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公开(公告)号：KR1020070109347A
公开(公告)日：2007-11-15
申请号：KR1020060042197
申请日：2006-05-11
Applicant: 숙명여자대학교산학협력단
Abstract: A hybridoma cell is provided to secret a monoclonal antibody capable of specifically recognizing human CTRP1(C1q-TNF related protein-1) and immunoprecipitating it. A monoclonal antibody is provided to detect the CTRP1 protein economically and conveniently and be widely used for diagnosing, preventing and treating obesity or diabetes. A hybridoma cell E21H7 produces mouse monoclonal antibodies binding to human CTRP1 and is deposited as a deposition no. KCTC 10944BP. A method for producing the hybridoma cell E21H7 comprises the steps of: (a) immunizing mouse using a fusion protein of human CTRP1; (b) fusing a spleen cell of the immunized mouse into myeloma cell and then culturing it; and (c) selecting hybridoma cells producing monoclonal antibodies specific to the human CTRP1 from the fused hybridoma cells. An IgG2a type monoclonal antibody specific to human CTRP1 is prepared by the hybridoma cell E21H7.
Abstract translation: 提供了一种杂交瘤细胞来保护能够特异性识别人类CTRP1（C1q-TNF相关蛋白-1）并免疫沉淀的单克隆抗体。 提供单克隆抗体经济地和方便地检测CTRP1蛋白，被广泛用于诊断，预防和治疗肥胖或糖尿病。 杂交瘤细胞E21H7产生与人CTRP1结合的小鼠单克隆抗体，并作为沉积物沉积。 KCTC 10944BP。 制备杂交瘤细胞E21H7的方法包括以下步骤：（a）使用人CTRP1的融合蛋白免疫小鼠; （b）将免疫小鼠的脾细胞融合入骨髓瘤细胞，然后培养; 和（c）从融合的杂交瘤细胞中选择产生对人CTRP1特异性的单克隆抗体的杂交瘤细胞。 通过杂交瘤细胞E21H7制备对人CTRP1特异性的IgG2a型单克隆抗体。
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3.发明授权인간 씨티알피1 유전자의 에스아이알엔에이, 및 이를포함하는 고혈압 및 심혈관계 질환 치료용 약학조성물 有权人CTRP1基因的siRNA，以及包含用于治疗高血压和心血管疾病的siRNA的药物组合物
公开(公告)号：KR100976170B1
公开(公告)日：2010-08-16
申请号：KR1020070128640
申请日：2007-12-12
Applicant: 숙명여자대학교산학협력단
Abstract: 본 발명은 인간 CTRP1 단백질의 생성을 억제하는 인간 CTRP1 유전자의 siRNA 및 이를 포함하는 고혈압 또는 심혈관질환 치료용 약학조성물을 제공한다. 본 발명은 또한 인간 CTRP1 유전자 또는 인간 CTRP1 단백질을 포함하는 고혈압 또는 심혈관질환 치료제 스크리닝 조성물 및 이를 이용한 스크리닝 방법을 제공한다. 추가로, 본 발명은 인간 CTRP1 단백질에 대한 항체를 포함하는 고혈압 또는 심혈관질환 진단 조성물 및 이를 포함하는 고혈압 또는 심혈관질환 진단용 키트를 제공한다.
CTRP1, 알도스테론, 고혈압, 심혈관질환-
4.发明公开인간 씨티알피1 유전자의 에스아이알엔에이, 및 이를포함하는 고혈압 및 심혈관계 질환 치료용 약학조성물 有权人类CTRP1基因的SIRNA和包含SIRNA用于治疗高血压和心脏血管疾病的药物组合物
公开(公告)号：KR1020090061727A
公开(公告)日：2009-06-17
申请号：KR1020070128640
申请日：2007-12-12
Applicant: 숙명여자대학교산학협력단
Abstract: A pharmaceutical compound for treating hypertension or cardiovascular diseases, which contains siRNA(single interfering RNA) of human CTRP1(complement C1q TNF related protein 1) is provided to inhibit generation of aldosterone by suppressing the human CTRP1 generation. A siRNA of human CTRP1 gene which suppresses the generation of human CTRP1 comprises polynucleotide pair of the sequence numbers 3(SEQ ID NO:3) and 4, or 5 and 6. A pharmaceutical composition for treating hypertension or cardiovascular diseases comprises the siRNA of human CTRP1 gene as an inhibition material of human CTRP1 generation. The pharmaceutical composition comprises a human CTRP1 antibody as an inhibitor of human CTRP1 function. The human CTRP1 antibody is produced by a hybridoma cell line E21H7. A composition for screening the drug for hypertension or cardiovascular diseases comprises the human CTRP1 gene or protein.
Abstract translation: 提供含有人CTRP1（补体C1q TNF相关蛋白1）的siRNA（单干扰RNA）的高血压或心血管疾病的药物化合物，通过抑制人类CTRP1的产生来抑制醛固酮的产生。 抑制人CTRP1产生的人CTRP1基因的siRNA包含序列号3（SEQ ID NO：3）和4或5和6的多核苷酸对。用于治疗高血压或心血管疾病的药物组合物包含人的siRNA CTRP1基因作为人类CTRP1生成的抑制物质。 药物组合物包含人CTRP1抗体作为人CTRP1功能的抑制剂。 人类CTRP1抗体由杂交瘤细胞系E21H7产生。 用于筛选高血压或心血管疾病药物的组合物包括人类CTRP1基因或蛋白质。
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