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公开(公告)号：KR1020080084101A
公开(公告)日：2008-09-19
申请号：KR1020070025280
申请日：2007-03-15
Applicant: 재단법인서울대학교산학협력재단
Abstract: A method for culturing an embryo in early mammal pregnancy using kinetin is provided to increase the rate of expanded and hatched blastocysts, growth rate and survivability of the blastocysts. A method for developing an embryo in early mammal pregnancy using NCSU(North Carolina State University-23 medium)-PVA(polyvinylalcohol) comprises a step of culturing the embryo in a culture solution of NCSU(North Carolina State University-23 medium)-PVA(polyvinylalcohol) where 100-800muM/L of kinetin is added at a temperature of 37-39 deg.C for 5-7 days.
Abstract translation: 提供了使用激动素在早期哺乳动物妊娠中培养胚胎的方法，以增加扩大和孵出的囊胚的速率，生长速率和胚泡的生存能力。 使用NCSU（北卡罗来纳州州立大学-23培养基）-PVA（聚乙烯醇）在早期哺乳动物怀孕中发育胚胎的方法包括在NCSU（北卡罗来纳州立大学-23培养基）-PVA培养溶液中培养胚胎的步骤 （聚乙烯醇），其中在37-39℃的温度下加入100-800muM / L的激动素5-7天。
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公开(公告)号：KR100793680B1
公开(公告)日：2008-01-10
申请号：KR1020060089708
申请日：2006-09-15
Applicant: 재단법인서울대학교산학협력재단
Abstract: A method for culturing an embryo is provided to improve the development of the embryo before the implantation of a mammal by using an oil-free culture medium, thereby being usefully used for developing embryos of various mammals. To culture an embryo using a container which consists of a lower portion closed microchannel, the container is one selected from the group consisting of a container, the lower portion of the microchannel having a shape of gradually being narrowed and a closing cap being formed on the upper portion of the microchannel, a container, the lower portion of the microchannel having a U-shape or a corn shape, and a container, the diameter of the microchannel being 0.1-0.2 cm and the length of the lower portion which is gradually narrowed is 1-3 cm. In the method, a culture medium is oil-free, the number of the embryo in the container is 1-8/mul, and the embryo is an embryo of mammal.
Abstract translation: 提供培养胚胎的方法，以通过使用无油培养基在植入哺乳动物之前改善胚胎的发育，从而有用地用于开发各种哺乳动物的胚胎。 为了使用由下部封闭的微通道构成的容器来培养胚胎，容器是从容器中选出的一个，微通道的下部具有逐渐变窄的形状，并且封闭帽形成在 微通道的上部，容器，微通道的下部具有U形或玉米形状，以及容器，微通道的直径为0.1-0.2cm，下部的长度逐渐变窄 是1-3厘米。 在该方法中，培养基无油，容器中的胚胎数为1-8个/个，胚胎为哺乳动物的胚胎。
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公开(公告)号：KR100869933B1
公开(公告)日：2008-11-24
申请号：KR1020070025280
申请日：2007-03-15
Applicant: 재단법인서울대학교산학협력재단
Abstract: 본 발명은 키네틴을 이용한 포유동물의 임신초기 배아의 배양에 관한 것으로, 완전한정배양액 (NCSU-PVA) 내에서 포유동물의 단위발생란 및 핵이식란중 어느 하나를 발달시키는데 있어, 상기 완전한정배양액에는 키네틴이 특정 함량 포함되는 것을 특징으로 한다.
본 발명에 의하면, 식물세포의 세포분열을 촉진시키는 시토키닌으로 알려진 식물 호르몬인 키네틴이 포유동물의 임신초기 배아의 배양시 첨가되면 확장 및 부화된 배반포 비율, 배반포의 성장속도 및 생존성을 증가시키는 등 배양에 효과적인 것을 확인할 수 있다.
완전한정배양액, 포유동물, 배아, 배반포, 키네틴
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