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公开(公告)号：KR101260184B1
公开(公告)日：2013-05-06
申请号：KR1020110085243
申请日：2011-08-25
Applicant: 충남대학교산학협력단
Abstract: 본발명은히스티딘또는이미다졸을포함하는생체물질을특이적으로검출할수 있는바이오센서, 이의제조방법, 이를활용한생체물질의검출방법및 약물의전달을확인하는방법에관한것이다. 구체적으로본 발명의바이오센서는폴리다이아세틸렌(PDA)을필수적으로포함하는리포좀을포함한다. 더욱구체적으로상기리포좀은폴리다이아세틸렌또는그 유도체를기본골격으로하고, Ni-NTA-DOGS 지질, 및메톡시폴리에틸렌글라이콜(MPEG)이접합된폴리다이아세틸렌또는그 유도체를포함하는생체물질검출용리포좀이며, 상기생체물질은히스티딘표지또는이미다졸을포함하는단백질일수 있다. 본발명의폴리다이아세틸렌(PDA) 리포좀에도입된 Ni-NTA DOGS 지질이 His-tag GFP나이미다졸을검출함으로써나타내는색 변화와형광을이용하여센서로써이용될수 있음을확인할수 있었다. 또한, 본발명의 Ni-NTA / MPEG-PCDA / PDA 리포좀은제조방법이간단하고, 따로형광물질을부착시키지않아도되기때문에값이보다저렴하고여러분야에응용이가능하다. 특히단백질운반의수단으로써본 발명의리포좀을이용할경우, 리포좀에결합된단백질이생체내 표적부위에안전하게도입되었는지여부를리포좀자체의형광을통해확인해볼 수있으므로약물전달시스템에도응용이가능하다.
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公开(公告)号：KR1020130022266A
公开(公告)日：2013-03-06
申请号：KR1020110085243
申请日：2011-08-25
Applicant: 충남대학교산학협력단
Abstract: PURPOSE: A polydiacetylene liposome biosensor and a method for manufacturing the same are provided to use the polydiacetylene liposome biosensor in a drug delivery system. CONSTITUTION: A polydiacetylene liposome biosensor contains polydiacetylene(PDA) which is conjugated with Ni-NTA-DOGS lipid, and methoxy polyethyleneglycol(MPEG) or a derivative thereof. The PDA derivative is PCDA(10,12-pentacosadiynoic acid). A method for preparing a liposome containing MPEG-PDA or a derivative thereof for detecting a biomaterial comprises: a step of dissolving PDA or the derivative thereof, Ni-NTA-DOGS lipid, and MPEG-PDA or the derivative thereof in an organic solvent; a step of volatilizing the organic solvent; a step of adding distilled water, and heating the mixture in a water bath; and a step of performing sonication and filtering.
Abstract translation: 目的：提供聚二乙炔脂质体生物传感器及其制造方法，以在药物递送系统中使用聚二乙炔脂质体生物传感器。 构成：聚二乙炔脂质体生物传感器含有与Ni-NTA-DOGS脂质和甲氧基聚乙二醇（MPEG）或其衍生物结合的聚二乙炔（PDA）。 PDA衍生物是PCDA（10,12-二十五碳二烯酸）。 一种含有MPEG-PDA或其衍生物的脂质体的制备方法，包括：将PDA或其衍生物，Ni-NTA-DOGS脂质和MPEG-PDA或其衍生物溶于有机溶剂中的步骤; 挥发有机溶剂的步骤; 加入蒸馏水的步骤，并在水浴中加热混合物; 并执行超声处理和过滤的步骤。
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