公开(公告)号：KR1020130060605A

公开(公告)日：2013-06-10

申请号：KR1020110126747

申请日：2011-11-30

Applicant: 전남대학교산학협력단

Inventor： 최현일 ,  민정준 ,  정재호 ,  유상렬 ,  임정아

IPC: C07K14/255 ,  C12N15/31 ,  C12N15/63 ,  A61P35/00

CPC classification number: C07K14/255 ,  A61K47/42 ,  A61K47/62 ,  C12N15/63

Abstract: PURPOSE: A novel polypeptide and a polynucleotide encoding the same are provided to properly control bacterial lysis and to effectively release a drug transferred by therapeutic bacteria to a lesion. CONSTITUTION: A polypeptide has holin non-dependent cell lysis activities with 70% or more homology with an amino acid sequence of sequence number 3. The polypeptide has 70% or more homology with an amino acid sequence of sequence number 1. A polynucleotide encodes the polypeptide. A vector contains the polynucleotide. A host cell is transformed with the vector. [Reference numerals] (AA) pLT1::ORF1, Control group; (BB) pLT1::ORF1, Induced; (CC,FF,II,LL,OO,RR) Time(hours); (DD) pLT35-1, Control group; (EE) pLT35-1, Induced; (GG) pLT1::ORF2, Control group; (HH) pLT1::ORF2, Induced; (JJ) pLT33, Control group; (KK) pLT33, Induced; (MM) pLT1::ORF3, Control group; (NN) pLT1::ORF3, Induced; (PP) pLT34, Control group; (QQ) pLT34, Induced

Abstract translation: 目的：提供新的多肽和编码该多肽的多核苷酸以适当地控制细菌裂解并有效地将由治疗性细菌转移的药物释放到病变处。 构成：多肽具有与序列号3的氨基酸序列具有70％或更高同源性的完全非依赖性细胞裂解活性。多肽与序列号1的氨基酸序列具有70％或更高的同源性。多核苷酸编码 多肽。 载体含有多核苷酸。 用载体转化宿主细胞。 （标号）（AA）pLT1 :: ORF1，对照组; （BB）pLT1 :: ORF1，诱导; （CC，FF，II，LL，OO，RR）时间（小时）; （DD）pLT35-1，对照组; （EE）pLT35-1，诱导; （GG）pLT1 :: ORF2，对照组; （HH）pLT1 :: ORF2，诱导; （JJ）pLT33，对照组; （KK）pLT33，诱导; （MM）pLT1 :: ORF3，对照组; （NN）pLT1 :: ORF3，诱导; （PP）pLT34，对照组; （QQ）pLT34，诱导

公开(公告)号：KR101346620B1

公开(公告)日：2014-01-06

申请号：KR1020110126747

申请日：2011-11-30

Applicant: 전남대학교산학협력단

Inventor： 최현일 ,  민정준 ,  정재호 ,  유상렬 ,  임정아

IPC: C07K14/255 ,  C12N15/31 ,  C12N15/63 ,  A61P35/00

Abstract: 본 발명은 원하는 위치와 시간에 외래 단백질 또는 핵산 분자를 전달하기 위해, 홀린 비의존성 세균 용해 활성을 갖거나 홀린 의존성 세균 용해 활성을 갖는 신규 폴리펩티드, 그를 암호화하는 폴리뉴클레오티드 및 그의 용도를 제공한다.