公开(公告)号：KR100970998B1

公开(公告)日：2010-07-20

申请号：KR1020080070807

申请日：2008-07-21

Applicant: 전남대학교산학협력단

Inventor： 심재한 ,  최정희

IPC: G01N33/487 ,  G01N33/48 ,  G01N33/04 ,  G01N33/00

Abstract: 본 발명은 생체시료에서 잔류하는 항생제를 검출하는 방법에 대한 것으로 보다 구체적으로는 초임계유체추출법을 이용하여 액상의 생체시료로부터 플루오로퀴놀론계 항생제를 검출하고 검출된 항생제의 농도를 분석하는 방법에 대한 것이다.
액상 생체시료, 오르비플록사신(orbifloxacin), 초임계유체추출법, 고속액체크로마토그래피

公开(公告)号：KR1020100009963A

公开(公告)日：2010-01-29

申请号：KR1020080070807

申请日：2008-07-21

Applicant: 전남대학교산학협력단

Inventor： 심재한 ,  최정희

IPC: G01N33/487 ,  G01N33/48 ,  G01N33/04 ,  G01N33/00

Abstract: PURPOSE: A method for detecting antibiotics remaining in bio sample is provided to simplify process using general extraction container and reduce discharge of contamination pollutant. CONSTITUTION: A method for detecting antibiotics contained in liquid bio sample using SFE(supercritical fluid extraction) comprises: a step of inserting absorption member into SFE extraction container; a step of absorbing liquid bio sample in the absorption member; and a step of extracting fluoroquinolone antibiotics contained in bio sample under process condition. The process condition is 20-30% of extraction aid content, 120-150°C, 250-320kg/cm^2 and extraction time 20-40. The fluoroquinolone antibiotics are orbifloxacin.

Abstract translation: 目的：提供一种检测生物样品中残留的抗生素的方法，以简化使用一般提取容器的过程，减少污染物排放。 构成：使用SFE（超临界流体萃取）检测液体生物样品中所含的抗生素的方法包括：将吸收构件插入SFE提取容器中的步骤; 在吸收构件中吸收液体生物样品的步骤; 在生物样品中提取氟喹诺酮类抗生素的步骤。 工艺条件为萃取助剂含量为20-30％，120-150℃，250-320kg / cm ^ 2，萃取时间为20-40℃。 氟喹诺酮类抗生素是orbifloxacin。

公开(公告)号：KR1020100009940A

公开(公告)日：2010-01-29

申请号：KR1020080070780

申请日：2008-07-21

Applicant: 전남대학교산학협력단

Inventor： 심재한 ,  최정희

IPC: G01N33/48 ,  G01N33/00

Abstract: PURPOSE: A method for detecting pyrethroid agricultural chemical remained in animal bio sample is provided to reduce detection time and cost. CONSTITUTION: A method for detecting pyrethroid agricultural chemical contained in animal bio sample comprises: a step of homogenizing animal bio sample remaining agricultural chemical; a step of adding organic solvent 1 to homogenized animal bio sample and firstly centrifuging; a step of collecting supernatant, adding organic solvent 2, and centrifuging secondly; a step of removing supernatant of the sample and concentrating; and a step of concentrating to obtain extract. The supernatant is organic solvent. The organic solvent 1 is hexane and organic solvent 2 is acetonitrile. The pyrethroid agricultural chemical is cyfluthrin, cyhalothrin, cypermethrin or deltamethrin.

Abstract translation: 目的：提供一种检测动物生物样品中拟除虫菊酯农药残留的方法，以减少检测时间和成本。 构成：检测动物生物样品中拟除虫菊酯农药的检测方法包括：将动物生物样品剩余农药均质化的步骤; 首先将有机溶剂1加入匀浆的动物生物样品中，首先离心; 收集上清液，加入有机溶剂2，再次离心的步骤; 去除样品上清液并浓缩的步骤; 和浓缩步骤以获得提取物。 上清液为有机溶剂。 有机溶剂1是己烷，有机溶剂2是乙腈。 拟除虫菊酯农药是氟氯氰菊酯，氯氟氰菊酯，氯氰菊酯或溴氰菊酯。

公开(公告)号：KR1020090001089A

公开(公告)日：2009-01-08

申请号：KR1020070065195

申请日：2007-06-29

Applicant: 전남대학교산학협력단

Inventor： 심재한 ,  최정희

IPC: C07D215/24 ,  C07D215/48

CPC classification number: Y02P20/544

Abstract: A method for extracting enrofloxacin, danofloxacin and ciprofloxacin of fluoroquinolone-based antibiotics simultaneously from pork is provided to simplify extraction process by using supercritical fluid extraction. A method for extracting enrofloxacin, danofloxacin and ciprofloxacin of fluoroquinolone-based antibiotics simultaneously from pork comprises the step of extracting enrofloxacin, danofloxacin and ciprofloxacin from pork by using the supercritical carbon dioxide containing 10 % or more of an extraction aid at an extraction temperature of 40 deg.C or more and at an extraction pressure of 250 kg/cm^2 or more for 30 min or more by the supercritical fluid extraction.

Abstract translation: 提供从猪肉同时提取氟喹诺酮类抗生素的恩诺沙星，丹诺沙星和环丙沙星的方法，以通过超临界流体萃取简化萃取过程。 从猪肉中同时提取氟喹诺酮类抗生素的恩诺沙星，丹诺沙星和环丙沙星的方法包括采用超临界二氧化碳提取恩硝沙星，丹诺沙星和环丙沙星的方法，提取温度为40℃时，使用含10％以上萃取助剂的超临界二氧化碳 ℃以上，通过超临界流体萃取，萃取压力为250kg / cm 2以上30分钟以上。

公开(公告)号：KR100880012B1

公开(公告)日：2009-01-22

申请号：KR1020070065195

申请日：2007-06-29

Applicant: 전남대학교산학협력단

Inventor： 심재한 ,  최정희

IPC: C07D215/24 ,  C07D215/48

CPC classification number: Y02P20/544

Abstract: 본 발명은 돈육에 포함된 플루오로퀴놀론계 항생제인 엔로플록사신, 다노플록사신 및 시프로플록사신을 초임계유체추출법을 이용하여 동시추출하는 추출 공정 조건을 제공하고, 상기 초임계유체추출법을 이용하여 추출한 플루오로퀴놀론계 항생제들을 분석하는 방법을 제공하는 초임계유체추출법을 이용한 돈육에서 플루오로퀴놀론계 항생제인 엔로플록사신, 다노플록사신 및 시프로플록사신의 동시추출 및 분석 방법에 관한 것이다.
돈육, 항생제, 초임계유체추출, 동시추출