公开(公告)号：KR1020070099899A

公开(公告)日：2007-10-10

申请号：KR1020060031251

申请日：2006-04-06

Applicant: 조선대학교산학협력단

Inventor： 유진철 ,  이효정 ,  김재일 ,  김수민 ,  김성준

IPC: A61K31/35 ,  A61K47/42 ,  A61P31/04

CPC classification number: A61K31/35 ,  A61K47/18 ,  A61K47/6901 ,  A61K2121/00

Abstract: A polyether antibiotics-carrier protein conjugate is provided to be used as an antigen for producing a monoclonal antibody specifically bound to the polyether antibiotics, thereby allowing the obtained monoclonal antibody to be used for rapidly and precisely detecting a very small amount of the polyether antibiotics contained in a mixture such as an animal feed. A method for preparing a polyether antibiotics-carrier protein conjugate comprises a step of: reacting a compound obtained by reacting a polyether antibiotics having a carboxyl group with a compound having a carbodiimide group with a carrier protein having an amine nucleophilic group; or reacting a polyether antibiotics having a carboxyl group with a mixture anhydride in the presence of a solvent and then reacting it with a carrier protein having an amine nucleophilic group, wherein the polyether antibiotics are selected from the group consisting of monensin, salinomycin, laidlomycin, narasin, lasalocid and maduramicin and the carrier protein is selected from the group consisting of bovine serum albumin(BSA, ovalbumin and keyhole limpet hemocyanin( KLH)).

Abstract translation: 提供聚醚抗生素 - 载体蛋白结合物作为用于制备与聚醚抗生素特异性结合的单克隆抗体的抗原，从而使得到的单克隆抗体用于快速且精确地检测非常少量的包含的聚醚抗生素 在混合物如动物饲料中。 制备聚醚抗生素 - 载体蛋白结合物的方法包括以下步骤：使具有羧基的聚醚抗生素与具有碳二亚胺基团的化合物反应得到的化合物与具有胺亲核基团的载体蛋白质反应; 或在溶剂存在下使具有羧基的聚醚类抗生素与混合酸酐反应，然后使其与具有胺亲核基团的载体蛋白反应，其中所述聚醚抗生素选自莫能菌素，盐霉素，舍洛霉素， 纳沙星，拉沙里菌素和马杜米霉素，载体蛋白选自牛血清白蛋白（BSA，卵清蛋白和匙孔血蓝蛋白（KLH））。