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公开(公告)号：WO2011071207A1
公开(公告)日：2011-06-16
申请号：PCT/KR2009/007603
申请日：2009-12-18
CPC classification number: C12N15/8243 , A23L7/196 , A23L33/105
Abstract: 본 발명은 벼의 OsNAS2 유전자 또는 OsNAS3 유전자의 활성화에 의해 미량 원소의 함량이 야생형에 비해 증가된 형질전환 식물체 및 상기 형질전환 식물체로부터 생성된 산물을 함유하는 기능성 식품에 관한 것이다.
Abstract translation: 本发明涉及通过激活OsNAS2基因或OsNAS3基因而与野生型相比微量元素含量增加的转基因水稻植物，以及包含由转基因植物制成的产品的功能性食品。
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公开(公告)号：KR1020080056395A
公开(公告)日：2008-06-23
申请号：KR1020060129240
申请日：2006-12-18
Applicant: 경희대학교 산학협력단 , 포항공과대학교 산학협력단
CPC classification number: C12N15/8245 , C12N9/1051 , C12Q1/6895 , C12Y204/01021
Abstract: A method for modifying endosperm starch property is provided to obtain a rice with a small, round and loose-structured starch granule of endosperm, thereby separating an outer layer from the rice being easy and saving energy for a process through heat-treatment. To prepare a rice with decreased starch crystallinity of endosperm compared to a wild-type rice, decreased starch chain having a starch polymerization degree(DP) of 6-8, 16-20 and at least 30, increased starch chain having a DP of 9-15 and 22-29, and 1-5 deg.C decreased gelatinization initiation temperature, a function-lost mutant OsSSIIIa/Flo5-1 prepared by inserting T-DNA into a starch synthase gene OsSSIIIa of Dongjin rice is used.
Abstract translation: 提供了一种改良胚乳淀粉性质的方法，以获得具有小，圆形和松散结构的胚乳淀粉颗粒的大米，从而使外层与米分离容易，并通过热处理节省了工艺的能量。 为了制备与野生型水稻相比淀粉结晶度降低的稻米，淀粉链的淀粉聚合度（DP）为6-8,16-20和至少30的淀粉链增加，DP为9的淀粉链 -15和22-29，1-5℃降低凝胶化起始温度，使用通过将T-DNA插入东金水稻的淀粉合成酶基因OsSSIIIa制备的功能缺失突变体OsSSIIIa / Flo5-1。
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公开(公告)号：KR1020110065863A
公开(公告)日：2011-06-16
申请号：KR1020090122543
申请日：2009-12-10
Applicant: 포항공과대학교 산학협력단
CPC classification number: C12N15/8243 , A23L7/196 , A23L33/105
Abstract: PURPOSE: A transgenic plant with enhanced trace element content by OsNAS2 gene activation is provided to grow the plants under the condition of lack heavy metal or excessive heave metal. CONSTITUTION: A plant with enhanced trace element content of iron, zinc, or copper has overexpressed OsNAS2 gene. The plant is Oriza sativa, barley, or corn. The expression of OsNAS2 is increases by insertion of an enhancer at the upstream or downstream of OsNAS2 gene. A method for preparing the plant with enhanced trace element content comprises: a step of introducing a structure containing a foreign enhancer into a plant, and a step of collecting a plant in which OsNAS2 gene is overexpressed.
Abstract translation: 目的：提供通过OsNAS2基因激活提高微量元素含量的转基因植物，在缺乏重金属或过度沉积金属的条件下生长植物。 构成：具有铁，锌或铜的微量元素含量增加的植物已经过量表达OsNAS2基因。 该植物是玉蜀黍，大麦或玉米。 通过在OsNAS2基因的上游或下游插入增强子，OsNAS2的表达增加。 制备具有增强的微量元素含量的植物的方法包括：将含有外源增强子的结构引入植物的步骤，以及收集OsNAS2基因过表达的植物的步骤。
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4.发明授权벼 ＯｓＡＧＰＬ１ 유전자, ＯｓＡＧＰＬ３ 유전자 또는ＯｓＡＧＰＬ４ 유전자 및 상기 유전자로 형질전환된 식물 失效RICE OSAGPL1基因，OSAGPL3基因或OSAGPL4基因和植物与其相互转化
公开(公告)号：KR100780486B1
公开(公告)日：2007-11-28
申请号：KR1020060129543
申请日：2006-12-18
Applicant: 경희대학교 산학협력단 , 포항공과대학교 산학협력단
Abstract: Each of genes, (OsAGP)L1, OsAGPL3 and OsAGPL4, regarding an ADP-glucose pyrophosphorylase derived from Oryza sativa L. which are three large subunit proteins for controlling the synthesis of starch is provided which is specifically located at a plastid in Oryza sativa L. cell. A gene encoding a large subunit of OsAGP is selected from the group consisting of an OsAGPL3 gene having a sequence of SEQ ID : NO. 2 and an OsAGPL4 gene having a sequence of SEQ ID : NO. 3, wherein the coding protein of each of the gene is specifically located at a plastid in a rice cell. A transgenic Oryza sativa L. plant is transformed by a recombinant plant expression vector including the gene. A method for preparing a transgenic Oryza sativa L. plant comprises the steps of: (a) transforming an Oryza sativa L. plant cell using the vector; and (b) redifferentiating the Oryza sativa L. plant cell into an Oryza sativa L. plant. A method for targeting a protein selected from the group consisting of OsAGPL3 and OsAGPL4 into a specific position in an Oryza sativa L. cell comprises a step of transforming an Oryza sativa L. plant cell using the vector, wherein the specific location is a plastid. A starch is isolated from the transgenic Oryza sativa L. plant prepared by the method.
