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公开(公告)号：KR1020080018617A
公开(公告)日：2008-02-28
申请号：KR1020060081020
申请日：2006-08-25
Applicant: 재단법인서울대학교산학협력재단
Abstract: A multipotential cell line I derived from cancer tissue is provided to treat various diseases by differentiating into various types of cells including epidermal cell and nerve cell, study on the cancer development mechanism, and develop a new anticancer agent targeting proteins expressed from the same cell line. A multipotential cell line I(KCLRF-BP-00137) is derived from cancer tissue, can be cultured in a suspension state, and expresses markers of breast stem cell(CK19, CK5, CK6), a marker of breast cancer resistant drug(ABCG2), a marker of breast cancer diagnosis receptor(C-erBb2), markers of epidermal cell(CK14, vimentin, CK18) and markers of nerve cell(nestin, Tuj-1). A method for preparing the multipotential cell line I(KCLRF-BP-00137) comprises the steps of: (1) separating cancer cells from the previously separated cancer tissue; (2) culturing the separated cancer cells in a suspension medium; (3) collecting the cells in a suspension state from the cultured medium; and (4) subculturing the collected cells in a suspension medium to separate the multipotential cell line. Further, the cancer tissue is breast cancer.
Abstract translation: 提供衍生自癌组织的多能细胞系I，通过分化成包括表皮细胞和神经细胞在内的各种细胞，研究癌症发展机制，开发一种靶向相同细胞系表达的蛋白质的新型抗癌剂来治疗各种疾病 。 多能细胞系I（KCLRF-BP-00137）衍生自癌组织，可以以悬浮状态进行培养，并表达乳腺癌细胞（CK19，CK5，CK6），乳腺癌抗药物（ABCG2 ），乳腺癌诊断受体（C-erBb2）的标志物，表皮细胞（CK14，波形蛋白，CK18）的标记物和神经细胞标记物（巢蛋白，Tuj-1）。 制备多潜能细胞系I（KCLRF-BP-00137）的方法包括以下步骤：（1）从先前分离的癌组织分离癌细胞; （2）在悬浮介质中培养分离的癌细胞; （3）从培养基中收集处于悬浮状态的细胞; 和（4）将收集的细胞传代培养在悬浮介质中以分离多潜能细胞系。 此外，癌组织是乳腺癌。
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公开(公告)号：KR100868316B1
公开(公告)日：2008-11-11
申请号：KR1020050032872
申请日：2005-04-20
Applicant: 재단법인서울대학교산학협력재단
Abstract: 본 발명은 유방 조직에서 유래된 다능성 줄기 세포 및 (a) 유방 조직으로부터 유방 세포를 분리하는 단계; (b) 상기 유방 세포를 줄기 세포 배양용 배지에서 일차 배양하는 단계; (c) 상기 일차 배양물로부터 부유 상태의 세포들을 수집하는 단계; 및 (d) 상기 부유 상태의 세포들을 줄기 세포 배양용 배지에서 계대 배양하여 다능성 줄기 세포를 분리하는 단계를 포함한 유방 조직 유래의 다능성 줄기 세포의 제조방법에 관한 것이다.
또한, 본 발명은 상기 유방 조직 유래의 다능성 줄기 세포로부터 분화된 세포 및 (a) 유방 조직 유래의 다능성 줄기 세포를 목적 분화 세포 배양용 배지에서 배양하는 단계 및 (b) 배양 중에 목적 분화 세포의 마커를 발현하는 분화 세포를 분리하는 단계를 포함한 유방 조직 유래의 다능성 줄기 세포의 제조방법에 관한 것이다. 본 발명에 따른 유방 조직 유래의 다능성 줄기 세포는 상피 세포, 신경 세포 또는 골수 세포를 비롯하여 인체를 구성하는 여러 유형의 세포로 분화할 수 있다.-
公开(公告)号：KR1020080018619A
公开(公告)日：2008-02-28
申请号：KR1020060081022
申请日：2006-08-25
Applicant: 재단법인서울대학교산학협력재단
Abstract: A multipotential cell line II derived from cancer tissue is provided to differentiate into other cells such as epidermal cell and nerve cell except cells in the cancer tissue, and separate cancer stem cell, so that it is useful for treatment of various diseases. A method for preparing the multipotential cell line II(KCLRF-BP-00138) comprises the steps of: (1) separating cancer cells from the previously separated cancer tissue; (2) culturing the separated cancer cells in a suspension medium; (3) collecting the cells in a suspension state from the cultured medium; (4) subculturing the collected cells in a suspension medium to separate the multipotential cell line; and (5) optionally culturing the separated multipotential cell line in medium for adhesion. A multipotential cell line II(KCLRF-BP-00138) derived from cancer tissue and being cultured in a suspension state does not express markers of breast cancer diagnosis receptor(ER, PR, C-erBb2), and express markers of breast stem cell(CK19, CK5, CK6), a marker of breast cancer resistant drug(ABCG2), markers of epidermal cell(CK14, vimentin, CK18) and markers of nerve cell(nestin, Tuj-1). Further, the cancer tissue is breast cancer.
