公开(公告)号：KR1020080051316A

公开(公告)日：2008-06-11

申请号：KR1020060122170

申请日：2006-12-05

Applicant: 재단법인서울대학교산학협력재단

Inventor： 황인규 ,  문제선

IPC: A01N63/02 ,  A01N63/00

CPC classification number: A01N63/02 ,  A01N43/90 ,  C12N1/20 ,  C12R1/01

Abstract: Toxoflavin isolated from Burkholderia glumae is provided to remove selectively weed species without environment pollution and toxicity to human and domestic animals, so that the substance is useful as an environment-friendly biopesticide. The herbicide contains the cultured medium of Burkholderia glumae or 10-1000 muM of toxoflavin or its derivatives isolated from the cultured medium of Burkholderia glumae, and agriculturally acceptable carriers. A method for separating the toxoflavin from Burkholderia glumae comprises the steps of: (1) removing cells from the cultured medium of Burkholderia glumae through centrifugation and obtaining the supernatant; and (2) extracting the supernatant with chloroform, and optionally evaporating chloroform from the toxoflavin extract and dissolving it in methanol.

Abstract translation: 提供从伯克霍尔德氏菌中分离出的黄曲霉毒素可以选择性去除杂种，而不会对人类和家畜造成环境污染和毒性，因此该物质可用作环境友好的生物农药。 该除草剂含有从伯克霍尔德氏菌的培养培养基中分离的伯克霍尔德氏菌属培养基或10-1000μM的毒黄色素或其衍生物，以及农业上可接受的载体。 从白僵菌中分离毒黄素的方法包括以下步骤：（1）通过离心分离从白念珠菌的培养基中除去细胞并获得上清液; 和（2）用氯仿提取上清液，并任选地从毒素黄酮提取物中蒸发氯仿并将其溶解在甲醇中。

公开(公告)号：KR1020080051240A

公开(公告)日：2008-06-11

申请号：KR1020060121964

申请日：2006-12-05

Applicant: 재단법인서울대학교산학협력재단 ,  한국생명공학연구원

Inventor： 황인규 ,  문제선 ,  김성욱

IPC: C12N15/11 ,  C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/18 ,  C12N15/11

Abstract: A screening system of inhibitors and activators of type III secretion machinery which directly transfers pathogenic proteins of bacteria into a host cell in gram-negative bacteria is provided to identify substances capable of activating or inhibiting the secretion of type III protein secretion system through the expression of a reporter gene, so that pathogenic bacterial infection is controlled. A screening system of inhibitors and activators of type III secretion machinery in gram-negative bacteria comprises the steps of: preparing a plasmid pLAFR6-trc:HpaG:GUS containing hpaG(hypersensitivity response-related gene) and GUS(beta-glucuronidase) genes; transforming a host cell, Xanthomonas axonopodis pv. glycines with the plasmid; culturing the transformed cell in XVM2 induction medium to induce secretion of hpaG; and verifying the secretion of hpaG by using GUS dyeing.

Abstract translation: 提供将细菌病原性蛋白质直接转移到革兰氏阴性细菌宿主细胞中的抑制剂和激活剂的筛选系统，以鉴定能够通过表达形式表达III型蛋白质分泌系统的活化或抑制分泌的物质 记者基因，使病原菌感染得到控制。 革兰氏阴性细菌中III型分泌机制的抑制剂和激活剂的筛选系统包括以下步骤：制备含有hpaG（超敏反应相关基因）和GUS（β-葡糖醛酸糖苷酶）基因的质粒pLAFR6-trc：HpaG：GUS; 转化宿主细胞，Xanthomonas axonopodis pv。 甘氨酸与质粒; 在XVM2诱导培养基中培养转化细胞以诱导hpaG分泌; 并通过使用GUS染色验证hpaG的分泌。

公开(公告)号：KR100781061B1

公开(公告)日：2007-11-30

申请号：KR1020060121972

申请日：2006-12-05

Applicant: 재단법인서울대학교산학협력재단

Inventor： 황인규 ,  문제선

IPC: C12N9/00 ,  C12N9/14

Abstract: A toxoflavin-degrading enzyme tf1A is provided to select a transgenic plant simply and efficiently. And a method for selecting a transgenic plant and a method for preparing a transgenic plant using the tf1A are provided. A recombinant Arabidopsis thaliana expression vector includes a tf1A gene consisting of a sequence of SEQ ID : NO. 1 and has a cleavage map depicted in Fig. 2. A method for selecting a transgenic plant comprises the steps of: (a) transforming a plant using the recombinant vector including the tf1A gene; and (b) proliferating the transgenic plant in a culture medium containing toxoflavin. A method for preparing a transgenic plant comprises the steps of: (a) transforming a plant cell using the recombinant vector including the tf1A gene; (b) proliferating the transformed plant cell in the culture medium containing toxoflavin; and (c) redifferentiating the transformed plant cell into a transgenic plant.

Abstract translation: 提供毒黄色素降解酶tf1A来简单有效地选择转基因植物。 提供了选择转基因植物的方法和使用tf1A制备转基因植物的方法。 重组拟南芥表达载体包含由SEQ ID NO：1的序列组成的tf1A基因。 并具有图1所示的切割图。 2.选择转基因植物的方法包括以下步骤：（a）使用包含tf1A基因的重组载体转化植物; 和（b）在含有毒素黄素的培养基中增殖转基因植物。 制备转基因植物的方法包括以下步骤：（a）使用包含tf1A基因的重组载体转化植物细胞; （b）在含有毒黄素的培养基中增殖转化的植物细胞; 和（c）将转化的植物细胞重新分化为转基因植物。

公开(公告)号：KR100781066B1

公开(公告)日：2007-11-30

申请号：KR1020060088580

申请日：2006-09-13

Applicant: 재단법인서울대학교산학협력재단

Inventor： 황인규 ,  문제선

IPC: C12N15/29 ,  C07K14/415

Abstract: An rsa1 gene involving with the host specificity of Ralstonia solanacearum is provided to understand the occurrence mechanism of bacterial wilt and present a differential controlling method regarding the Ralstonia solanacearum through breeding of a resistant plant, thereby efficiently decreasing the occurrence of the bacterial wilt. An rsa1 gene plays an important role in host specificity of recognizing host in Ralstonia solanacearum race 3 strain which is bacterial wilt and includes a sequence of SEQ ID : NO. 1. An rsa1 protein includes an amino acid sequence of SEQ ID : NO. 2. A DNA encodes the rsa1 gene. A protein encoding the rsa1 gene includes an amino acid sequence of SEQ ID : NO. 2. A plasmid pRS13 includes the rsa1 gene.

Abstract translation: 提供涉及Ralstonia solanacearum的宿主特异性的rsa1基因，以了解细菌枯萎病的发生机制，并通过抗性植物的育种呈现关于Ralstonia solanacearum的差异控制方法，从而有效降低细菌枯萎病的发生。 rsa1基因在识别宿主的宿主特异性中起重要作用，该菌属是细菌枯萎病（Ralstonia solanacearum race 3）菌株，其包含SEQ ID NO： rsa1蛋白包括SEQ ID NO：1的氨基酸序列。 DNA编码rsa1基因。 编码rsa1基因的蛋白质包含SEQ ID NO： 质粒pRS13包含rsa1基因。