公开(公告)号：KR1020130115602A

公开(公告)日：2013-10-22

申请号：KR1020120038051

申请日：2012-04-12

Applicant: 충남대학교산학협력단

Inventor： 이창수 ,  박애리

IPC: C12Q1/02 ,  C12M1/38 ,  C12M1/34 ,  G01N33/48

CPC classification number: B01L3/5027 ,  B01L2300/185 ,  C12M23/16 ,  C12Q1/02

Abstract: PURPOSE: A microfluidic device with a temperature gradient is provided to generate a temperature gradient in a microchannel of a detection channel and to simply measure the effect of temperature on a reaction and an analysis in the microchannel with reproducibility. CONSTITUTION: A microfluidic device with a temperature gradient comprises: a detection channel with an inlet, an outlet, and a microchannel through which a fluid moves from the inlet to the outlet; and channels for controlling temperature, which are mounted at both sides of the microchannel to run parallel with the microchannel. Heating media with different temperatures are circulated in the channels for controlling temperature. The channels for controlling temperature are separated from the detection channel at a predetermined interval. A method for measuring growth properties of a strain using the microfluidic device comprises the steps of: circulating the heating media in the channels for controlling temperature and generating a temperature gradient in the microchannel of the detection channel; injecting a strain culture medium through the inlet and culturing the strain in the microchannel; and measuring growth properties of the cultured strain.

Abstract translation: 目的：提供具有温度梯度的微流体装置，以在检测通道的微通道中产生温度梯度，并简单地测量温度对反应的影响和微通道中的再现性分析。 构成：具有温度梯度的微流体装置包括：具有入口，出口和微通道的检测通道，流体从入口移动到出口; 以及用于控制温度的通道，其安装在微通道的两侧以与微通道平行地延伸。 具有不同温度的加热介质在用于控制温度的通道中循环。 用于控制温度的通道以预定间隔与检测通道分离。 使用微流体装置测量菌株的生长性质的方法包括以下步骤：在用于控制温度的通道中循环加热介质并在检测通道的微通道中产生温度梯度; 通过入口注入菌株培养基并在微通道中培养菌株; 并测量培养菌株的生长特性。