公开(公告)号：KR100460190B1

公开(公告)日：2004-12-04

申请号：KR1020000053341

申请日：2000-09-08

Applicant: 재단법인서울대학교산학협력재단

Inventor： 박상철 ,  박정수 ,  박웅양

IPC: C12Q1/00

Abstract: PURPOSE: Provided are method for detecting senescent cells using amphiphysin 1 protein and its gene, a composition for restoring a physiological function of senescent cells and a method for restoring a physiological function of senescent cells. CONSTITUTION: The method for detecting senescent cells in mammal cells is characterized by measuring the decrease of amphiphysin 1 protein being in mammal cells with western blotting analysis, or by measuring the decrease of mRNA of amphiphysin 1 protein being in mammal cells with northern blotting analysis. The composition for restoring a physiological function of senescent cells contains an expression vector having amphiphysin 1 gene of SEQ ID NO:1 as an active ingredient. The physiological function of senescent cells can be restored by introducing the vector having amphiphysin 1 gene into senescent cells of a mammal.

Abstract translation: 目的：提供了用双亲性蛋白1蛋白及其基因检测衰老细胞的方法，用于恢复衰老细胞生理功能的组合物和用于恢复衰老细胞生理功能的方法。 构成：检测哺乳动物细胞中衰老细胞的方法的特征在于用Northern印迹分析测量哺乳动物细胞中两亲素1蛋白的减少，或通过Northern印迹分析测量哺乳动物细胞中两亲素1蛋白mRNA的减少。 用于恢复衰老细胞生理功能的组合物含有具有SEQ ID NO：1的双
磷脂1基因作为活性成分的表达载体。 衰老细胞的生理功能可以通过将具有双亲素1基因的载体导入哺乳动物的衰老细胞而恢复。

公开(公告)号：KR100489188B1

公开(公告)日：2005-05-17

申请号：KR1020000053342

申请日：2000-09-08

Applicant: 재단법인서울대학교산학협력재단

Inventor： 박상철 ,  박웅양 ,  박정수 ,  조경아 ,  김덕인 ,  고영규 ,  서정선

IPC: C12Q1/00

Abstract: 본 발명은 노화 세포의 검출방법, 세포의 노화 유도용 조성물 및 노화 유도 방법에 관한 것으로서, 보다 상세하게는 카베올린을 이용한 노화 세포의 검출방법, 카베올린 유전자가 삽입된 진핵세포용 발현벡터를 포함하는 노화 유도용 조성물 및 이를 이용한 노화 유도 방법에 관한 것으로서, 본 발명의 노화 세포의 검출방법은 노화에 직접적으로 관련된 단백질을 이용함으로써 노화 세포를 보다 정확히 확인할 수 있고, 본 발명의 노화 유도용 조성물은 생체내 노화 유도단백질을 직접적으로 이용하므로 효과적으로 세포를 노화 상태로 유도할 수 있고, 이에 노화 관련 연구, 노화 관련 물질의 연구 및 개발 등에 유용하게 이용할 수 있다.

公开(公告)号：KR1020020020296A

公开(公告)日：2002-03-15

申请号：KR1020000053342

申请日：2000-09-08

Applicant: 재단법인서울대학교산학협력재단

Inventor： 박상철 ,  박웅양 ,  박정수 ,  조경아 ,  김덕인 ,  고영규 ,  서정선

IPC: C12Q1/00

Abstract: PURPOSE: Provided is a method for detecting senescent cells particularly by using caveolin. Also, provided is a composition inducing a senescent state of cells and containing an expression vector for eukaryote into which caveolin gene is inserted. Further, provided is a method for inducing a senescent state of cells by using the composition. CONSTITUTION: The method for detecting senescent cells in mammals is characterized by measuring the increase of caveolin protein being in mammal cells, with western blotting analysis. The composition inducing senescent state of mammal cells contains an expression vector for eukaryote as an active ingredient. The caveolin gene has an amino acid-coding sequence of SEQ ID NO:2. The senescent state of mammal cells is induced by transforming them with the vector containing caveolin gene.

