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公开(公告)号:KR1019950006950B1
公开(公告)日:1995-06-26
申请号:KR1019910022989
申请日:1991-12-14
Applicant: 주식회사대성미생물연구소
IPC: C12P1/00
Abstract: A selenized yeast is prepd. by i) innoculating 0.1 ppm or more selenium-contg. YM medium with saccharomyces uvarium, ii) fermenting it at 30 deg. C, 1 vvm and 500 rpm for 8 hrs., iii) adding selenium salt and 5 g/l glucose into the fermented broth, iv) re-fermenting the obtd. mixed broth at 27-28 deg. C, 1 vvm and 500 rpm for 16 hrs., and filtering, and v) washing the obtd. filtrate and drying to obtain the yeast cell containing selenium.
Abstract translation: 硒化酵母是制备的。 通过i)接种0.1ppm或更多硒硒。 具有酵母酵母的YM培养基,ii)在30℃下发酵。 C,1vvm和500rpm 8小时,iii)向发酵液中加入硒盐和5g / l葡萄糖,iv)重新发酵。 混合肉汤在27-28度 C,1vvm和500rpm下搅拌16小时,过滤,v)洗涤。 滤液并干燥得到含有硒的酵母细胞。
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公开(公告)号:KR1019930013122A
公开(公告)日:1993-07-21
申请号:KR1019910022989
申请日:1991-12-14
Applicant: 주식회사대성미생물연구소
IPC: C12P1/00
Abstract: 내용 없음
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公开(公告)号:KR100092586B1
公开(公告)日:1995-12-06
申请号:KR1019910022989
申请日:1991-12-14
Applicant: 주식회사대성미생물연구소
IPC: C12P1/00
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公开(公告)号:KR1019920009497B1
公开(公告)日:1992-10-17
申请号:KR1019910001232
申请日:1991-01-25
Applicant: 한국과학기술연구원 , 주식회사대성미생물연구소
IPC: C12N7/04
Abstract: A method for isolating Aujeszky's virus antigen protein comprises (a) adding phosphate buffer and sodium dodecyl sulfate into the culture soln. which obtd. by inoculating Aujeszky's virus AJHK 3 (KCTC VR 15001) into pig kidney cell, and (b) passing the soln. through cellulose sulfate gel to obtain antigen protein. Also claimed is a diagnostic kit for Aujeszky's disease which comprises polyethylene glycol, casein and Aujeszky's virus AJHK 3 (KCTC VR 15001) antigen protein.
Abstract translation: 用于分离Aujeszky病毒抗原蛋白的方法包括(a)向培养溶液中加入磷酸盐缓冲液和十二烷基硫酸钠。 哪个可以 通过将Aujeszky病毒AJHK 3(KCTC VR 15001)接种到猪肾细胞中,和(b)通过溶胶。 通过硫酸纤维素凝胶得到抗原蛋白。 还声称是Aujeszky病的诊断试剂盒,其包括聚乙二醇,酪蛋白和Aujeszky病毒AJHK 3(KCTC VR 15001)抗原蛋白。
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