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    페록시좀 증식제를 선별할 수 있는 DNA 칩
    2.
    发明公开
    페록시좀 증식제를 선별할 수 있는 DNA 칩 有权
    用于筛选过氧化物酶体增殖物的DNA芯片

    公开(公告)号：KR1020070011417A

    公开(公告)日：2007-01-24

    申请号：KR1020067022611

    申请日：2004-04-13

    Applicant: (주)지노첵 ,  재단법인서울대학교산학협력재단 ,  주식회사 이즈텍 ,  (주)신원사이언스

    Inventor： 황승용 ,  정진욱 ,  오문주 ,  김승준 ,  연종필 ,  김준섭 ,  김용현 ,  이창현 ,  윤현규 ,  염혜정 ,  강경선 ,  이영순 ,  박준석 ,  황재웅 ,  김양석 ,  이완선 ,  전기선 ,  엄찬휘 ,  강종수 ,  이경재 ,  송복수 ,  전금진 ,  여찬동

    IPC: C12Q1/68

    CPC classification number: C12Q1/6883 ,  C12Q2600/158 ,  C12Q1/6837

    Abstract: A DNA chip and a kit for screening substances showing activity as a peroxisome proliferator are provided to be able to rapidly and accurately screen the peroxisome proliferator inducing abnormal cell reaction in vivo, thereby being widely applied to safety test of various materials. The DNA chip for screening of peroxisome proliferator comprises: a probe consisting of 121 to 587 nucleotides, capable of binding specifically to a gene whose expression is induced by a peroxisome proliferators; a linker including oligo (dT)15, -(CH2)6- and amine group in sequence, whose 3'-terminal of oligo (dT)15 is linked to 5'-terminal of the probe; and a solid substrate, whose surface is coated with aldehyde group, connecting with amine group of the linker via Schiff's base reaction. In the DNA chip, the probe is at least one gene selected from SEQ ID Nos: 1 to 12. The kit for screening of peroxisome proliferator comprises the DNA chip and a fluorescence-labeled deoxynucleotide to be used for labeling cDNA synthesized from RNA isolated from a sample. The method for screening peroxisome proliferator using the kit comprises the steps of: (a) administering a peroxisome proliferator candidate chemical to an animal; (b) isolating RNA from the animal; (c) synthesizing fluorescence-labeled cDNA from the isolated RNA using fluorescence-labeled deoxynucleotide in the kit; (d) applying the synthesized cDNA to a DNA chip for screening peroxisome proliferator in the kit to allow hybridization; and (e) after washing the hybridized DNA chip, examining the hybridized probe in the DNA chip by using a scanner.

    Abstract translation: 提供了用于筛选显示出作为过氧化物酶体增殖物活性的物质的DNA芯片和试剂盒，以能够快速且准确地筛选在体内诱导异常细胞反应的过氧化物酶体增殖物，从而广泛应用于各种材料的安全性试验。 用于筛选过氧化物酶体增殖物的DNA芯片包括：由121至587个核苷酸组成的探针，能够特异性结合其表达由过氧化物酶体增殖物诱导的基因; 包含寡核苷酸（dT）15， - （CH2）6-和胺基的接头，其寡核苷酸（dT）15的3'-末端连接到探针的5'-末端; 和表面用醛基涂覆的固体基质，通过Schiff碱反应与连接体的胺基连接。 在DNA芯片中，探针是选自SEQ ID NO：1〜12的至少一种基因。用于筛选过氧化物酶体增殖物的试剂盒包含DNA芯片和荧光标记的脱氧核苷酸，用于标记从分离自 一个样品。 使用试剂盒筛选过氧化物酶体增殖物的方法包括以下步骤：（a）向动物施用过氧化物酶体增殖物候选化学品; （b）从动物中分离RNA; （c）使用试剂盒中荧光标记的脱氧核苷酸从分离的RNA合成荧光标记的cDNA; （d）将合成的cDNA应用于DNA芯片，用于筛选试剂盒中的过氧化物酶体增殖物以进行杂交; 和（e）在洗涤杂交的DNA芯片之后，通过使用扫描仪检查DNA芯片中的杂交探针。

    신규한 환경 스트레스 저항성 전사인자 및 이를 이용하여식물체의 환경 스트레스 저항성을 증가시키는 방법
    6.
    发明公开
    신규한 환경 스트레스 저항성 전사인자 및 이를 이용하여식물체의 환경 스트레스 저항성을 증가시키는 방법 失效
    新型环境耐压转录因子及其使用方法增强植物的环境耐力

    公开(公告)号：KR1020060132442A

    公开(公告)日：2006-12-21

    申请号：KR1020060043408

    申请日：2006-05-15

    Applicant: 재단법인서울대학교산학협력재단

    Inventor： 김민균 ,  정진욱 ,  원소윤

    IPC: C07K14/415 ,  C12N15/29

    CPC classification number: C07K14/415 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12N15/8281

    Abstract: A novel environmental stress resistance transcription factor is provided to be able to induce the expression of various environmental stress resistance related genes, thereby being effective for inhibiting or delaying growth decrease of plants or productivity decrease caused by the environmental stress. The environmental stress resistance transcription factor protein includes an amino acid sequence described as SEQ ID : NO. 35 and consists of an amino acid sequence described as SEQ ID : NO. 4, wherein the environmental stress is selected from group consisting of pathogenic bacteria, highly concentrated salt, low temperature or stress caused by oxidation. The method for increasing the environmental stress resistance of a plant is characterized in that it comprises a step of inserting a transcription factor gene encoding the transcription factor protein into a plant expression vector and then introducing it into the plant.

    Abstract translation: 提供了一种新的环境胁迫抗性转录因子，以能够诱导各种与环境胁迫相关的基因的表达，从而有效抑制或延缓植物的生长减少或由环境胁迫引起的生产力降低。 环境胁迫抗性转录因子蛋白包括SEQ ID NO：1所示的氨基酸序列。 35并由如SEQ ID NO：1所示的氨基酸序列组成。 4，其中环境应力选自病原菌，高浓度盐，低温或由氧化引起的应力。 提高植物的环境胁迫抗性的方法的特征在于其包括将编码转录因子蛋白的转录因子基因插入植物表达载体中，然后将其引入植物的步骤。

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