公开(公告)号：KR101884394B1

公开(公告)日：2018-08-02

申请号：KR1020170013933

申请日：2017-01-31

Applicant: 조선대학교산학협력단

Inventor： 이정섭 ,  박정은 ,  임도성 ,  조영희 ,  안철 ,  임가은

IPC: C12N9/52 ,  A23L29/00 ,  A23L33/18 ,  A61K38/48

Abstract: 본발명은비브리오프루니씨() 유래의재조합혈전분해효소, 상기재조합혈전분해효소를유효성분으로함유하는혈전분해용조성물, 상기조성물을혈전을포함하고있는개체에처리하여혈전을분해하는방법, 상기재조합혈전분해효소를코딩하는유전자를포함하는재조합벡터로형질전환된재조합미생물을이용하여재조합혈전분해효소를생산하는방법, 상기방법에의해생산된재조합혈전분해효소, 상기재조합혈전분해효소를유효성분으로포함하는혈전증의예방또는개선용건강기능식품조성물및 약학조성물에관한것으로, 본발명의재조합혈전분해효소는혈전증또는이와유사한질환의치료및 예방에효과적으로활용될수 있을것이다.

公开(公告)号：KR102164490B1

公开(公告)日：2020-10-12

申请号：KR1020180102177

申请日：2018-08-29

Applicant: 조선대학교산학협력단

Inventor： 이정섭 ,  박정은 ,  이건호 ,  김병채 ,  임도성 ,  조영희

IPC: G01N33/68

公开(公告)号：KR1020120140388A

公开(公告)日：2012-12-31

申请号：KR1020110060094

申请日：2011-06-21

Applicant: 조선대학교산학협력단

Inventor： 이정섭 ,  박정은 ,  박종우

IPC: C12Q1/37 ,  C12N9/52 ,  C07K7/00 ,  C12N1/20

CPC classification number: C07K7/64 ,  C12Q1/37 ,  C12Y304/24 ,  G01N33/52

Abstract: PURPOSE: A fluorescent substrate for protease with broad substrate specificity and a diagnostic kit containing the same are provided to accurately measure protease activity and to identify bacteria presence. CONSTITUTION: A flurescent substrate is specific to secretory protease and has chemical formula 1(MCA-Xn-A-Xm-DNP). The bacteria are vibrio vulnificus including Vibrio vulnificus, Vibrio mimicus, Vibrio fluvialis, Vibrio furnissii, Vibrio hollisae, Vibrio alginolyticus, Vibrio cincinnatiensis, or Vibrio metschnikovii. The Vibrio vulnificus is Vibrio vulnificus ATCC29307. The protease is metalloprotease. A diagnostic kit contains the fluorescent substrate. The kit is used for measuring the presence and content of the protease by MCP luminescence.

Abstract translation: 目的：提供具有广泛底物特异性的蛋白酶的荧光底物和含有其的诊断试剂盒，以准确测定蛋白酶活性并鉴定细菌存在。 构成：具有化学式1（MCA-Xn-A-Xm-DNP）的分泌蛋白酶特异性的底物底物。 细菌是创伤弧菌，包括创伤弧菌，弧菌弧菌，弧菌弧菌，弧菌弧菌，解酒弧菌，辛弧菌弧菌或弧菌弧菌。 创伤弧菌是创伤弧菌ATCC29307。 蛋白酶是金属蛋白酶。 诊断套件包含荧光底物。 该试剂盒用于通过MCP发光测量蛋白酶的存在和含量。

公开(公告)号：KR1020120095231A

公开(公告)日：2012-08-28

申请号：KR1020110014774

申请日：2011-02-18

Applicant: 조선대학교산학협력단

Inventor： 이정섭 ,  박정은 ,  박종우

IPC: C07K19/00 ,  C12N9/52 ,  C12N15/62 ,  C07K16/12

Abstract: PURPOSE: A septicemia vibrio vulnificus originated recombinant protein is provided to rapidly and accurately determine the existence of Septicemia vibrio vulnificus and an antibody for the recombinant protein is provided to diagnose vibrio infections. CONSTITUTION: A septicemia vibrio vulnificus originated recombinant protein is a recombinant fusion protein in which the amino-terminal part of metalloprotease is derivated from the Septicemia Vibrio Vulnificus and maltose are combined. The amino-terminal part of the metalloprotease does not include zymophore. The amino-terminal part of the metalloprotease is the amino terminal 139 amino acid of the metalloprotease. The amino-terminal part of the metalloprotease has the amino acid sequence described in the sequence number 1(SEQ ID NO:1) (N-ter139 peptide). The recombinant vector includes the recombination gene which codes the recombinant fusion protein. The recombinant vector is plasmid vector, cosmid vector, bacteriophage vector or virus vector. The recombinant vector is plasmid pN-ter139. The transformed recombinant vector is Escherichia coli, bacillus, streptomycin, pseudomonas, proteus mirabilis, star pillow yeast, bacillus, fungus, yeast, insect cell, plant cell or mammal.

Abstract translation: 目的：提供败血症创伤性重组蛋白，以快速，准确地确定创伤弧菌的存在，并提供重组蛋白的抗体来诊断弧菌感染。 构成：创伤性败血症创伤重组蛋白是重组融合蛋白，其中金属蛋白酶的氨基末端部分衍生自败血性弧菌弧菌和麦芽糖。 金属蛋白酶的氨基末端部分不包括酶原。 金属蛋白酶的氨基末端是金属蛋白酶的氨基末端139个氨基酸。 金属蛋白酶的氨基末端部分具有序列号1（SEQ ID NO：1）（N-ter139肽）中描述的氨基酸序列。 重组载体包括编码重组融合蛋白的重组基因。 重组载体为质粒载体，粘粒载体，噬菌体载体或病毒载体。 重组载体是质粒pN-ter139。 转化的重组载体是大肠杆菌，杆菌，链霉素，假单胞菌，奇异变形杆菌，星型枕头酵母，杆菌，真菌，酵母，昆虫细胞，植物细胞或哺乳动物。