公开(公告)号：KR1020090067824A

公开(公告)日：2009-06-25

申请号：KR1020070135614

申请日：2007-12-21

Applicant: 포항공과대학교 산학협력단

Inventor： 김경태 ,  박도영 ,  강태홍

IPC: G01N33/574 ,  G01N33/68 ,  G01N33/53 ,  G01N33/48

Abstract: A method for detecting a material which regulates activation of vaccinia-related kinase 1(VRK1) is provided to improve the efficiency of detection of the material. A method for detecting a material which regulates VRK1 activity comprises: a step of treating a histone H3 as a candidate material, VRK1, and substrate of VRK1; a step of measuring the phosphorylation of histone H3; and a step of comparing the phosphorylation degree between the histone H3 treating candidate material and untreated histone H3 The step of measuring phosphorylation comprises: a step of attaching reaction protein with nitrocellulose membrane; and a step of measuring phosphorylation of histone H3 using histone H3-phosphorylated Thr3 and Ser10 or immune antibody which specifically recognizes the histone H3-phosphorylated Thr3 and Ser10.

Abstract translation: 提供了一种检测调节痘苗相关激酶1（VRK1）活化的材料的方法，以提高材料检测的效率。 用于检测调节VRK1活性的材料的方法包括：将组蛋白H3作为候选物质，VRK1和VRK1的底物进行处理的步骤; 测定组蛋白H3磷酸化的步骤; 以及比较组蛋白H3处理候选物质与未处理组蛋白H3之间的磷酸化程度的步骤。测定磷酸化的步骤包括：将反应蛋白与硝酸纤维素膜连接的步骤; 以及使用组蛋白H3-磷酸化的Thr3和Ser10或特异性识别组蛋白H3-磷酸化的Thr3和Ser10的免疫抗体来测定组蛋白H3的磷酸化的步骤。

公开(公告)号：KR101015969B1

公开(公告)日：2011-02-23

申请号：KR1020070135614

申请日：2007-12-21

Applicant: 포항공과대학교 산학협력단

Inventor： 김경태 ,  박도영 ,  강태홍

IPC: G01N33/574 ,  G01N33/68 ,  G01N33/53 ,  G01N33/48

Abstract: 본 발명은 백시니아-관련 카이네이즈 1 (vaccinia-related kinase 1, VRK1)의 활성조절물질을 발굴하는 데 있어서, VRK1의 특이적 인산화 부위를 밝힘으로써, 보다 효율적으로 VRK1의 활성조절물질을 탐색하는 방법에 관한 것이다. 이와 같이 검색된 VRK1의 활성조절물질은 항암제, 세포성장촉진제 등으로서 유용하다.