公开(公告)号：KR1020100051036A

公开(公告)日：2010-05-14

申请号：KR1020090107145

申请日：2009-11-06

Applicant: 포항공과대학교 산학협력단

Inventor： 최동훈 ,  양세환 ,  성영철

IPC: C12N5/0775 ,  C12N5/074 ,  C12Q1/24 ,  G01N33/50 ,  C12N5/00 ,  C12N5/0783

CPC classification number: C12N5/0663 ,  A61K2035/124 ,  C12N5/00 ,  C12N5/0018 ,  C12N5/0607 ,  C12N5/0637 ,  C12N5/0638 ,  C12N5/0665 ,  C12N5/0667 ,  C12Q1/24 ,  G01N33/5073 ,  G01N2333/7055

Abstract: PURPOSE: An activation method of an antigen-specific autologous CD8 T cell using alpha-galactosylceramide is provided to minimize side effects by processing the activation in vitro, and to use the activated CD8 T as an anti-tumor vaccine composition. CONSTITUTION: An activation method of an antigen-specific autologous CD8 T cell comprises the following steps: dividing CD8 T cells from a biological material; mixing the CD8 T cells with an antigen presenting cell(APC); processing the mixed cells with alpha-galactosylceramide or its derivative and cultivating; and separating the activated CD8 T cell from the cultivated cells. The antigen-specific autologous CD8 T cell is combined with the alpha-galactosylceramide or its derivative to transfer the alpha-galactosylceramide or its derivative into a human body.

Abstract translation: 目的：提供使用α-半乳糖神经酰胺的抗原特异性自体CD8T细胞的活化方法，以通过在体外处理活化来最小化副作用，并使用活化的CD8T作为抗肿瘤疫苗组合物。 构成：抗原特异性自体CD8T细胞的活化方法包括以下步骤：从生物材料中分离CD8T细胞; 将CD8T细胞与抗原呈递细胞（APC）混合; 用α-半乳糖神经酰胺或其衍生物处理混合细胞并培养; 并将活化的CD8T细胞与培养细胞分离。 将抗原特异性自体CD8T细胞与α-半乳糖神经酰胺或其衍生物结合以将α-半乳糖神经酰胺或其衍生物转移到人体内。