for CDK1, CDK2, and CDK4 contains a base sequence of sequence numbers 13 or 14, 15 or 16, and 17 or 18, respectively.

Abstract translation: 目的：提供成体干细胞和神经细胞分化和去分化的方法，以提高分化效率，并应用于治疗神经疾病的各种治疗方法。 构成：诱导成体干细胞与神经细胞分化的方法包括控制CDK（细胞周期蛋白依赖性激酶）的表达或活化抑制剂的步骤。 通过治疗抑制剂将成体干细胞分化成神经细胞。 通过去除抑制剂，将神经细胞从神经细胞去分化成成体干细胞。 抑制剂包括化合物，siRNA，shRNA，反义寡核苷酸或抗体。 CDK1，CDK2和CDK4的shRNA含有序列号分别为13或14,15或16和17或18的碱基序列。