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公开(公告)号：WO2009104907A2
公开(公告)日：2009-08-27
申请号：PCT/KR2009/000783
申请日：2009-02-18
IPC: C12N5/08
CPC classification number: C12N5/0695 , C12N2501/11 , C12N2501/115 , C12N2501/235
Abstract: 본 발명은 유방암 조직으로부터 유래되고 유방암 줄기세포 표지 인자를 발현하는 다능성 암 줄기 세포주 및 (1) 기 채취된 유방암 조직으로부터 유방암 세포를 분리하는 단계; (2) 상기 분리된 유방암 세포를 부유 배양용 배지에서 부유 상태로 일차 배양하는 단계; (3) 상기 일차 배양물로부터 부유 상태의 세포들을 회수하는 단계; 및 (4) 회수된 세포들을 부유 배양용 배지에서 부유 상태로 일정 회수 이상 계대 배양하여 다능성 암 줄기 세포주를 제조하는 단계를 포함한 다능성 암 줄기 세포주의 제조방법에 관한 것이다.
Abstract translation:
本发明包括分离的多能干细胞的乳腺癌细胞的步骤，和（1）从乳腺癌组织衍生的基团所收集，并表示从乳腺癌组织乳腺癌干细胞标记物; （2）在悬浮培养基中以漂浮状态初次培养分离的乳腺癌细胞; （3）从原代培养物中回收悬浮细胞; （4）将回收的细胞在悬浮培养基中悬浮培养预定次数或更多次以产生多能癌症干细胞系。
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公开(公告)号：KR1020100011766A
公开(公告)日：2010-02-03
申请号：KR1020080073115
申请日：2008-07-25
Applicant: 재단법인서울대학교산학협력재단
Abstract: PURPOSE: A target therapeutic agent of cancer stem cells using berberine is provided to suppress the expression of drug resistance target factor and induces apoptosis of cancer stem cells. CONSTITUTION: A target therapeutic agent of cancer stem cells contains berberine of chemical formula 1, its derivative or their pharmaceutically acceptable salt as an active ingredient. In chemical formula 1, X is halogen atom or sulfate. The cancer stem cell is breast cancer stem cells. The breast cancer cells express target factor of breast cancer stem cell, target factor of breast cancer resistance drug, target factor of breast cancer diagnostic receptor, target factor of epithelial cells, or target factor of neural cells. The breast cancer stem cell is deposit number KCLRF-BP-00137, KCLRF-BP-00138 or KCLRF-BP-00174. A food composition for preventing or treating cancer stem cells contains the berberine of chemical formula 1, its derivative or their salt as an active ingredient.
Abstract translation: 目的：提供使用小檗碱的癌干细胞靶向治疗剂，抑制药物抗性靶因子的表达，诱导癌细胞凋亡。 构成：癌干细胞的靶治疗剂含有化学式1的小檗碱，其衍生物或其药学上可接受的盐作为活性成分。 在化学式1中，X是卤素原子或硫酸根。 癌症干细胞是乳腺癌干细胞。 乳腺癌细胞表达乳腺癌干细胞的靶因子，乳腺癌抗药性的靶因子，乳腺癌诊断受体的靶因子，上皮细胞的靶因子或神经细胞的靶因子。 乳腺癌干细胞是保藏号KCLRF-BP-00137，KCLRF-BP-00138或KCLRF-BP-00174。 用于预防或治疗癌症干细胞的食物组合物含有化学式1的小檗碱，其衍生物或其盐作为活性成分。
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公开(公告)号：KR1020090089120A
公开(公告)日：2009-08-21
申请号：KR1020080014498
申请日：2008-02-18
Applicant: 재단법인서울대학교산학협력재단
CPC classification number: C12N5/0695 , C12N2501/11 , C12N2501/115 , C12N2501/235
Abstract: A method for manufacturing multipotential cancer stem cell line is provided to overcome anticancer drug resistance and maximize the efficiency of treating cancer. A multipotential cancer cell line is derived from breast cancer tissue and expresses a marker of breast cancer stem cell. The breast cancer tissue is sarcoma. The marker is CD24low/-CD44high. A method for producing the mutipotential cancer stem cell line comprises: a step of isolating a breast cancer cell from a breast cancer tissue; a step of first-culturing isolated breast cancer cell in floating medium; a step of collecting floated cells; and a step of sub-culturing the cells several times to produce mutipotential cancer stem cell line.
Abstract translation: 提供了一种制造多潜能癌干细胞系的方法，以克服抗癌药物的耐药性并最大限度地提高治疗癌症的效率。 多能癌细胞系来自乳腺癌组织，表达乳腺癌干细胞的标志物。 乳腺癌组织是肉瘤。 标记是CD24low / -CD44high。 产生多能性癌干细胞系的方法包括：从乳腺癌组织中分离乳腺癌细胞的步骤; 在浮动培养基中首先培养分离的乳腺癌细胞的步骤; 收集浮游细胞的一个步骤; 以及将细胞分次数次培养以产生多能性癌干细胞系的步骤。
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