公开(公告)号：KR101263452B1

公开(公告)日：2013-05-10

申请号：KR1020110053429

申请日：2011-06-02

Applicant: 조선대학교산학협력단

Inventor： 박윤경 ,  박성철 ,  박진순

IPC: C07K14/435 ,  A61K38/17 ,  A61K8/64 ,  A61P31/04

CPC classification number: Y02A50/473

Abstract: 본발명은키조개(Korean pen shell,)에서추출한신규한열 안정성펩타이드에관한것으로, 보다구체적으로본 발명의키조개에서추출한신규한열 안정성펩타이드는그람음성균에대한강력한항균활성을나타내고독성이없으므로본 발명의펩타이드를유효성분으로함유하는항균제, 화장품보존제, 의약품보존제및 생물농약으로유용하게사용될수 있다.

公开(公告)号：KR1020120134501A

公开(公告)日：2012-12-12

申请号：KR1020110053429

申请日：2011-06-02

Applicant: 조선대학교산학협력단

Inventor： 박윤경 ,  박성철 ,  박진순

IPC: C07K14/435 ,  A61K38/17 ,  A61K8/64 ,  A61P31/04

CPC classification number: Y02A50/473

Abstract: PURPOSE: A novel thermal stability peptide isolated from Atrina pectinata is provided to ensure strong antibacterial activity against gram-negative bacteria without toxicity and to be used in an antibacterial agent, a cosmetic preservative, and a biological acgrochemical. CONSTITUTION: An antibacterial peptide with thermal stability is isolated from Atrina pectinata. A method of preparing the antibacterial peptides comprises: a step of freeze-drying and pulverizing Atrina pectinata; a step of extracting the Atrina pectinata powder with an organic solvent; a step of centrifuging the extract and collecting a supernatant fraction; a step of heating the supernatant fraction and collecting thermally stable fractions; and a step of performing chromatography and dialysis of the fraction. The organic solvent is 50%(v/v) methanol solution. An antibacterial pharmaceutical composition contains the antibacterial peptides as an active ingredient.

Abstract translation: 目的：提供从Atrina pectinata分离的新型热稳定肽，以确保对革兰氏阴性细菌无毒性的强抗菌活性，并用于抗菌剂，化妆品防腐剂和生物化学品。 构成：从Atrina pectinata中分离出具有热稳定性的抗菌肽。 制备抗菌肽的方法包括：冷冻干燥和粉碎Atrina pectinata的步骤; 用有机溶剂提取阿奇利菌素粉末的步骤; 离心提取物并收集上清液部分的步骤; 加热上清液部分并收集热稳定级分的步骤; 以及进行该级分的层析和透析的步骤。 有机溶剂为50％（v / v）甲醇溶液。 抗菌药物组合物含有抗菌肽作为活性成分。