公开(公告)号：KR100852255B1

公开(公告)日：2008-08-14

申请号：KR1020070063885

申请日：2007-06-27

Applicant: 조선대학교산학협력단

Inventor： 김시욱 ,  김희곤 ,  김혁한 ,  최영봉

IPC: G01N31/10 ,  G01N31/00

Abstract: A method for detecting trimethylamine, a biosensor used in the detection method, and a method for preparing the biosensor are provided to detect harmful trimethylamine quantitatively on the spot. A method for detecting trimethylamine comprises the step of detecting the catalyst current generated by the enzyme/substrate oxidation-reduction reaction between trimethylamine dehydrogenase(TMADH) and a sample at a working electrode where a gold nanoparticle is vapor deposited and an osmium complex PAAMP-Os represented by the formula 1 as an electron transfer medium is fixed, with an electrochemical signal. Preferably the working electrode is a screen printed carbon paste electrode(SPE).

Abstract translation: 检测三甲胺的方法，检测方法中使用的生物传感器以及制备生物传感器的方法，用于定量检测有害的三甲胺。 检测三甲胺的方法包括检测在三甲胺脱氢酶（TMADH）和金纳米颗粒气相沉积的工作电极处的样品之间的酶/底物氧化 - 还原反应产生的催化剂电流的步骤和锇络合物PAAMP-Os 用电化学信号固定由式1表示的电子转移介质。 优选地，工作电极是丝网印刷碳糊电极（SPE）。