公开(公告)号：KR1020050022491A

公开(公告)日：2005-03-08

申请号：KR1020030060956

申请日：2003-08-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 이광웅 ,  육희정 ,  김성진

IPC: C12N15/82

CPC classification number: C12N15/8202 ,  C12N15/8257

Abstract: PURPOSE: An ethanol-inducible expression vector is provided, thereby easily obtaining an interest gene from plants at a certain culturing period or stage by using ethanol as an inducing chemical which can be sprayed in a large scale due to its strong volatility. CONSTITUTION: The ethanol-inducible expression vector comprises the nucleotide sequence(SEQ ID NO:1) encoding the ADR1 site which is a transcription factor of Saccharomyces cerevisiae; the nucleotide sequence(SEQ ID NO:2) encoding a promoter of ADH2(alcohol dehydratase 2); and an interest gene operably linked to the promoter, wherein the ADR1 site essentially comprises the nucleotide sequence(SEQ ID NO:3) encoding the TADI site which is the zinc finger site; the ADH2 promoter essentially comprises the nucleotide sequence(SEQ ID NO:4) encoding the UAS site(200bp DNAs); the ADH2 promoter is ADH2-35S fusion promoter having the nucleotide sequence of SEQ ID NO:15; and the alcohol is ethanol.

Abstract translation: 目的：提供乙醇诱导表达载体，从而通过使用乙醇作为诱导化学品，在一定的培养期或阶段容易地从植物获得感兴趣的基因，由于其强烈的挥发性而能够大规模喷洒。 构成：乙醇诱导型表达载体包含编码作为酿酒酵母转录因子的ADR1位点的核苷酸序列（SEQ ID NO：1） 编码ADH2启动子（醇脱水酶2）的核苷酸序列（SEQ ID NO：2）; 和与启动子可操作地连接的兴趣基因，其中ADR1位点基本上包含编码作为锌指位点的TADI位点的核苷酸序列（SEQ ID NO：3） ADH2启动子基本上包含编码UAS位点（200bp DNA）的核苷酸序列（SEQ ID NO：4）。 ADH2启动子是具有SEQ ID NO：15的核苷酸序列的ADH2-35S融合启动子; 酒精是乙醇。