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公开(公告)号：KR101116872B1
公开(公告)日：2012-03-06
申请号：KR1020110124402
申请日：2011-11-25
Applicant: 전남대학교산학협력단
IPC: C07K7/08 , C07K14/47 , C07K16/18 , A61K39/395
Abstract: 본발명은면역원성이증가된펩티드항원및 이를생산하는방법을제공한다. 또한본 발명은면역원성이증가된펩티드항원, 이를코딩하는핵산또는본 발명의펩티드항원에대한항체를포함하는백신조성물및 이를생산하는방법을제공한다. 본발명은또한목표항원에대한항체를보다효율적으로생산하는방법에관한것이다. 보다상세하게는, 본발명은목표항원(들)의에피토프영역이일렬반복(tandem repeat)된폴리펩티드또는상기에피토프영역이일렬반복된서열사이에프롤린이도입된폴리펩티드및 이를이용하는방법을제공한다. 본발명의에피토프영역이직렬반복된폴리펩티드는모노-에피토프펩티드보다면역원성이더 높으며, 프롤린이도입될경우면역원성이더 높아져항체생산효율이더욱증가한다.
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公开(公告)号：KR101018878B1
公开(公告)日：2011-03-04
申请号：KR1020090011206
申请日：2009-02-11
Applicant: 전남대학교산학협력단
Abstract: 본 발명은 대장균에서 발현된 내포체 (inclusion body)로부터 재조합 Bax 억제제-1 (BI-1)의 동시 리폴딩 및 재구성에 관한 것이다. 기능적 분석은 결과적으로 수득되는 프로테오리포좀 (proteoliposome)이 산성 환경에 반응하고 리포좀으로부터 포접된 Ca
2+ 의 방출을 유발한다는 것을 보여주었다. 원편광 이색성 분광분석 (Circular Dichoism)에 의해 결정된, 재구성된 BI-1의 이차 구조는 산성 환경에 노출시 α-헬릭스 함량의 증가 및 랜덤 구조의 감소를 입증했다. 이 구조적 변화가 BI-1의 양성자-유도 Ca
2+ 방출을 초래하는 것일 수 있다.
Bax 억제제-1, 리포좀, 리폴딩, 재구성.-
公开(公告)号：KR1020100097526A
公开(公告)日：2010-09-03
申请号：KR1020090016511
申请日：2009-02-26
Applicant: 전남대학교산학협력단
IPC: C07K16/18 , A61K39/395
Abstract: PURPOSE: A peptide antigen with enhanced immunogenicity and a method for producing the same are provided to enhance immunogenicity and to increase antibody production efficiency. CONSTITUTION: A peptide antigen with enhanced immunogenicity comprises an amino acid sequence with two or more tandem repeat amino acid sequence unit. The peptide antigen additionally contains proline between amino acid sequence units. A vaccine composition contains the peptide antigen, nucleic acid encoding the same, or antibody to the peptide antigen. A method for producing the peptide antigen with immunogenicity comprises: a step of determining amino acid sequence corresponding to an epitope of a target antigen; and a step of preparing a polypeptide comprising two or more tandem repeat amino acid sequence units; or a step of preparing amino acid sequence which additionally contains proline.
Abstract translation: 目的：提供具有增强的免疫原性的肽抗原及其制备方法，以增强免疫原性并提高抗体生产效率。 构成：具有增强的免疫原性的肽抗原包含具有两个或多个串联重复氨基酸序列单元的氨基酸序列。 肽抗原在氨基酸序列单元之间另外含有脯氨酸。 疫苗组合物含有肽抗原，编码其的核酸或针对肽抗原的抗体。 用于产生具有免疫原性的肽抗原的方法包括：确定对应于靶抗原表位的氨基酸序列的步骤; 以及制备包含两个或多个串联重复氨基酸序列单元的多肽的步骤; 或另外含有脯氨酸的氨基酸序列的制备工序。
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Patent Agency Ranking
公开(公告)号：KR101654313B1
公开(公告)日：2016-09-22
申请号：KR1020140134817
申请日：2014-10-07
Applicant: 전남대학교산학협력단
Abstract: 본발명은시토크롬 P450(CYP)의정제방법을제공한다. 본발명에따르면대표적인단백질정제방법인친화성-태그및 크로마토그래피등을이용하지않고, 혼합미셀을이용하여원-스텝방식으로시토크롬 P450을간편하고신속하게고효율및 고순도로정제할수 있다. 본발명은혼합미셀및 아비딘-비드를포함하는시토크롬 P450(CYP) 정제용조성물을제공한다.
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5.发明公开대장균에서 발현된 내포체로부터 사람 재조합 Ｂａｘ억제제-1의 리폴딩 및 리포좀으로의 재구성 有权人类重组BAX抑制剂-1在ESCHERICHIA COLI中表达的包含体的脂质体的重构和重构
公开(公告)号：KR1020100090172A
公开(公告)日：2010-08-13
申请号：KR1020090011206
申请日：2009-02-11
Applicant: 전남대학교산학협력단
Abstract: PURPOSE: A method for refolding and recombining recombinant Bas inhibitor-1(BI-1) is provided to use for in vitro functional analysis of BI-1 and to induce Ca^2+ release. CONSTITUTION: A method for producing a liposome containing BI-1 protein comprises: a step of separating an inclusion body containing BI-1 protein; a step of dissolving charotropic agent of first concentration, reductant, and amphoteric surfactant in a first buffer; a step of mixing the first buffer with phospholipid; and a step of exchanging the first buffer to a second buffer. The charotropic agent is urea or guanidiniumchloride.
