公开(公告)号：KR1020040020226A

公开(公告)日：2004-03-09

申请号：KR1020020051754

申请日：2002-08-30

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  장덕진

IPC: A61K31/327

Abstract: PURPOSE: A K ion channel mediator of a sensory neuron of aplysia containing hydrogen peroxide is provided. A sensory neuron system of aplysia using the K ion channel mediator can be provided as a model for the study of memory and learning. CONSTITUTION: The mediator for K ion channel of a sensory neuron of aplysia contains hydrogen peroxide. In the K ion channel, a hydrogen-induced depolarization is inhibited by tetraethylammonium(TEA). The concentration of hydrogen peroxide in the range of 0.01 to 10mK induces hyperpolarization within 1hr and contributes to the increase of membrane conductance. The aplysia is an Aplysia kurodai captured in a costal area of The East Sea of Korea.

Abstract translation: 目的：提供含有过氧化氢的含有aplysia的感觉神经元的K离子通道介体。 可以提供使用K离子通道介质的感觉神经元系统，作为记忆和学习研究的模型。 构成：aplysia感觉神经元K离子通道的介质含有过氧化氢。 在K离子通道中，氢诱导的去极化被四乙基铵（TEA）抑制。 0.01〜10mK范围内的过氧化氢浓度在1小时内引起超极化，有助于膜电导的增加。 大赦国际是在韩国东海沿海地区捕获的阿派利亚kurodai。

公开(公告)号：KR1020040019613A

公开(公告)日：2004-03-06

申请号：KR1020020051211

申请日：2002-08-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  장덕진

IPC: C12N15/12

CPC classification number: C07K14/43504 ,  C12N5/0619 ,  C12N15/85 ,  C12N15/89

Abstract: PURPOSE: An octopamine receptor isolated from Aplysia kurodai and an activation method of PKA(cAMP-dependent protein kinase) pathway are provided, thereby improving short-period memory and screening a drug activating the PKA pathway to improve memory. CONSTITUTION: An octopamine receptor gene(Ap oa1) isolated from Aplysia kurodai encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO: 21, wherein the octopamine receptor gene(Ap oa1) has the nucleotide sequence set forth in SEQ ID NO: 20. A neuron expression system comprises (a) a vector containing a membrane receptor gene; and (b) a vector-microinjected neuron, wherein the membrane receptor gene encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO: 21; and the membrane receptor gene has the nucleotide sequence set forth in SEQ ID NO: 20; the vector is pNEXδ-Ap oa1 or pNEX3-Ap oa1-HA. An activation method of PKA pathway comprises (a) transduction of a vector containing the octopamine receptor gene into the neuron; (b) selecting a neuron expressing the octopamine receptor; and (c) treating the selected neuron with octopamine.

Abstract translation: 目的：提供从黑腹苷酸分离的章鱼胺受体和PKA（cAMP依赖性蛋白激酶）途径的活化方法，从而改善短期记忆和筛选激活PKA途径的药物以改善记忆。 构成：从黑腹果蝇分离的章鱼胺受体基因（Apo1）编码具有SEQ ID NO：21所示的氨基酸序列的多肽，其中所述章鱼胺受体基因（Apoa1）具有SEQ ID NO 20.神经元表达系统包含（a）含有受体基因的载体; 和（b）载体微注射神经元，其中所述膜受体基因编码具有SEQ ID NO：21所示氨基酸序列的多肽; 膜受体基因具有SEQ ID NO：20所示的核苷酸序列; 载体是pNEXδ-Apoa1或pNEX3-Apoa1-HA。 PKA途径的活化方法包括（a）将含有章鱼胺受体基因的载体转导入神经元; （b）选择表达章鱼胺受体的神经元; 和（c）用章鱼胺处理所选择的神经元。