公开(公告)号：KR1019950007860A

公开(公告)日：1995-04-15

申请号：KR1019930019192

申请日：1993-09-21

Applicant: 주식회사한국신약

Inventor： 정경수 ,  고경수 ,  한만우

IPC: C12P1/02

Abstract: 본 발명은 펠리누스 린테우스(Phellinus linteus)균사체를 포도당 2%∼10%, 콘스팁리커 0.2%∼5%, 폐효모추출액 0.1%∼1.0%를 필수성분으로 하는 액체배지에서 배양하는 단계와, 배양된 균사체를 열수처리하는 단계를 포함하는 항암물질의 제조방법에 관한 것이다.

公开(公告)号：KR1019970009150B1

公开(公告)日：1997-06-07

申请号：KR1019930019192

申请日：1993-09-21

Applicant: 주식회사한국신약

Inventor： 정경수 ,  고경수 ,  한만우

IPC: C12P1/02

Abstract: The mycelia of Phellinus linteus (KCTC0082BP) is cultured in liquid medium containing glucose 2-10%, corn steep liquor 0.2-5% and waste yeast extracts 0.1-1.0% as essential components at 24-30 C for 2-4 days. Biomass which is precipitated by centrifuging the culture broth is treated with hot water. Then antitumoral immunity substance is extracted in ethanol precipitation and dialysis for 6-12 hrs therefrom.

Abstract translation: 在24-30℃下，将含有葡萄糖2-10％，玉米浆0.2-5％，废酵母提取物0.1-1.0％作为必需组分的液体培养基培养2-4天。 通过离心培养液沉淀的生物质用热水处理。 然后在乙醇沉淀和透析中提取抗肿瘤免疫物质6-12小时。

公开(公告)号：KR100124853B1

公开(公告)日：1997-09-30

申请号：KR1019930019192

申请日：1993-09-21

Applicant: 주식회사한국신약

Inventor： 정경수 ,  고경수 ,  한만우

IPC: C12P1/02

公开(公告)号：KR1019960016895A

公开(公告)日：1996-06-17

申请号：KR1019940030533

申请日：1994-11-19

Applicant: 주식회사한국신약

Inventor： 정경수

IPC: A61K36/07

公开(公告)号：KR100303886B1

公开(公告)日：2001-11-22

申请号：KR1019940030533

申请日：1994-11-19

Applicant: 주식회사한국신약

Inventor： 정경수

IPC: A61K36/07

Abstract: PURPOSE: A method for extracting an anticancer component from Phellinus linteus is provided, thereby mass-producing the anticancer component having an improved anticancer activity from Phellinus linteus. CONSTITUTION: The method for extracting an anticancer component from Phellinus linteus comprises the steps of: cultivating Phellinus linteus in a medium containing sawdust as a main ingredient; adding water, alkali solution and acid solution into the cultivated Phellinus linteus and pulverizing it with a homogenizer; storing it for 2 to 18 hours and heating it at 80 to 100 deg.C at atmospheric pressure or at 110 to 130 deg.C with pressurizing; filtrating the extract under reduced pressure and concentrating it under vacuum; adding 95% ethanol into the concentrate; centrifuging it at 1000 to 8000 rpm and dissolving the precipitate in water; dialyzing the solution with 8000 to 12000 dalton of dialysis membrane or ultrafiltering it; and freeze-drying the product.