公开(公告)号：KR100807196B1

公开(公告)日：2008-02-28

申请号：KR1020060128398

申请日：2006-12-15

Applicant: 조선대학교산학협력단

Inventor： 유진철 ,  김태성 ,  최진호 ,  김승현 ,  이효정 ,  김성준 ,  하정완

IPC: A61K35/618 ,  A61K9/00

CPC classification number: A61K35/618 ,  A61K9/0053

Abstract: An extract of Scapharca broughtonii is provided to be able to inhibit the induction of promyelocytic leukemia and auto-immune diseases, thereby being widely utilized as an anti-cancer agent or for treating various immune-diseases. A pharmaceutical composition for treating promyelocytic leukemia comprises an alcohol extract of Scapharca broughtonii and a pharmaceutically acceptable carrier. A method for preparing an alcohol fraction of the Scapharca broughtonii comprises the steps of: (a) after removing muscle from the Scapharca broughtonii and washing it with ice-cooled salt, freeze-drying it; (b) after finely cutting the frozen muscle and dipping it in a mixture solvent of ethanol and water in a volumetric ratio of 60:40 at room temperature of 3-10 days, evaporating an ethanol-aqueous solution obtained by removing remaining solid phase materials therefrom through centrifugation under reduced pressure to eliminate the ethanol; (c) adding activated carbon to the aqueous solution and agitating it; (d) separating the activated carbon from the aqueous solution through filtration and washing it with distilled water; (e) respectively eluting effective ingredients from the activated carbon using an 80% aqueous solution of acetone and 100% ethanol; (f) putting two eluents together and drying it under reduced pressure; (g) subjecting the dried material to a stepwise elution using 100% ethyl acetate, 100% ethanol and 100% methanol to fractionate; and (h) respectively concentrating the obtained ethanol fraction and methanol fraction and respectively purifying them through a silica-gel chromatography.

Abstract translation: 提供石斛提取物以能够抑制早幼粒细胞白血病和自身免疫疾病的诱导，从而被广泛用作抗癌剂或用于治疗各种免疫疾病。 一种用于治疗早幼粒细胞白血病的药物组合物，其包含石菖蒲的酒精提取物和药学上可接受的载体。 用于制备石菖蒲酒精馏分的方法包括以下步骤：（a）从玻璃after after中除去肌肉后，用冰冷却的盐洗涤，冷冻干燥; （b）精细切割冷冻肌肉后，在3-10天的室温下将体积比为60:40的乙醇和水的混合溶剂浸渍，蒸发除去剩余的固相物质得到的乙醇 - 水溶液 通过在减压下离心以除去乙醇; （c）向水溶液中加入活性炭并搅拌; （d）通过过滤从水溶液中分离活性炭并用蒸馏水洗涤; （e）使用80％丙酮和100％乙醇水溶液分别从活性炭洗脱有效成分; （f）将两种洗脱液放在一起，减压干燥; （g）使用100％乙酸乙酯，100％乙醇和100％甲醇对干燥的物质进行逐步洗脱以分馏; 和（h）分别浓缩得到的乙醇级分和甲醇级分，并通过硅胶色谱法分别纯化。