公开(公告)号：KR1020030086779A

公开(公告)日：2003-11-12

申请号：KR1020020024944

申请日：2002-05-07

Applicant: 재단법인서울대학교산학협력재단

Inventor： 정필훈 ,  장준혁 ,  서병무 ,  정종평

IPC: C07K14/475

CPC classification number: C07K14/47 ,  A61K38/18 ,  C07K14/475

Abstract: PURPOSE: A method for increasing the adhesion of neurons using a tenascin type III 3 fragment is provided, thereby increasing the adhesion of neurons to the artificial nervous conduit used in restoration of neurons. CONSTITUTION: A method for increasing the adhesion of neurons comprises using a polypeptide containing the tenascin type III 3 fragment, wherein the polypeptide has the amino acid sequence of SEQ ID NO: 4 or modification thereof. A method for restoration of neurons comprises using the polypeptide containing the tenascin type III 3 fragment, wherein the polypeptide has the amino acid sequence of SEQ ID NO: 4 or modification thereof. A nervous conduit coated with the polypeptide containing the tenascin type III 3 fragment is provided, wherein the polypeptide has the amino acid sequence of SEQ ID NO: 4 or modification thereof.

Abstract translation: 目的：提供使用腱生蛋白III型3片段增加神经元粘附的方法，从而增加神经元对用于神经元恢复的人造神经导管的粘附。 构成：增加神经元粘附的方法包括使用含有腱生蛋白III型III片段的多肽，其中多肽具有SEQ ID NO：4的氨基酸序列或其修饰。 恢复神经元的方法包括使用含有腱生蛋白III型III片段的多肽，其中多肽具有SEQ ID NO：4的氨基酸序列或其修饰。 提供了包含含有腱生蛋白III型III片段的多肽的神经导管，其中多肽具有SEQ ID NO：4的氨基酸序列或其修饰。

公开(公告)号：KR100869837B1

公开(公告)日：2008-11-21

申请号：KR1020020024944

申请日：2002-05-07

Applicant: 재단법인서울대학교산학협력재단

Inventor： 정필훈 ,  장준혁 ,  서병무 ,  정종평

IPC: C07K14/475

Abstract: 본 발명은 테나신 타입Ⅲ 3 절편을 이용한 신경세포 부착능 향상 방법에 관한 것으로, 보다 상세하게는 테나신 타입Ⅲ 3 절편을 포함하는 폴리펩타이드를 사용하여 신경세포를 부착하는 방법 또는 신경세포를 재생하는 방법 및 상기 테나신 타입Ⅲ 3 절편을 포함하는 폴리펩타이드로 코팅된 신경도관에 관한 것이다. 본 발명의 테나신 타입Ⅲ 3 절편을 포함하는 폴리펩타이드를 이용한 신경세포 부착방법은 신경세포의 증식 또는 재생에 응용할 수 있고, 테나신 타입Ⅲ 3 절편을 포함하는 폴리펩타이드로 코팅된 신경도관은 신경세포 부착능이 향상되어 신경세포 재생능을 향상시킬 수 있다.

公开(公告)号：KR100874184B1

公开(公告)日：2008-12-15

申请号：KR1020020024943

申请日：2002-05-07

Applicant: 재단법인서울대학교산학협력재단

Inventor： 정필훈 ,  장준혁 ,  서병무 ,  정종평

IPC: C07K7/08

Abstract: PURPOSE: An oligopeptide specifically binding to Schwann cells is provided, which is useful for treatment of damage in nerve cells. CONSTITUTION: An oligopeptide specifically binding to Schwann cells is provided, wherein the oligopeptide has the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3. The oligopeptide specifically binding to Schwann cell is isolated by inserting random amino acid sequences into a vector pFLITRIX containing the active portion of thioredoxin; transforming Escherichia coli with the vector pFLITRIX; culturing the transformed E. coli in an ampicillin containing IMC selection medium; adding tryptophane into the medium and culturing it to induce FLITRIX peptide; binding the transformed E. coli to Schwann cells fixed on a dish; selecting peptides specifically binding to the Schwann cells; and sequencing the selected peptides.

Abstract translation: 目的：提供与雪旺细胞特异性结合的寡肽，其可用于治疗神经细胞的损伤。 组成：提供了与雪旺细胞特异性结合的寡肽，其中所述寡肽具有SEQ ID NO：1，SEQ ID NO：2或SEQ ID NO：3的氨基酸序列。通过插入与雪旺细胞特异性结合的寡肽 随机氨基酸序列整合到含有硫氧还蛋白活性部分的载体pFLITRIX中; 用载体pFLITRIX转化大肠杆菌; 在含有氨苄青霉素的IMC选择培养基中培养转化的大肠杆菌; 向培养基中加入色氨酸并培养它以诱导FLITRIX肽; 将转化的大肠杆菌结合到固定在培养皿上的雪旺氏细胞; 选择特异性结合许旺细胞的肽; 并对选定的肽进行测序。

公开(公告)号：KR1020030086778A

公开(公告)日：2003-11-12

申请号：KR1020020024943

申请日：2002-05-07

Applicant: 재단법인서울대학교산학협력재단

Inventor： 정필훈 ,  장준혁 ,  서병무 ,  정종평

IPC: C07K7/08

CPC classification number: C07K7/08 ,  C07K7/06

Abstract: PURPOSE: An oligopeptide specifically binding to Schwann cells is provided, which is useful for treatment of damage in nerve cells. CONSTITUTION: An oligopeptide specifically binding to Schwann cells is provided, wherein the oligopeptide has the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3. The oligopeptide specifically binding to Schwann cell is isolated by inserting random amino acid sequences into a vector pFLITRIX containing the active portion of thioredoxin; transforming Escherichia coli with the vector pFLITRIX; culturing the transformed E. coli in an ampicillin containing IMC selection medium; adding tryptophane into the medium and culturing it to induce FLITRIX peptide; binding the transformed E. coli to Schwann cells fixed on a dish; selecting peptides specifically binding to the Schwann cells; and sequencing the selected peptides.

Abstract translation: 目的：提供特异性结合雪旺氏细胞的寡肽，可用于治疗神经细胞损伤。 构成：提供与雪旺氏细胞特异性结合的寡肽，其中寡肽具有SEQ ID NO：1，SEQ ID NO：2或SEQ ID NO：3的氨基酸序列。通过插入特异性结合雪旺氏细胞的寡肽来分离 将随机氨基酸序列转化成含有硫氧还蛋白活性部分的载体pFLITRIX; 用载体pFLITRIX转化大肠杆菌; 在含有氨苄青霉素的IMC选择培养基中培养转化的大肠杆菌; 将色氨酸加入到培养基中并培养以诱导FLITRIX肽; 将转化的大肠杆菌与固定在盘上的施万细胞结合; 选择与雪旺氏细胞特异性结合的肽; 并对所选择的肽进行测序。