oxalicum ) KL1 (기탁번호 KACC93151P) 및 이를 이용한 목질섬유소분해효소의 제조방법에 관한 것으로, 보다 상세하게는 썩은 나무 부스러기를 포함하는 토양으로부터 분리한 페니실리움 옥살리쿰 KL1 및 이를 배양하는 것을 특징으로 하는 목질섬유소분해효소의 경제적인 제조방법에 관한 것이다. 본 발명에서는 목질섬유소를 당화하여 포도당과 자일로스 등의 단당류를 제조하기 위해 기존 공지의 강력한 효소 생산 미생물보다 여러가지 목질섬유소분해 효소활성이 더 증진된 목질섬유소분해효소를 생산하는 곰팡이 균주인 페니실리움 옥살리쿰 KL1이 제공되며, 이를 사용하여 저가의 천연 섬유질 원료로부터 경제적인 목질섬유소분해효소를 생산하는 방법이 제공되며, 목질섬유소로부터 포도당과 자일로스 등의 단당류를 생산하는 방법이 제공된다. 이 발명에 의해 목질섬유소 기질로부터 산업적으로 유용한 포도당과 자일로스 등의 단당류를 생산하는 데 필요한 효소비용을 크게 절감할 수 있다.
Abstract:
PURPOSE: A new fungal strain, Lecythophora sp. YP363, and a method for producing thermostable cellulase using the same are provided to produce monosaccharides such as glucose and xylose from a cellulose substrate and to reduce enzyme cost. CONSTITUTION: A fungal strain, Lecythophora sp. YP363 (deposit number: KACC93143P), has an excellent enzyme activity and thermal resistance and produces cellulase. A method for preparing cellulase comprises the steps of culturing the strain and isolating cellulase from the culture. The optimal temperature for cellulase is 55 °C. The carbon source in a culture medium is selected among Miscanthus sinensis var. purpurascens, Phragmites communis TRIN., palm fruit peels, barley straw, and rice straw. Cellulase is used for preparing glucose and xylose from a ligneous ingredient.
Abstract:
The present invention relates to a fungal strain Penicillium oxalicum KL1 (accession no. KACC93151P) and a production method of lignocellulose-degrading enzymes using the same, and more particularly, to a fungal strain Penicillium oxalicum KL1 isolated from soil containing decayed tree fragments and an economical production method of lignocellulose-degrading enzymes culturing the strain. The present invention provides the fungal strain Penicillium oxalicum KL1, which produces several lignocellulose-degrading enzymes having more enhanced enzymatic activity than the known potent enzyme-producing microorganisms, in order to saccharify wooden cellulose to prepare monosaccharides, such as glucose and xylose, and provides an economical production method of lignocellulose-degrading enzymes from low-cost natural fibrous materials. Therefore, the costs of enzymes necessary for producing industrially useful monosaccharides, such as glucose and xylose, from lignocelluloses can be reduced.
Abstract:
PURPOSE: A fermentation method for preparing fermented fucoidan as a cosmetic material is provided to enhance functionality such as collagen synthesis, whitening, and MMP(matrix metalloproteinase) suppression. CONSTITUTION: A method for preparing fermented fucoidan with enhanced anti-aging functionality, collagen synthesis, whitening, and MMP suppression comprises a step of fermenting with microorganisms such as Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae. The fermented fucoidan is used as a raw material of a cosmetic product, a food product, and a pharmaceutical product. The fucoidan is polymer fucoidan which is isolated from kelp with a purity of 80%.