Abstract translation: 提供衍生自癌组织的多能细胞系II，以分化成其他细胞，如癌组织中除细胞外的表皮细胞和神经细胞，以及分离的癌干细胞，因此可用于治疗各种疾病。 制备多潜能细胞系II（KCLRF-BP-00138）的方法包括以下步骤：（1）从先前分离的癌组织分离癌细胞; （2）在悬浮介质中培养分离的癌细胞; （3）从培养基中收集处于悬浮状态的细胞; （4）将收集的细胞在悬浮培养基中传代培养以分离多潜能细胞系; 和（5）任选地在培养基中培养分离的多能细胞系以进行粘附。 衍生自癌组织并以悬浮状态培养的多能细胞系II（KCLRF-BP-00138）不表达乳腺癌诊断受体（ER，PR，C-erBb2）的标志物，并且表达乳腺干细胞的标志物 CK19，CK5，CK6），乳腺癌抗药物标记物（ABCG2），表皮细胞标志物（CK14，波形蛋白，CK18）和神经细胞标记物（nestin，Tuj-1）。 此外，癌组织是乳腺癌。
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公开(公告)号：KR1020060110542A
公开(公告)日：2006-10-25
申请号：KR1020050032872
申请日：2005-04-20
Applicant: 재단법인서울대학교산학협력재단
Abstract: Stem cells derived from the normal breast tissue and breast cancer tissue, a preparation method thereof and differentiated cells from the same stem cells are provided to be differentiated into various cells in human body, so that various diseases are treated. The multi-functional stem cells derived from the normal breast tissue and breast cancer tissue are provided, wherein the stem cells are capable of being differentiated into epidermal cells, nerve cells or bone marrow cells. The method for preparing the multi-functional stem cells derived from the breast tissue comprises the steps of: (a) separating breast cells from the breast tissue; (b) culturing the breast cells in a medium; (c) collecting the floating breast cells from the cultured medium; and (d) subculturing the floating breast cells in a medium and separating the multi-functional stem cells. The differentiated cells are prepared by (a) culturing the multi-functional stem cells derived from the breast tissue in a medium, and (b) separating the target differentiated cells expressing a marker during the cultivation, wherein the target differentiated cell is an epidermal cell, nerve cell or bone marrow cell.
Abstract translation: 提供衍生自正常乳腺组织和乳腺癌组织的干细胞，其制备方法和来自相同干细胞的分化细胞以分化成人体中的各种细胞，从而治疗各种疾病。 提供衍生自正常乳腺组织和乳腺癌组织的多功能干细胞，其中干细胞能够分化为表皮细胞，神经细胞或骨髓细胞。 从乳房组织制备多功能干细胞的方法包括以下步骤：（a）从乳房组织分离乳腺细胞; （b）在培养基中培养乳腺细胞; （c）从培养基中收集浮动的乳腺细胞; 和（d）将介质中的浮动乳腺细胞传代培养并分离多功能干细胞。 分化细胞通过（a）在培养基中培养衍生自乳腺组织的多功能干细胞，和（b）在培养期间分离表达标志物的靶分化细胞，其中靶分化细胞是表皮细胞 ，神经细胞或骨髓细胞。
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