Abstract translation: 目的：提供特别是使用小窝蛋白检测衰老细胞的方法。 此外，提供诱导细胞衰老状态并含有插入窖蛋白基因的真核生物的表达载体的组合物。 此外，提供了通过使用该组合物诱导细胞衰老状态的方法。 构成：检测哺乳动物衰老细胞的方法的特征在于测量哺乳动物细胞中窖蛋白的增加，并通过蛋白质印迹分析。 诱导哺乳动物细胞衰老状态的成分含有真核生物作为活性成分的表达载体。 小窝蛋白基因具有SEQ ID NO：2的氨基酸编码序列。 通过用含有窖蛋白基因的载体转化它们来诱导哺乳动物细胞的衰老状态。

公开(公告)号：KR1020100012714A

公开(公告)日：2010-02-08

申请号：KR1020080074259

申请日：2008-07-29

Applicant: 재단법인서울대학교산학협력재단 ,  국립암센터

Inventor： 박웅양 ,  곽호중 ,  정경채 ,  김수열 ,  채민주

IPC: G01N33/68 ,  G01N33/53 ,  G01N33/48

CPC classification number: G01N33/6872 ,  G01N2500/00

Abstract: PURPOSE: An analysis method for identifying a material which regulates interaction between RNA molecule and protein is provided to simply and quickly identify the material and analyze specificity of sequence of identified compound. CONSTITUTION: An analysis for identifying an agent which controls interaction between ARE sequence fragment and target protein comprises: a step of selecting ARE sequence fragment and probing; a step of preparing a target protein which binds to the ARE sequence fragment and forms complex of ARE sequence fragment and the target protein; a step of detecting formation of the complex; and a step of selecting a candidate based on the complex formation. The ARE sequence fragment contains mRNA sequence of TNF-α, IL-1, IL-2, VEGF, c-myc, c-jun, N-myc, c-fos or Cox 2. The target protein is HuC protein.

Abstract translation: 目的：提供一种用于鉴定调节RNA分子和蛋白质之间的相互作用的材料的分析方法，以简单快速地鉴定材料并分析鉴定化合物序列的特异性。 构成：用于鉴定控制ARE序列片段和靶蛋白之间相互作用的试剂的分析包括：选择ARE序列片段和探测的步骤; 制备与ARE序列片段结合并形成ARE序列片段和靶蛋白质复合物的靶蛋白质的步骤; 检测复合物的形成的步骤; 以及基于复合形成来选择候选的步骤。 ARE序列片段含有TNF-α，IL-1，IL-2，VEGF，c-myc，c-jun，N-myc，c-fos或Cox2的mRNA序列。靶蛋白为HuC蛋白。

公开(公告)号：KR1020020020295A

公开(公告)日：2002-03-15

申请号：KR1020000053341

申请日：2000-09-08

Applicant: 재단법인서울대학교산학협력재단

Inventor： 박상철 ,  박정수 ,  박웅양

IPC: C12Q1/00

Abstract: PURPOSE: Provided are method for detecting senescent cells using amphiphysin 1 protein and its gene, a composition for restoring a physiological function of senescent cells and a method for restoring a physiological function of senescent cells. CONSTITUTION: The method for detecting senescent cells in mammal cells is characterized by measuring the decrease of amphiphysin 1 protein being in mammal cells with western blotting analysis, or by measuring the decrease of mRNA of amphiphysin 1 protein being in mammal cells with northern blotting analysis. The composition for restoring a physiological function of senescent cells contains an expression vector having amphiphysin 1 gene of SEQ ID NO:1 as an active ingredient. The physiological function of senescent cells can be restored by introducing the vector having amphiphysin 1 gene into senescent cells of a mammal.

Abstract translation: 目的：提供使用amphiphysin 1蛋白及其基因检测衰老细胞的方法，用于恢复衰老细胞的生理功能的组合物和恢复衰老细胞的生理功能的方法。 构成：哺乳动物细胞中衰老细胞的检测方法的特征在于通过蛋白质印迹分析法测定哺乳动物细胞中amphiphysin 1蛋白的减少，或者通过Northern印迹分析来测量哺乳动物细胞中amphiphysin 1蛋白的mRNA的减少。 用于恢复衰老细胞的生理功能的组合物含有具有SEQ ID NO：1的两亲肌球蛋白1基因作为活性成分的表达载体。 通过将具有两亲肌肽1基因的载体导入哺乳动物的衰老细胞可以恢复衰老细胞的生理功能。