公开(公告)号：KR1020100084817A

公开(公告)日：2010-07-28

申请号：KR1020090004165

申请日：2009-01-19

Applicant: 조선대학교산학협력단

Inventor： 유호진 ,  장인엽 ,  이정희 ,  강윤성 ,  김홍범 ,  강미영

IPC: A61K38/16 ,  A61P35/00

CPC classification number: A61K38/1758 ,  C07K14/4703 ,  C07K14/4746 ,  C12N15/63

Abstract: PURPOSE: An anticancer drug containing p53 fragment A is provided to suppress MEK/ERK activation and infiltration of cancer cells. CONSTITUTION: A polypeptide having anti-cancer activity contains 46-340 amino acids of sequence number 2. The polypeptide contains 160-206th amino acid in p53R2 amino acid sequence of sequence number 1. The polypeptide comprises an amino acid sequence selected from sequence numbers 2-4. An expression vector contains a polynucleotide encoding the polypeptide. An anticancer drug contains the polypeptide or the expression vector containing the same as an active ingredient. The cancer is lung cancer, breast cancer, ovarian cancer, kidney cancer, or prostate cancer.

Abstract translation: 目的：提供含有p53片段A的抗癌药物，以抑制MEK / ERK活化和癌细胞浸润。 构成：具有抗癌活性的多肽含有序列号2的46-340个氨基酸。该多肽在序列1的p53R2氨基酸序列中含有160-206位氨基酸。该多肽包含选自序列号2的氨基酸序列 -4。 表达载体含有编码多肽的多核苷酸。 抗癌药含有与活性成分相同的多肽或表达载体。 癌症是肺癌，乳腺癌，卵巢癌，肾癌或前列腺癌。

公开(公告)号：KR101077179B1

公开(公告)日：2011-10-27

申请号：KR1020090004526

申请日：2009-01-20

Applicant: 조선대학교산학협력단

Inventor： 유호진 ,  장인엽 ,  이정희 ,  강윤성 ,  김홍범 ,  강미영

IPC: A61K31/7052 ,  A61P35/00 ,  A61K8/64 ,  A61Q19/08

Abstract: 본발명은 PIG3를이용한 DNA 손상보호방법에관한것으로, 보다상세하게는 PIG3(p53-inducible gene 3)가자외선(UV) 또는방사능유사약물(radiomimetic drug) 등의 DNA 손상인자(DNA damaging agents)에대한세포의민감도를억제하고, DNA 손상에대한회복에관여하며, DNA 손상점검점경로의활성에관여하며, DNA 손상에대한반응에서핵내응집체를형성하고, Chk1, Chk2 및 H2AX의인산화에관여하며, ATM 및 ATR의다운스트림으로기능함으로써, 상기 PIG3가 DNA 손상반응경로의중요한구성성분이며 DNA 손상신호전달에직접적인역할을하는것을알 수있으므로 DNA 손상보호에유용하게이용할수 있다.

公开(公告)号：KR1020100085316A

公开(公告)日：2010-07-29

申请号：KR1020090004527

申请日：2009-01-20

Applicant: 조선대학교산학협력단

Inventor： 유호진 ,  장인엽 ,  김미화 ,  김홍범

IPC: A61K38/16 ,  A61P25/28

CPC classification number: A61K38/1709 ,  C07K14/47

Abstract: PURPOSE: A composition containing Ape1/Ref-1(Apurinic/apyrimidinic endonuclease 1) is provided to induce neurite generation, to protect neural cells, and to prevent and treat neurodegenerative diseases. CONSTITUTION: A polypeptide enhancing the expression of glial cell-derived neurotropic factor receptor alpha 1(GFR alpha 1) comprises 100-150 amino acids contains 1-100th amino acids which are Ape1/Ref-1 oxidation/reduction domain. The Ape1/Ref-1 oxidation/reduction domain has an amino acid sequence of sequence number 2. A polynucleotide encoding the polypeptide has a nucleotide sequence of sequence number 3. A composition for preventing and treating neurodegenerative diseases contains the Ape1/Ref-1 oxidation/reduction domain or expression vector thereof.

