公开(公告)号：KR101334159B1

公开(公告)日：2013-11-27

申请号：KR1020130049461

申请日：2013-05-02

Applicant: 충남대학교산학협력단

Inventor： 김상겸 ,  최종민

IPC: G01N33/53 ,  G01N33/48 ,  G01N33/15

Abstract: The present invention relates to a method for screening hepatotoxicity of a drug metabolite using human liver microsome (HLM), comprising steps of: 1) culturing hepatocarcinoma cells and treating hepatocarcinoma cells with a test material and HLM; 2) exchanging a medium and culturing the hepatocarcinoma cells; and confirming cytotoxicity in the hepatocarcinoma cells. [Reference numerals] (AA) Cell survival rate (the rate compared to a control group)

Abstract translation: 本发明涉及使用人肝微粒体（HLM）筛选药物代谢物的肝毒性的方法，包括以下步骤：1）用测试材料和HLM培养肝癌细胞和治疗肝癌细胞; 2）交换培养基并培养肝癌细胞; 并确认肝细胞中的细胞毒性。 （附图标记）（AA）细胞存活率（与对照组相比的速率）