Abstract:
본 발명은 병풀( Centella asiatica(L) Urb )로부터의 생리활성 물질의 추출 및 분리 방법에 관한 것으로, 보다 자세하게는 인체에 무해하고 환경친화적인 용매인 아임계수를 이용하여 상처치유 촉진, 우수한 세포증식, 콜라겐 합성 등의 효과를 갖는 물질인 아시아틱산과 아시아티코사이드를 입자 상태로 추출하여 분리하는 방법에 관한 것이다. 병풀, 아시아틱산, 아시아티코사이드, 아임계수, 추출, 분리, 여과
Abstract:
PURPOSE: A method for extracting and isolating a physiological material from Centella asiatica is provided to promote wound healing, cell proliferation, and collagen synthesis. CONSTITUTION: A method for extracting and isolating asiatic acid and asiaticoside comprises: a step of injecting Centella asiatica in a reactor to contact with water; a step of increasing temperature and pressure of water to make to subcritical water; a step of contacting the Centella asiatica and subcritical water to prepare Centella asiatica extract; a step of lowering the temperature and pressure of the subcritical water to collect the Centella asiatica extract; and a step of filtering the Centella asiatica extract to obtain asiatic acid and asiaticoside.
Abstract:
Cephalosporins (I)[R1=H, CHO; X=(II) or O2CCH3 were prepd.. Thus, 7- amino-3-(1-methyl-1H-tetrazol-5-ylthiomethyl)-3-cephem-4carboxylic acid and monotrimethylacetamide in ethylacetate was heated for 50min at 50≦̸C, cooled to >=-15≦̸C, and then reacted with a mixt. of D- mandelic acid formate ester and diphenyl-2-pyridone1-phosphate in triethylamine for 150min at 50≦̸C with stirring. The reaction mixt. was extd. with ethylacetate/H2O (1:1) and filtered. The aq. phase extd. with ethylacetate and org. phase pooled, washed with 5% HNO3 and satd. NaCl over 2 times, resptly.. The org. phase was dried on MgSO4, filtered, vacuum-evapd, and crystallized with ethylacetate/n- hexane.