Abstract translation: 关于作为三种用于控制淀粉合成的大的亚基蛋白的水稻的ADP-葡萄糖焦磷酸化酶基因（OsAGP）L1，OsAGPL3和OsAGPL4，其提供了特异性地位于水稻的质体 。 细胞。 编码OsAGP的大亚基的基因选自OsAGPL3基因，其具有SEQ ID NO： 2和具有SEQ ID NO：1的序列的OsAGPL4基因。 3，其中每个基因的编码蛋白特异性位于稻细胞中的质体。 通过包含该基因的重组植物表达载体转化转基因水稻L.植物。 一种制备转基因水稻植物的方法包括以下步骤：（a）使用载体转化水稻植物细胞; 和（b）将Oryza sativa L.植物细胞重新分配到Oryza sativa L.植物中。 将选自OsAGPL3和OsAGPL4的蛋白质定位在水稻L.细胞中的特定位置的方法包括使用载体转化水稻植物细胞的步骤，其中特定位置是质体。 从通过该方法制备的转基因水稻植物中分离淀粉。
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公开(公告)号：KR1020100085752A
公开(公告)日：2010-07-29
申请号：KR1020090005210
申请日：2009-01-21
Applicant: 칭하이 유니버시티 , 포항공과대학교 산학협력단
CPC classification number: C12N1/14 , C12N9/2437 , C12N9/248 , C12P7/02 , C12P7/649 , C12R1/645 , Y02E50/13
Abstract: PURPOSE: A novel Geotrichum sp. CGMCC 2711 which produces enzyme for lyzing polysaccharide is provided to decompose polysaccharide and to produce biofuel. CONSTITUTION: A Geotrichum sp. CGMCC 2711 produces an enzyme decomposing polysaccharide. The enzyme lyses xyloglucan or xylan polysaccharide. The xyloglucan is derived from Phaeocystis sp. The xylan is water-soluble xylan derived from Fagus engleriana Seemen. The enzyme has activity at pH 5.0-5.5 or 7.5-8.0 and 55-75°C. The enzyme contains Ca^2+ as a coenzyme.
Abstract translation: 目的： 提供产生用于溶解多糖的酶的CGMCC 2711以分解多糖并生产生物燃料。 构成：地精 CGMCC 2711产生酶分解多糖。 该酶裂解木葡聚糖或木聚糖多糖。 木葡聚糖衍生自Phaeocystis sp。 木聚糖是来自西葫芦（Fagus engleriana Seemen）的水溶性木聚糖。 该酶具有pH 5.0-5.5或7.5-8.0和55-75℃的活性。 该酶含有作为辅酶的Ca 2+。
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Patent Agency Ranking
公开(公告)号：KR1020100042459A
公开(公告)日：2010-04-26
申请号：KR1020080101614
申请日：2008-10-16
Applicant: 포항공과대학교 산학협력단
Abstract: PURPOSE: A transgenic rice plant in which small amount of element is increased is provided to enhance iron or zinc content and increase agricultural and nutritional benefit. CONSTITUTION: A plant in which small amount of element is enhanced is obtained by increasing expression of OsNAS3 gene. The element is iron, zinc, or copper. Plants are rice, barley or corn. The increase of OsNAS3 gene is performed by inserting an enhancer to upstream or downstream of the OsNAS3 gene. The plant is rice plant of deposit number KACC 98004P or KACC 98005P.
