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公开(公告)号：KR1020070014964A
公开(公告)日：2007-02-01
申请号：KR1020060058841
申请日：2006-06-28
Applicant: 재단법인서울대학교산학협력재단
CPC classification number: G01N21/658 , B82Y15/00 , G01N33/532 , G01N33/54346
Abstract: A surface-enhanced Raman scattering nano-tagging particle and a preparation method thereof are provided to reduce the preparation costs of the particle by using silica, and to detect biological molecules economically and rapidly. The surface-enhanced Raman scattering nano-tagging particle comprises a silica core particle having a nano silver particle immobilized with a tagging material and a silica skin precursor in the surface, and a silica skin enclosing the silica core particle, wherein the size of the silica core particle is 50-300nm. The tagging material is 2-methylbenzenethiol, 4-methylbenzenethiol, 4-mercaptopyridine, 2-naphthalenethiol, 4-methoxybenzenethiol, 3-methoxybenzenethiol, 3,4-dimethylbenzenethiol, thiophenol or 3,5-dimethylbenzenethiol, and the silica skin precursor is 3-mercaptopropyltrimethoxysilane or 3-aminopropyltriethoxysilane. The silica skin is formed by tetraethyl orthsilicate and sodium silicate and has the thickness of 10-50 nm. The method for preparing the surface-enhanced Raman scattering nano-tagging particle comprises the steps of: introducing nano silver particle on the surface of silica core particle; immobilizing the tagging material and silica skin precursor to the nano silver particle; and forming the silica skin around the silica core particle immobilized with the tagging material and silica skin precursor.
Abstract translation: 提供表面增强拉曼散射纳米标记颗粒及其制备方法，以通过使用二氧化硅降低颗粒的制备成本，并且经济和快速地检测生物分子。 表面增强拉曼散射纳米标签颗粒包括具有固定有标签材料的纳米银颗粒和表面上的二氧化硅表皮前体的二氧化硅芯颗粒和包围二氧化硅芯颗粒的二氧化硅皮，其中二氧化硅 核心颗粒为50-300nm。 标记材料是2-甲基苯硫酚，4-甲基苯硫醇，4-巯基吡啶，2-萘硫醇，4-甲氧基苯硫醇，3-甲氧基苯硫醇，3,4-二甲基苯硫醇，苯硫酚或3,5-二甲基苯硫酚，二氧化硅皮前体为3- 巯基丙基三甲氧基硅烷或3-氨基丙基三乙氧基硅烷。 二氧化硅皮由四乙基硅酸盐和硅酸钠形成，厚度为10-50nm。 制备表面增强拉曼散射纳米标记颗粒的方法包括以下步骤：将纳米银颗粒引入二氧化硅核颗粒表面; 将标签材料和二氧化硅表皮前体固定在纳米银颗粒上; 以及在固定有标记材料和二氧化硅皮前体的二氧化硅核心颗粒周围形成二氧化硅皮肤。
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公开(公告)号：KR100733085B1
公开(公告)日：2007-06-28
申请号：KR1020060058841
申请日：2006-06-28
Applicant: 재단법인서울대학교산학협력재단
CPC classification number: G01N21/658 , B82Y15/00
Abstract: 본 발명은 생체분자 고속 검출에 사용될 수 있는 표면 증강 라만 산란 나노-표지 입자 및 이의 제조방법에 관한 것이다. 본 발명에 따른 표면증강 라만 산란 나노-표지 입자는 매우 간편하게 제조될 수 있으며, 라만 스펙트럼에 매우 민감하기 때문에 분석이 용이하다는 이점이 있다. 또한, 표면에 표지될 수 있는 화학물질로 다양한 종류가 사용될 수 있기 때문에, 매우 경제적으로 제조될 수 있다. EK라서, 본 발명에 따른 표면증강 라만 산란 나노-표지 입자는 생체분자를 검출하는 데 매우 유용하게 사용될 수 있다.
실리카 입자, 은나노 입자, 실리카 껍질, 라만 스펙트럼-
公开(公告)号：KR100650522B1
公开(公告)日：2006-11-27
申请号：KR1020050086275
申请日：2005-09-15
Applicant: 재단법인서울대학교산학협력재단
IPC: G01N33/50
Abstract: A method for selecting and analyzing compounds or biomolecules is provided to more economically screen many compounds and biomolecules with high speed by using microbead labeled by surface-enhanced Raman scattering and dielectrophoresis. The method comprises the steps of: (a) labelling micro-beads with nano-silver particles and a chemical material showing high binding force with the nano-silver particles such as 2-methylbenzene thiol, 4-methylbenzene thiol, 2-naphthalene thiol, 4-methoxybenzene thiol, 3-methoxybenzene thiol, 3,4-dimethylbenzene thiol, 3,5-dimethylbenzene thiol, 2-mercapto toluene, 4-mercapto toluene and 4-mercapto pyridine; (b) introducing a biomolecule-specific ligand such as biotin, antibody, lectin and peptide into the labelled micro-bead surface; (c) introducing a biomolecule into the ligand-introduced microbead; (d) selecting the biomolecule-introduced microbead using dielectrophoresis; and (e) analyzing the selected biomolecule through surface-enhanced raman spectroscopy.
Abstract translation: 提供了一种选择和分析化合物或生物分子的方法，通过使用表面增强拉曼散射和介电电泳标记的微珠，更经济地筛选许多化合物和生物分子。 该方法包括以下步骤：（a）用纳米银颗粒和与纳米银颗粒显示出高结合力的化学物质例如2-甲基苯硫醇，4-甲基苯硫醇，2-萘硫醇， 4-甲氧基苯硫醇，3-甲氧基苯硫醇，3,4-二甲基苯硫醇，3,5-二甲基苯硫醇，2-巯基甲苯，4-巯基甲苯和4-巯基吡啶; （b）将生物素，抗体，凝集素和肽等生物分子特异性配体导入标记的微珠表面; （c）将生物分子引入配体引入的微珠中; （d）使用介电电泳选择导入生物分子的微珠; 和（e）通过表面增强拉曼光谱分析选定的生物分子。
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