파이타제 발현벡터 PJPK 및 이에 의해 형질전환된바실러스 서브틸리스 PJPK.
    5.
    发明公开
    파이타제 발현벡터 PJPK 및 이에 의해 형질전환된바실러스 서브틸리스 PJPK. 失效
    PHYTASE表达载体,PJPK和BACILLUS SUBTILIS PJPK转换

    公开(公告)号:KR1020000041160A

    公开(公告)日:2000-07-15

    申请号:KR1019980056952

    申请日:1998-12-21

    CPC classification number: C12N15/63 A23K10/16 C12N9/14 C12R1/125

    Abstract: PURPOSE: Phytase expression vector, pJPK has strong BJ27 promoter. Bacillus subtilis pJPK (KCTC 0526BP) transformed with pJPK expresses phytase highly, secrets expressed protein and is nontoxic to animal because of lacking endotoxin such as lipopolysaccharides. CONSTITUTION: Bacillus expression vector pJH27, having strong promoter BJ27, is ligated with 2.2 kb phytase gene originated from Bacillus amyloliquefaciens DS-11. The 2.2kb DNA includes SD sequence of phytase gene complementary to Bacillus subtilis 16S rRNA sequence (UCCUCC) for the overexpression of phytase. Resulting recombinant plasmid, pJPK, is introduced into E. coli JM83. Purified pJPK is transformed into Bacillus subtilis DB104 by protoplast transformation. Modified LB medium is consisted of 1% of potassium phosphate, 0.045% of magnesium sulfate, 4% of glucose, 1% of tryptone, 0.5% of yeast extract, 0.5% of NaCl and trace elements. 500-1000 unit of phytase is added to 1kg of animal feed. Animal experiment with porcine reveals that phytase produced from bacillus effectively degrades phytic acid in the animal feed.

    Abstract translation: 目的:植酸酶表达载体pJPK具有较强的BJ27启动子。 用pJPK转化的枯草芽孢杆菌pJPK(KCTC 0526BP)高度表达植酸酶,秘密表达蛋白质,由于缺乏内毒素,如脂多糖,对动物无毒。 构成:具有强启动子BJ27的芽孢杆菌表达载体pJH27与源自解淀粉芽孢杆菌DS-11的2.2kb植酸酶基因连接。 2.2kb DNA包括与枯草芽孢杆菌16S rRNA序列(UCCUCC)互补的植酸酶基因的SD序列,用于过表达植酸酶。 将得到的重组质粒pJPK导入大肠杆菌JM83中。 纯化的pJPK通过原生质体转化转化到枯草芽孢杆菌DB104中。 改性LB培养基由1%磷酸钾,0.045%硫酸镁,4%葡萄糖,1%胰蛋白胨,0.5%酵母提取物,0.5%NaCl和微量元素组成。 将500-1000单位的植酸酶加入1kg动物饲料中。 猪的动物实验显示,由芽孢杆菌产生的植酸酶有效降解动物饲料中的植酸。

    동물용 복합항생제 조성물
    8.
    发明授权
    동물용 복합항생제 조성물 失效
    复合抗生素组合物用于动物细菌感染

    公开(公告)号:KR100295742B1

    公开(公告)日:2001-07-03

    申请号:KR1019990013597

    申请日:1999-04-16

    Abstract: 본발명은신규동물용복합항생제조성물에관한것으로서, 더욱상세하게는 1:4∼4:1 중량비로혼합된노르플록사신 (norfloxain)과타이로신 (tylosin)의약제학적유효량을포함하는동물용복합항생제조성물에관한것으로서, 가축및 어류의세균성질병치료와예방에유용할뿐만아니라, 고가의신규항균물질을대체할수 있어궁극적으로는축산농가의생산원가를절감시키고약품의축체내잔류도효율적으로최소화시킬수 있는효과가있는동물용복합항생제조성물에관한것이다.

    동물용 약제조성물
    9.
    发明授权
    동물용 약제조성물 失效
    动物药用组合物

    公开(公告)号:KR100131515B1

    公开(公告)日:1998-04-17

    申请号:KR1019940010312

    申请日:1994-05-11

    Abstract: The interaction between Ocytetracycline(OTC) and Tiamulin(TML) is measured by testing antibiotic effect of compound of OTC and TML at minimum inhibition concentration(MIC) to multi-resistant microorganism - gram positive and negative bacteria separated from chicken, pig and bull infected by several bacterial disease. Herein the medicines is produced by the mixing ratio between OTC and TML is 6:1-18:1 to each weight, inhibites the emergence of antibiotics resisting cluster and decreases the toxicity with decreasing amount when combination is inserted.

    Abstract translation: 通过测试OTC和TML的化合物对从鸡,猪和牛感染分离的多重耐药微生物 - 革兰氏阳性和阴性细菌的抑制浓度(MIC)的最小抑制浓度(MIC)的抗生素效应,测量了四环素(OTC)和钛霉素(TML) 几种细菌性疾病。 本发明药物通过OTC与TML的混合比例为6:1-18:1,每次加入时可抑制抗生素抗生素的出现,并在插入组合时减少毒性。

    동물용 약제조성물
    10.
    发明公开
    동물용 약제조성물 失效
    动物药物组成

    公开(公告)号:KR1019950031083A

    公开(公告)日:1995-12-18

    申请号:KR1019940010312

    申请日:1994-05-11

    Abstract: 본 발명은 동물용 약제조성물에 관한 것으로서, 더욱 상세하게는 옥시테트라사이클린과 티아물린이 6 : 1∼18 : 1의 중량비로써 동물, 특히 돼지의 세균성 설사 또는 기관지 폐렴의 치료와 예방에 유용한 동물용 약제조성물에 관한 것이다.

Patent Agency Ranking