Abstract translation: 目的：提供少量元素增加的转基因水稻植物，以提高铁或锌含量，增加农业和营养利益。 构成：通过增加OsNAS3基因的表达获得少量元素增强的植物。 元素是铁，锌或铜。 植物是水稻，大麦或玉米。 通过在OsNAS3基因的上游或下游插入增强子来进行OsNAS3基因的增加。 该植物是KACC 98004P或KACC 98005P的保藏号为水稻的植物。
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公开(公告)号：KR100877837B1
公开(公告)日：2009-01-12
申请号：KR1020060129240
申请日：2006-12-18
Applicant: 경희대학교 산학협력단 , 포항공과대학교 산학협력단
Abstract: 본 발명은 자포니카 품종인 동진벼의 전분합성효소 유전자
OsSS
Ⅲa 에 T-DNA를 삽입한 기능상실 돌연변이체
OsSSⅢa/
Flo5
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1 를 이용하여, 야생형에 비해 쌀 배유의 전분결정성이 감소되며, 전분 중합도(DP) 6~8, 16~20 및 30 이상의 전분 사슬은 감소하고 DP 9~15 및 22~29는 증가하며, 호화개시온도가 1~5℃ 감소되는 것을 특징으로 하는 배유의 전분과립이 작고 둥글며 느슨한 구조로 형성된 쌀을 제조하는 방법에 관한 것으로, 이런 쌀은 지방질 등이 있는 외층분리가 용이하여 청주 등을 만들 때 적합하며 열처리를 통한 가공에 에너지 절약 효과가 있다.
동진벼, 전분합성효소 유전자(OsSSⅢa), 돌연변이체(OsSSⅢa/Flo5-1), 전분입자-
公开(公告)号：KR100836826B1
公开(公告)日：2008-06-10
申请号：KR1020060129246
申请日：2006-12-18
Applicant: 경희대학교 산학협력단 , 포항공과대학교 산학협력단
Abstract: A method for preparing a rice with enhanced processing suitability is provided to obtain the rice with a small, round and loose-structured starch granule of endosperm, thereby separation of an outer layer from the rice being easy and saving energy for a process through heat-treatment. A method for preparing a rice with enhanced processing suitability comprises the steps of: (a) inserting pGA272 of T-DNA into a starch synthase gene OsSSIIIa of Hwayoung rice to prepare a function-lost mutant; and (b) subjecting the mutant to PCR using a primer of SEQ ID : NOs. 1, 4, and 5 to select Hwayoung rice OsSSIIIa/flo5-2(Oryza sativa starch synthaseIIIa/floury5-2), wherein the rice has decreased starch crystallinity of endosperm compared with a wild-type rice, decreased starch chain having a starch polymerization degree(DP) of 6-8, 16-20 and at least 30, increased starch chain having a DP of 9-15 and 22-29, and 1-5 deg.C decreased gelatinization initiation temperature.
Abstract translation: 提供了一种具有增强的加工适应性的制备米的方法以获得具有小，圆形和松散结构的胚乳淀粉颗粒的米，从而使外层与米分离容易并且通过热处理节省了对于加工过程的能量， 治疗。 一种具有增强的加工适应性的制备水稻的方法包括以下步骤：（a）将T-DNA的pGA272插入Hwayoung水稻的淀粉合酶基因OsSSIIIa中以制备功能缺失型突变体; 和（b）使用SEQ ID NO：的引物对突变体进行PCR。 1，4和5选择Hwayoung rice OsSSIIIa / flo5-2（Oryza sativa淀粉合酶IIIa / floury5-2），其中与野生型水稻相比，稻米淀粉结晶度降低，淀粉链淀粉聚合降低 6-8,16-20度和（至少30）度的DP（DP）为9-15和22-29的DP增加的淀粉链和1-5℃的糊化起始温度降低。
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公开(公告)号：KR101255336B1
公开(公告)日：2013-04-16
申请号：KR1020090122543
申请日：2009-12-10
Applicant: 포항공과대학교 산학협력단
CPC classification number: C12N15/8243 , A23L7/196 , A23L33/105
Abstract: 본 발명은 벼의
OsNAS2 유전자의 활성화에 의해 미량 원소의 함량이 야생형에 비해 증가된 형질전환 식물체 및 상기 형질전환 식물체로부터 생성된 산물을 함유하는 기능성 식품에 관한 것이다.
벼, OsNAS2, 철-
公开(公告)号：KR101156572B1
公开(公告)日：2012-06-20
申请号：KR1020080101614
申请日：2008-10-16
Applicant: 포항공과대학교 산학협력단
Abstract: 본 발명은 벼의
OsNAS3 유전자의 활성화에 의해 미량 원소의 함량이 야생형에 비해 증가된 형질전환 식물체 및 상기 형질전환 식물체로부터 생성된 산물을 함유하는 기능성 식품에 관한 것이다.
벼, OsNAS3, 철
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