Abstract translation: 目的：提供含有Ape1 / Ref-1（Apurinic / apyrimidinic内切核酸酶1）的组合物，以诱导神经突生成，保护神经细胞，并预防和治疗神经变性疾病。 构成：增强神经胶质细胞衍生的神经营养因子受体α1（GFRα1）的表达的多肽包含100-150个氨基酸，其含有第1-100个氨基酸，其是Ape1 / Ref-1氧化/还原结构域。 Ape1 / Ref-1氧化/还原结构域具有序列号2的氨基酸序列。编码多肽的多核苷酸具有序列号3的核苷酸序列。用于预防和治疗神经变性疾病的组合物含有Ape1 / Ref-1氧化 /还原结构域或其表达载体。

公开(公告)号：KR1020100085317A

公开(公告)日：2010-07-29

申请号：KR1020090004528

申请日：2009-01-20

Applicant: 조선대학교산학협력단

Inventor： 유호진 ,  장인엽 ,  이정희 ,  강윤성 ,  김홍범 ,  강미영

IPC: C12Q1/68 ,  G01N33/533

CPC classification number: G01N33/5011 ,  G01N33/533 ,  G01N2500/00

Abstract: PURPOSE: A method for screening an inhibitor of AMPK activation is provided to select a material which suppresses inter-combination of AMPKγ1 and DNA-PKcs and to use as an anticancer drug. CONSTITUTION: A method for screening drug for suppressing cancer proliferation and/or metastasis or protecting nerve comprises: a step of treating a candidate material which suppresses inter-combination of AMPKγ1 and DNA-PKcs (experimental group); a step of measuring activation of inter-combination of AMPKγ1 and DNA-PKcs(positive control group) and inter-combination of the experimental group; and a step of selecting candidate material over the positive control group.

Abstract translation: 目的：提供一种筛选AMPK活化抑制剂的方法，以选择抑制AMPKγ1和DNA-PKcs相互组合的材料，并用作抗癌药物。 构成：用于筛选抑制癌细胞增殖和/或转移或保护神经的药物的方法包括：处理抑制AMPKγ1和DNA-PKcs（实验组）的组合的候选物质的步骤; 测量AMPKγ1和DNA-PKcs（阳性对照组）和实验组相互组合的组合活化的步骤; 以及在阳性对照组中选择候选物质的步骤。

公开(公告)号：KR1020100085315A

公开(公告)日：2010-07-29

申请号：KR1020090004526

申请日：2009-01-20

Applicant: 조선대학교산학협력단

Inventor： 유호진 ,  장인엽 ,  이정희 ,  강윤성 ,  김홍범 ,  강미영

IPC: A61K31/7052 ,  A61P35/00 ,  A61K8/64 ,  A61Q19/08

CPC classification number: A61K31/7105 ,  A61K8/606

Abstract: PURPOSE: A composition and method for protecting DNA damage using PIG3 are provided to suppress cytotoxicity and to relate to normal recognition in DNA damage. CONSTITUTION: An anti-cancer aid contains an inhibitor for expression or activation of PIG3(p53-inducible gene 3). The PIG3 has an amino acid sequence of sequence number 1. The inhibitor for expression of PIG3 is an antisense nucleotide which complementarily binds to mRNA of PIG3 gene, small interfering RNA(siRNA) or small hairpin RNA(shRNA). The inhibitor for expression or activation of PIG3 treats and prevents cancer by administering to cancer cells. A cosmetic composition for anti-aging contains PIG3 gene construct as an active ingredient. The PIG3 gene has a nucleotide sequence of sequence number 2.

Abstract translation: 目的：提供使用PIG3保护DNA损伤的组合物和方法，以抑制细胞毒性并涉及DNA损伤的正常识别。 构成：抗癌助剂含有用于表达或激活PIG3（p53-诱导型基因3）的抑制剂。 PIG3具有序列号1的氨基酸序列.PIG3表达抑制剂是与PIG3基因，小干扰RNA（siRNA）或小发夹RNA（shRNA）的mRNA互补结合的反义核苷酸。 用于表达或激活PIG3的抑制剂通过给予癌细胞治疗并预防癌症。 用于抗衰老的化妆品组合物包含PIG3基因构建体作为活性成分。 PIG3基因具有序列号2的核苷酸序列。