Abstract:
PURPOSE: A usage for health promoting food, a whitening cosmetic product, and a medicine using a seaweed methanol extract are provided to offer various biological activities including an antioxidant effect, a whitening effect, and an anti-inflammatory effect. CONSTITUTION: Porphyra yezoensis, Undaria pinnatifida, Hizikia fusiformis, Capsosiphon fulvescens, Enteromorpha Linza are collected. The seaweeds are washed and dried, then soaked in 500ml of 70% methanol for 100g of seaweeds before extracting for a week. The extract is filtered by using filtering paper. The filtered material is freeze-dried after the concentration by using an evaporator. [Reference numerals] (AA) Hizikia fusiformis; (BB) Porphyra yezoensis; (CC) Undaria pinnatifida; (DD) Enteromorpha Linza; (EE) Capsosiphon fulvescens
Abstract:
PURPOSE: A method for producing hPGF-BB(human platelet-derived growth factors) from transformed Pleurotus eryngii is provided to establish mass production system of transformed mycelium. CONSTITUTION: A method for producing hPGF-BB transformed with a vector from Pleurotus eryngii comprises: a step of constructing the vector containing a gene of hPGF-BB; a step of transforming a secretion vector into Pleurotus eryngii; a step of isolating each genome DNA and confirming transformant of the recombinant proteins by PCR or detecting gene expression of the transformant by southern blot or northern blot; and a step of isolating recombinant proteins from the transformant.
Abstract:
본 발명은 분비 백터를 도입시켜 형질전환된 새송이버섯으로부터 재조합 단백질을 생산하는 방법 및 이 방법에 의해 제조된 재조합 단백질에 관한 것이다. 본 발명에 따르면, 형질전환 균사체의 대량생산 체계 확립과 생산된 균사체의 단백질 정제 및 생물학적 활성연구가 병행되어지면 재조합 단백질 생산을 위한 새로운 미생물 생산시스템으로의 활용이 가능할 것으로 사료되어진다.