公开(公告)号：KR100804713B1

公开(公告)日：2008-02-18

申请号：KR1020060105908

申请日：2006-10-30

Applicant: 포항공과대학교 산학협력단

Inventor： 차형준 ,  서정현 ,  이혜연 ,  이봉국 ,  아다치쿄이치 ,  토모지가와이

IPC: C07H5/10

CPC classification number: A61K49/00 ,  C07H15/18

Abstract: A method for immobilizing saccharide on the surface of a substrate is provided to immobilize directly the saccharide on the surface of a solid substrate without changing the structure of the saccharide and is applied to various saccharides. A method for immobilizing saccharide on the surface of a substrate such as gold comprises the steps of: (a) reacting an aldehyde of the saccharide selected from the group consisting of reducing monosaccharide, oligosaccharide having a terminal reducing sugar, and polysaccharide with an amine group of an amine compound represented by NH2-R-SH(where R is CC30-50 alkylene, C6-30 aryl or C6-30 aryl including C10-30 alkylene) to prepare a thiol group bound saccharide; and coating the thiol group bound saccharide to the surface of the substrate to bind the thio to the substrate surface, where when the saccharide is a monosaccharide, the reaction is done at a temperature of 20-90 deg.C for 0.5-2 hours, and when the saccharide is a polysaccharide, the reaction is done at a temperature of 30-90 deg.C for 0.5-2 hours. The method further comprises a step of reducing the thiol bound saccharide.

Abstract translation: 提供了将糖固定在基材表面上的方法，用于将糖直接固定在固体基质的表面上，而不改变糖的结构并施加到各种糖上。 使糖类固定在基底如金的表面上的方法包括以下步骤：（a）将选自还原性单糖，末端还原糖的寡糖与多糖的糖类与胺基反应 由NH2-R-SH（其中R是CC30-50亚烷基，C6-30芳基或包括C10-30亚烷基的C6-30芳基）表示的胺化合物以制备巯基结合的糖; 并将硫醇结合的糖包被到底物的表面以将硫结合到底物表面，其中当糖是单糖时，反应在20-90℃的温度下进行0.5-2小时， 当糖是多糖时，反应在30-90℃的温度下进行0.5-2小时。 该方法还包括还原硫醇结合的糖的步骤。