公开(公告)号：KR1020130083267A

公开(公告)日：2013-07-22

申请号：KR1020120003950

申请日：2012-01-12

Applicant: 전남대학교산학협력단

Inventor： 조덕 ,  장윤영 ,  김상기 ,  이제중

IPC: C12Q1/66 ,  C12Q1/02 ,  C12N5/0783 ,  C12N15/85

CPC classification number: C12Q1/66 ,  C12N5/0081 ,  C12N5/0646 ,  C12N15/85 ,  G01N33/5005

Abstract: PURPOSE: A method for measuring the activity of natural killer cells (NK cells) is provided to test with the same accuracy as a standard method (^51Cr release method) using a luciferase expression cell line without radioactive isotopes. CONSTITUTION: A method for measuring the activity of NK cells comprises the steps of: mixing a measuring sample containing the NK cells and a target sample containing a luciferase expression cell line; adding luciferin to the mixture of the measuring sample and the target sample; and measuring light generated while luciferin turns into oxyluciferin by the action of luciferase. The luciferase expression cell line is prepared by inserting luciferase genes into one cell line among K562, ES-8, HL-60, Daudi, and ARH77.

Abstract translation: 目的：提供一种测量自然杀伤细胞（NK细胞）活性的方法，使用不含放射性同位素的荧光素酶表达细胞系，以与标准方法（^ 51Cr释放方法）相同的精确度进行测试。 构成：测量NK细胞活性的方法包括以下步骤：将含有NK细胞的测定样品和含有荧光素酶表达细胞系的靶标样品混合; 将荧光素加入到测量样品和目标样品的混合物中; 并且通过荧光素酶的作用测量荧光素变成氧化荧光素时产生的光。 通过将荧光素酶基因插入到K562，ES-8，HL-60，Daudi和ARH77中的一个细胞系中来制备荧光素酶表达细胞系。