公开(公告)号：KR101256581B1

公开(公告)日：2013-04-19

申请号：KR1020110107543

申请日：2011-10-20

Applicant: 서울대학교산학협력단 ,  이티바이오텍주식회사

Inventor： 노상호 ,  정연길 ,  김세웅

IPC: A61D19/04 ,  A61P29/00

CPC classification number: A61D19/04 ,  A01K67/02 ,  A61M5/14

Abstract: PURPOSE: A method for transferring a fertilized egg of cows is provided to improve pregnancy rate in cows by suppressing an inflammatory reaction with dexamethasone and rhLIF, thereby increasing productivity of Korean beef cattle. CONSTITUTION: A method for transferring a fertilized egg of cows comprises the following steps: (a) externally aging a cow egg, externally fertilizing the egg with sperm, and externally culturing the fertilized egg; (b) putting the fertilized egg in a transferring solution containing dexamethasone and a recombinant human leukemia inhibitory factor(rhLIF), and loading the fertilized egg and the solution in straw; and (c) directly injecting the straw loaded with the fertilized egg and the solution, and transferring the fertilized egg. [Reference numerals] (1) Verifying based on the occurrence of re-estrus between 23 days and 42 days; (2) Analyzing pregnancy when 60 days after the pregnancy; (AA) Solution for transferring a fertilized egg; (BB) Basic solution(TCM-199 + 10% FBS); (CC) 100nM/ml Dexamethasone and 100nM/ml rhLIF; (DD) Washing twice to three times; (EE) Washing using the same solution; (FF) Loading a straw; (GG) Loading a fertilized egg and the solution for transferring a fertilized egg in the straw; (HH) Transferring the fertilized egg; (II) Analyzing pregnancy;

Abstract translation: 目的：提供转移奶牛受精卵的方法，通过抑制地塞米松和rhLIF的炎症反应，提高牛的妊娠率，从而提高韩国肉牛的生产力。 构成：一种转移奶牛受精卵的方法，包括以下步骤：（a）外部老化牛卵，外部用精子对卵进行施肥，外部培养受精卵; （b）将受精卵置于含有地塞米松和重组人白血病抑制因子（rhLIF）的转移溶液中，并将受精卵和溶液装入秸秆中; 和（c）直接注入装有受精卵和溶液的秸秆，并转移受精卵。 （附图标记）（1）根据23天〜42天的发情情况进行验证; （2）怀孕60天后分析怀孕; （AA）转移受精卵的方法; （BB）碱性溶液（TCM-199 + 10％FBS）; （CC）100nM / ml地塞米松和100nM / ml rhLIF; （DD）洗涤两次至三次; （EE）使用相同的溶液洗涤; （FF）装载吸管; （GG）装载受精卵和将受精卵转移到秸秆中的溶液; （HH）转移受精卵; （二）分析怀孕;

公开(公告)号：KR101934969B1

公开(公告)日：2019-01-03

申请号：KR1020160172730

申请日：2016-12-16

Applicant: 이티바이오텍주식회사 ,  서울대학교산학협력단

Inventor： 김대환 ,  노상호 ,  정연길

IPC: A01N1/02 ,  C12N5/076