公开(公告)号：KR1020090097503A

公开(公告)日：2009-09-16

申请号：KR1020080022666

申请日：2008-03-11

Applicant: 재단법인서울대학교산학협력재단

Inventor： 박재학 ,  석승혁

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2600/142

Abstract: A method for quantitatively analyzing the absolute value of fluorescence intensity and the amount of EGFP (Enhanced Green Fluorescent Protein) in a transgenic fish is provided to quantitatively measure the harmfulness of toxic material. A method for qualitatively analyzing the amount of GFP or EGFP of transgenic fish comprises: a first step of measuring the fluorescence intensity based on the amount of the GFP and purified GFP and forming standard linear equation; a second step of inserting a toxic material reactive gene promoter in a vector which expresses GFP or EGFP to produce the transgenic fish; a third step of measuring the fluorescence intensity which the transgenic fish expresses; a fourth step of calculating the amount of GFP or EGFP corresponding to the fluorescence intensity; and a step of measuring the amount of purified GFP or EGFP and forming standard linear equation.

Abstract translation: 提供了定量分析转基因鱼的荧光强度绝对值和EGFP（增强型绿色荧光蛋白）量的方法，以定量测定有毒物质的有害性。 用于定性分析转基因鱼的GFP或EGFP的量的方法包括：基于GFP和纯化的GFP的量测量荧光强度并形成标准线性方程的第一步骤; 将毒性物质反应性基因启动子插入到表达GFP或EGFP以产生转基因鱼的载体中的第二步骤; 测量转基因鱼表达的荧光强度的第三步; 计算对应于荧光强度的GFP或EGFP的量的第四步骤; 以及测量纯化的GFP或EGFP的量并形成标准线性方程的步骤。

公开(公告)号：KR1020090097504A

公开(公告)日：2009-09-16

申请号：KR1020080022667

申请日：2008-03-11

Applicant: 재단법인서울대학교산학협력재단

Inventor： 박재학 ,  석승혁

IPC: C12Q1/68 ,  G06F19/28

CPC classification number: G01N33/5014 ,  C12Q1/6827 ,  C12Q2600/142

Abstract: A method for evaluating toxicity through the expression of fluorescence protein in a transgenic embryo of animal is provided to compare toxic degree between different species and analyze at the initial developing stage. A method for evaluating the effect on a toxic material in developing embryo comprises: a first step of injecting a fluorescence protein expression vector in which toxic material-reactive gene promoter is inserted in an embryo to produce a transgenic embryo of animal; and a second step of evaluating the expression of fluorescence protein by exposing the embryo with the toxic material. The animal is zebrafish. The used toxic material is arsenic.

Abstract translation: 提供通过动物转基因胚胎中荧光蛋白表达来评估毒性的方法，以比较不同物种之间的毒性程度，并在初始发育阶段进行分析。 用于评价对发育中的胚胎中的有毒物质的影响的方法包括：将荧光蛋白质表达载体注入第一步骤，其中将有毒物质反应性基因启动子插入胚胎中以产生动物的转基因胚胎; 以及通过用有毒物质暴露胚胎来评估荧光蛋白的表达的第二步骤。 动物是斑马鱼。 使用过的有毒物质是砷。

公开(公告)号：KR1020080009785A

公开(公告)日：2008-01-30

申请号：KR1020060069426

申请日：2006-07-25

Applicant: 재단법인서울대학교산학협력재단

Inventor： 박재학 ,  석승혁

IPC: C12N15/06 ,  C12N15/00

Abstract: A transgenic zebrafish for searching environmental contaminants and a method for preparing the same are provided to evaluate the preclinical effect and sensitivity by providing a biomarker for risk of the environmental contaminants. A method for preparing a transgenic zebrafish for searching environmental contaminants comprises the steps of: (a) cloning a pollutant response gene promoter of human such as HSRE(heat shock responsive element) and AhRE(aryl-hydrocarbon responsive element); (b) inserting the promoter into a vector expressing a fluorescent protein; and (c) introducing the vector into zebrafish. A transgenic zebrafish for searching environmental contaminants prepared by the method is characterized in that it radiates when it is exposed to environmental stress such as heat, cold, oxygen deficiency, Cd, L-azetidine-2-carboxylic acid, 2,3,7,8-tetrachlorodibenzo-p-dioxin, 3-methylcholanthrene, 3,4,5,3',4',5'-hexabromobiphenyl, Aroclor 1254 and benzo[a]pyrene.

Abstract translation: 提供了一种用于检索环境污染物的转基因斑马鱼及其制备方法，通过为环境污染物的风险提供生物标志物来评估临床前效应和敏感性。 制备用于检索环境污染物的转基因斑马鱼的方法包括以下步骤：（a）克隆人的污染物应答基因启动子如HSRE（热休克反应元件）和AhRE（芳烃响应元件）; （b）将启动子插入表达荧光蛋白的载体中; 和（c）将载体引入斑马鱼。 用于搜索通过该方法制备的环境污染物的转基因斑马鱼的特征在于当其暴露于环境胁迫如热，冷，缺氧，Cd，L-氮杂环丁烷-2-羧酸，2,3,7， 8-四氯二苯并对二恶英，3-甲基胆蒽，3,4,5,3'，4'，5'-六溴代二苯，Aroclor 1254和苯并[a]芘。