公开(公告)号：KR101903770B1

公开(公告)日：2018-10-08

申请号：KR1020170071478

申请日：2017-06-08

Applicant: 원광대학교산학협력단

Inventor： 이승재 ,  조영삼 ,  정훈진 ,  강내운 ,  이세환 ,  김소연

IPC: C12M3/00 ,  C12M1/12 ,  C12M1/00 ,  C12M1/26

CPC classification number: C12M21/08 ,  C12M25/00 ,  C12M29/00 ,  C12M33/00

Abstract: 세포배양시스템이개시된다. 본발명의실시예에따른세포배양시스템은세포지지체를표면에수용한제1 관절부및 제2 관절부가서로대향하여위치하는관절부와, 관절부를수용하며세포지지체에서세포를배양하는배양챔버와, 제1 관절부에연장되어결합하는제1 뼈대와, 제2 관절부에연장되어결합하는제2 뼈대와, 제1 뼈대의일단이결합하는제1 지지부와, 제2 뼈대의일단이결합하는제2 지지부와, 제1 지지부및 제2 지지부가슬라이드결합하며수직이동하는메인축을각각포함하는복수의인체관절모사(simulation)부; 및복수의인체관절모사부의제2 뼈대마다연결되어, 제2 뼈대의각도를조절하는굽힘각도조절부를포함한다.

公开(公告)号：KR1020130126214A

公开(公告)日：2013-11-20

申请号：KR1020120050126

申请日：2012-05-11

Applicant: 원광대학교산학협력단 ,  충북대학교 산학협력단

Inventor： 이승재 ,  김소연 ,  이인환

IPC: C12M3/02 ,  C12N5/071

CPC classification number: C12M23/48 ,  C12M29/00 ,  C12N5/0655

Abstract: Cell culture system and method of a biomimetic bioreactor are disclosed. The cell culture system which cultures joint cells comprises: a joint part which comprises a first joint part in which a first scaffold conjugated on the surface and a second joint part in which a second scaffold is conjugated on the surface facing the first joint part; a culture chamber which receives the joint part and is able to culture cells on a first artificial support and a second artificial support; and a storage unit which is connected with the culture chamber by a connection tube supplies a culture medium for culturing the cells to the culture chamber. The cell culture system is able to effectively culture chondrocytes by realizing a culture medium circulation method, a joint movement structure, a scaffold insertion structure, and a chondrocyte culture to make an optimal culture environment in an in vitro culture chamber in which chondrocytes are cultured.

Abstract translation: 公开了仿生生物反应器的细胞培养系统和方法。 培养关节细胞的细胞培养系统包括：接头部分，其包括第一接合部分，其中在表面上共轭的第一支架和第二接合部分，其中第二支架在面向第一接合部分的表面上共轭; 培养室，其容纳所述接合部，能够在第一人造支撑体和第二人造支撑体上培养细胞; 并且通过连接管与培养室连接的存储单元将用于培养细胞的培养基提供给培养室。 细胞培养系统能够通过实现培养基循环方法，关节运动结构，支架插入结构和软骨细胞培养来有效培养软骨细胞，从而在培养软骨细胞的体外培养室中形成最佳的培养环境。

公开(公告)号：KR1020130126216A

公开(公告)日：2013-11-20

申请号：KR1020120050128

申请日：2012-05-11

Applicant: 원광대학교산학협력단 ,  충북대학교 산학협력단

Inventor： 이승재 ,  김소연 ,  이인환

IPC: C12M3/02 ,  C12N5/071

CPC classification number: C12M23/48 ,  C12M29/00 ,  C12N5/0655

Abstract: An operation system for cell culture of a biomimetic bioreactor is disclosed. The operation system for culturing joint cells comprises: a joint part with a first joint part in which a first scaffold is conjugated on the surface thereof and a second joint part facing the first joint part, in which a second scaffold is conjugated on the surface thereof; a culture chamber which receives the joint part and is used for culturing cells on the first scaffold and the second scaffold; a first rod which is extended and coupled to the first joint part; a second rod which is extended and coupled to the second joint part facing the first joint part; and a bending angle adjusting unit which adjusts the angle of the first and second rods. The operation system for cell culture is able to effectively culture chondrocytes by forming a joint structure for a culture medium circulation method, a joint movement structure, a scaffold insertion structure, and a chondrocyte culture to make an optimal culture condition in an in vitro culture chamber in which chondrocytes are cultured.

Abstract translation: 公开了一种仿生生物反应器的细胞培养操作系统。 用于培养关节细胞的操作系统包括：具有第一接合部分的接合部分，第一接合部分在其表面上共轭第一支架和面向第一接合部分的第二接合部分，其中第二个支架在其表面上共轭 ; 培养室，其接收所述关节部分，并用于在所述第一支架和所述第二支架上培养细胞; 第一杆，其延伸并联接到所述第一接合部; 第二杆，其延伸并联接到面向所述第一接合部的所述第二接合部; 以及调整第一和第二杆的角度的弯曲角度调节单元。 用于细胞培养的操作系统能够通过形成用于培养基循环方法，关节运动结构，支架插入结构和软骨细胞培养物的接合结构来有效培养软骨细胞，以在体外培养室中形成最佳培养条件 其中培养软骨细胞。