Abstract translation: 目的：提供重组和重组巴斯抑制剂-1（BI-1）的方法用于BI-1的体外功能分析和诱导Ca 2+释放。 构成：含有BI-1蛋白质的脂质体的制造方法包括：分离含有BI-1蛋白质的包涵体的工序; 将第一浓度，还原剂和两性表面活性剂的二性反应剂溶解在第一缓冲液中的步骤; 将第一缓冲液与磷脂混合的步骤; 以及将第一缓冲器交换到第二缓冲器的步骤。 不合适剂是尿素或氯化胍。
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公开(公告)号：KR1020160041245A
公开(公告)日：2016-04-18
申请号：KR1020140134817
申请日：2014-10-07
Applicant: 전남대학교산학협력단
Abstract: 본발명은시토크롬 P450(CYP)의정제방법을제공한다. 본발명에따르면대표적인단백질정제방법인친화성-태그및 크로마토그래피등을이용하지않고, 혼합미셀을이용하여원-스텝방식으로시토크롬 P450을간편하고신속하게고효율및 고순도로정제할수 있다. 본발명은혼합미셀및 아비딘-비드를포함하는시토크롬 P450(CYP) 정제용조성물을제공한다.
Abstract translation: 提供细胞色素P450（CYP）的纯化方法。 根据本发明，CYP可以通过使用混合胶束的一步法以高效率和高纯度简单快速地纯化，而不使用典型的蛋白质纯化方法如亲和标记，色谱等。在本发明中，提供 是包含用于纯化CYP的混合胶束和抗生物素蛋白珠的组合物。
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公开(公告)号：KR1020110134358A
公开(公告)日：2011-12-14
申请号：KR1020110124402
申请日：2011-11-25
Applicant: 전남대학교산학협력단
IPC: C07K7/08 , C07K14/47 , C07K16/18 , A61K39/395
Abstract: PURPOSE: A method for preparing a peptide antigen with enhanced immunogenicity is provided to enhance antibody-producing efficiency. CONSTITUTION: A polypeptide contains an amino acid sequence with two or more tandem repeat LEDTQKH amino active sequences and additionally contains one or two praline between the repeat amino acid sequences. The polypeptide is linked to polystyrene or polylactic-glycolic acid polymers or bovine serum albumin(BSA) or ovalbumin. A vaccine composition contains: the polypeptide with two or more tandem repeat LEDTQKH amino active sequences; the polypeptide with LEDTQKH sequences and proline; the polypeptide linked by the polystyrene or polylactic-glycolic acid polymers or bovine serum albumin(BSA) or ovalbumin; a nucleic acid encoding one of polypeptides; and an antibody for one of the polypeptides.
Abstract translation: 目的：提供增强免疫原性制备肽抗原的方法，以提高抗体产生效率。 构成：多肽含有具有两个或多个串联重复LEDTQKH氨基活性序列的氨基酸序列，并且在重复氨基酸序列之间另外含有一个或两个脯氨酸。 多肽与聚苯乙烯或聚乳酸 - 乙醇酸聚合物或牛血清白蛋白（BSA）或卵清蛋白连接。 疫苗组合物包含：具有两个或更多个串联重复的LEDTQKH氨基活性序列的多肽; 该多肽与LEDTQKH序列和脯氨酸; 通过聚苯乙烯或聚乳酸 - 乙醇酸聚合物或牛血清白蛋白（BSA）或卵清蛋白连接的多肽; 编码多肽之一的核酸; 和一种多肽的抗体。
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公开(公告)号：KR101147476B1
公开(公告)日：2012-05-25
申请号：KR1020090016511
申请日：2009-02-26
Applicant: 전남대학교산학협력단
IPC: C07K16/18 , A61K39/395
Abstract: PURPOSE: A peptide antigen with enhanced immunogenicity and a method for producing the same are provided to enhance immunogenicity and to increase antibody production efficiency. CONSTITUTION: A peptide antigen with enhanced immunogenicity comprises an amino acid sequence with two or more tandem repeat amino acid sequence unit. The peptide antigen additionally contains proline between amino acid sequence units. A vaccine composition contains the peptide antigen, nucleic acid encoding the same, or antibody to the peptide antigen. A method for producing the peptide antigen with immunogenicity comprises: a step of determining amino acid sequence corresponding to an epitope of a target antigen; and a step of preparing a polypeptide comprising two or more tandem repeat amino acid sequence units; or a step of preparing amino acid sequence which additionally contains proline.
Abstract translation: 目的：提供具有增强的免疫原性的肽抗原及其生产方法，以增强免疫原性并增加抗体生产效率。 构成：具有增强的免疫原性的肽抗原包含具有两个或更多个串联重复氨基酸序列单元的氨基酸序列。 肽抗原另外在氨基酸序列单元之间含有脯氨酸。 疫苗组合物含有肽抗原，编码其的核酸或肽抗原的抗体。 生产具有免疫原性的肽抗原的方法包括：确定对应于靶抗原表位的氨基酸序列的步骤; 以及制备包含两个或更多个串联重复氨基酸序列单元的多肽的步骤; 或制备另外含有脯氨酸的氨基酸序列的步骤。
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