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公开(公告)号：KR101229307B1
公开(公告)日：2013-02-04
申请号：KR1020110033831
申请日：2011-04-12
Applicant: 성균관대학교산학협력단
Abstract: 본 발명은 특정 단일가닥 핵산이 전극과 결합되어 있는 복합체에 관한 것으로, 본 발명에 의해 제조된 복합체는 LPS를 실시간으로 감지할 수 있으며, 전기화학적 방법으로 쉽고 간단하면서 빠르고 정확하게 추적물질을 감지할 수 있다.
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公开(公告)号：KR1020130081507A
公开(公告)日：2013-07-17
申请号：KR1020120002523
申请日：2012-01-09
Applicant: 성균관대학교산학협력단
CPC classification number: G01N27/3276 , G01N33/5438 , G01N2400/50
Abstract: PURPOSE: A biosensor electrode for detecting lipopolysaccharides (LPS), a forming method thereof, and a reproducing method thereof are provided to implement a biosensor which have high selectivity, high sensitivity, and reproducibility. CONSTITUTION: A forming method of a biosensor electrode for detecting lipopolysaccharides (LPS) is as follows. A metal electrode is provided. A metal electrode / mercaptopropionic acid (MPA) is formed by depositing a 3-MPA layer on the metal electrode, A metal electrode / MPA / NHS is formed by ester-combining the MPA of the metal electrode / MPA and N-hydroxy-succinimide. A metal electrode / MPA / NTA is formed by amide-combining the MPA of the metal electrode / MPA / NHS and NTA-C4-NH2. A metal electrode / MPA / NTA / Cu is formed by covalent-combining copper ions and NTA of the metal electrode / MPA / NTA. [Reference numerals] (AA) Deposition
Abstract translation: 目的：提供一种用于检测脂多糖（LPS）的生物传感器电极及其形成方法及其再现方法，以实现具有高选择性，高灵敏度和再现性的生物传感器。 构成：用于检测脂多糖（LPS）的生物传感器电极的形成方法如下。 提供金属电极。 通过在金属电极上沉积3-MPA层来形成金属电极/巯基丙酸（MPA）。通过将金属电极/ MPA的MPA和N-羟基琥珀酰亚胺酯化合而形成金属电极/ MPA / NHS 。 通过酰胺结合金属电极/ MPA / NHS的MPA和NTA-C4-NH2形成金属电极/ MPA / NTA。 金属电极/ MPA / NTA / Cu通过铜离子和金属电极/ MPA / NTA的NTA共价结合而形成。 （附图标记）（AA）沉积
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公开(公告)号：KR1020130081510A
公开(公告)日：2013-07-17
申请号：KR1020120002526
申请日：2012-01-09
Applicant: 성균관대학교산학협력단
CPC classification number: G01N27/3275 , G01N33/5438 , G01N33/566 , G01N2333/485 , G01N2333/71
Abstract: PURPOSE: A biosensor electrode for detecting HER2, a forming method thereof, and a reproducing method thereof are provided to detect HER2 with high sensitivity by using a new method. CONSTITUTION: A forming method of a biosensor electrode for detecting HER2 is as follows. A metal electrode is provided. A metal electrode / mercaptopropionic acid (MPA) is formed by depositing a 3-MPA layer on the metal electrode. A metal electrode / MPA / NHS is formed by ester-combining the MPA of the metal electrode / MPA and N-hydroxy-succinimide. A metal electrode / MPA / aptamer is formed by amide-combining HER2 specific aptamer with an amino group terminal and the MPA of the metal electrode / MPA / NHS / aptamer. [Reference numerals] (AA) Gold electrode; (BB) Mechanical grinding; (CC) Electromechanical grinding; (DD) CV measurement; (EE) MPA self-assembly; (FF) Fixing; (GG) EIS measurement
Abstract translation: 目的：提供一种用于检测HER2的生物传感器电极及其形成方法及其再现方法，以通过使用新的方法以高灵敏度检测HER2。 构成：用于检测HER2的生物传感器电极的形成方法如下。 提供金属电极。 通过在金属电极上沉积3-MPA层来形成金属电极/巯基丙酸（MPA）。 通过将金属电极/ MPA的MPA和N-羟基 - 琥珀酰亚胺酯化而形成金属电极/ MPA / NHS。 通过酰胺结合HER2特异性适体与氨基末端和金属电极/ MPA / NHS /适体的MPA形成金属电极/ MPA /适体。 （标号）（AA）金电极; （BB）机械研磨; （CC）机电研磨; （DD）CV测量; （EE）MPA自组装; （FF）固定; （GG）EIS测量
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公开(公告)号：KR1020120116224A
公开(公告)日：2012-10-22
申请号：KR1020110033831
申请日：2011-04-12
Applicant: 성균관대학교산학협력단
CPC classification number: G01N33/50 , C12Q1/6825 , G01N27/302 , G01N2400/50
Abstract: PURPOSE: A biosensor for sensing lipopolysaccharide and a manufacturing method thereof are provided to sense LPS in real time basis and to easily and rapidly sense detecting materials. CONSTITUTION: A biosensor for sensing lipopolysaccharide is represented by chemical formula 1. In the composite, specific single stranded nucleic acid is combined with electrode. In the chemical formula 1, E indicates the electrode. R1 indicates the alkylene with 1-12 carbons. The ssDNA indicates the single stranded nucleic acid represented by the sequence number 1(SEQ ID NO:1). The electrode is gold, platinum or graphite. A manufacturing method of the composite of the chemical formula 1 comprises the following steps: reacting a compound which is represented by chemical formula 2 with the electrode; reacting the manufactured reactant with carboamide compound which is represented by chemical formula 3; and reacting the manufactured reactant with the single stranded nucleic acid represented by the sequence number 1 in which amine group is combined with 5' part. The manufacturing method additionally performs a step of polishing the electrode before the first step.
Abstract translation: 目的：提供一种用于感测脂多糖的生物传感器及其制造方法，用于实时感测LPS并容易且快速地感测检测材料。 构成：用于感测脂多糖的生物传感器由化学式1表示。在复合物中，将特定的单链核酸与电极组合。 在化学式1中，E表示电极。 R1表示具有1-12个碳原子的亚烷基。 ssDNA表示由序列号1（SEQ ID NO：1）表示的单链核酸。 电极是金，铂或石墨。 化学式1的复合物的制造方法包括以下步骤：将由化学式2表示的化合物与电极反应; 使所制备的反应物与由化学式3表示的氨基甲酰化合物反应; 并使所制备的反应物与其中胺基与5'部分组合的序列号1表示的单链核酸反应。 该制造方法还执行在第一步骤之前抛光电极的步骤。
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