웨스턴 블럿팅 및 면역침강에 이용 가능한 셀레노프로테인 O의 항체 및 그 제조방법
    1.
    发明公开
    웨스턴 블럿팅 및 면역침강에 이용 가능한 셀레노프로테인 O의 항체 및 그 제조방법 有权
    针对西班牙黑斑病和免疫应答的抗白蛋白O抗体及其制备方法

    公开(公告)号:KR1020100098039A

    公开(公告)日:2010-09-06

    申请号:KR1020090017002

    申请日:2009-02-27

    Abstract: PURPOSE: An antibody to a specific region of selenoprotein O is provided to use in western blotting and immunoprecipitation and to quantify and qualify selenoprotein O. CONSTITUTION: An isolated peptide comprises an amino acid sequence of sequence number 1. The peptide contains a cystein at N-terminal for KLH conjugation. The peptide is modified by amidation of C-terminal. An antibody which specifically binds to the peptide with an amino acid of sequence number 1 specifically binds to selenoprotein O. The antibody is used in western blotting or ELISA analysis. A method for detecting a specific polypeptide in a sample comprises: a step of contacting the antibody to the sample; a step of forming complex of the specific polypeptide and antibody; and a step of detecting the complex.

    Abstract translation: 目的:提供用于蛋白质印迹和免疫沉淀的特定区域的抗体,并对硒蛋白O进行定量和鉴定。构成:分离的肽包含序列号1的氨基酸序列。肽含有N末端的半胱氨酸 - KLH缀合的末端。 肽通过C-末端的酰胺化来修饰。 与序列号1的氨基酸特异性结合肽的抗体特异性结合于硒蛋白O.抗体用于western印迹或ELISA分析。 用于检测样品中特定多肽的方法包括:使抗体与样品接触的步骤; 形成特异性多肽和抗体复合物的步骤; 以及检测复合物的步骤。

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