公开(公告)号：KR100303611B1

公开(公告)日：2001-09-24

申请号：KR1019990027167

申请日：1999-07-07

Applicant: 한국과학기술연구원

Inventor： 김병홍 ,  장인섭 ,  현문식 ,  김형주 ,  박형수

IPC: G01N33/18

CPC classification number: G01N33/1866 ,  G01N33/1806

Abstract: 본발명에따라, 혐기적조건에서매개체없는생물연료전지를사용하여전기화학적인방법으로시료속의유기물농도또는 BOD를측정할수 있는바이오센서가제공된다. BOD 측정용바이오센서에사용되는생물연료전지의전기화학적활성세균은생물연료전지의운전과정에서농화배양된폐수및 슬러지속에포함된활성세균이사용된다. 따라서, 본발명의 BOD 바이오센서는인위적인미생물의첨가없이운전될수 있으며폐수에따라적절한세균의활성이유지될수 있다. 바이오센서용생물연료전지는 6개월이상안정적으로작동할수 있다.

公开(公告)号：KR1020010009030A

公开(公告)日：2001-02-05

申请号：KR1019990027167

申请日：1999-07-07

Applicant: 한국과학기술연구원

Inventor： 김병홍 ,  장인섭 ,  현문식 ,  김형주 ,  박형수

IPC: G01N33/18

CPC classification number: G01N33/1866 ,  G01N33/1806

Abstract: PURPOSE: Provided are a method of electrochemical dense culture of a microorganism and a biosensor for analyzing organic material and BOD. The biosensor for BOD analysis can be operated without addition of the microorganism, can maintain an optimal bacterial activity depending on sewage and can determined BOD of the sewage successively by using activated bacteria contained in the sewage and sludge as a biological fuel cell. Also, the biological fuel cell is stably operated for up to 6 months. CONSTITUTION: The biosensor for BOD analysis comprises a measuring portion, a detecting portion of current, a monitoring portion recording detected current change, and optionally, a potentiostat, wherein the measuring portion is the biological fuel cell without additional mediator or transducer comprising an anode, a cathode, a conduction mediator of the anode and the cathode, and an anion switch membrane between the two pole, and wherein the cathode part comprises a sample containing electrochemically activated bacteria.

Abstract translation: 目的：提供微生物电化学致密培养的方法和用于分析有机物和BOD的生物传感器。 用于BOD分析的生物传感器可以在不添加微生物的情况下操作，可以根据污水保持最佳的细菌活性，并且可以通过使用污水和污泥中包含的活性细菌作为生物燃料电池，连续测定污水的BOD。 此外，生物燃料电池稳定运行长达6个月。 构成：用于BOD分析的生物传感器包括测量部分，电流的检测部分，记录检测到的电流变化的监测部分和可选的恒电位仪，其中测量部分是没有附加介体或换能器的生物燃料电池，其包括阳极， 阴极，阳极和阴极的传导介质和两极之间的阴离子交换膜，其中阴极部分包含含有电化学活化的细菌的样品。

公开(公告)号：KR100890364B1

公开(公告)日：2009-03-25

申请号：KR1020070027532

申请日：2007-03-21

Applicant: 한국과학기술연구원

Inventor： 최용수 ,  홍석원 ,  이상협 ,  김형주 ,  최창호 ,  정봉근

IPC: G01N27/00 ,  G01N21/33 ,  G01N33/20

CPC classification number: G01N27/42

Abstract: 본 발명은 액체 시료 속 주석 화합물의 양을 연속적·자동적으로 정량할 수 있는 무인 분석 장치와 이를 이용한 주석 화합물의 정량 방법에 대한 발명이다. 본 발명의 무인 분석 장치에서는 액체 시료에 산을 투여한 뒤 선택적인 자외선 조사를 통하여 시료 속에 유기주석 화합물이 존재하는 경우는 이를 무기주석으로 변환시키고, 이 변환된 무기주석을 전기화학적 방법을 사용하여 정량하게 되는 것이 특징이다. 본 발명에 따르면 유기주석 화합물 상태에서는 측정이 까다로운 주석을 측정이 가능한 무기주석 화합물로 바꾸어 전기화학적인 방법으로 측정하여, 액체 시료 내에 원래 포함된 미량의 유기주석 및 무기주석 화합물의 농도를 각각 측정할 수 있다. 무엇보다도 무인 자동화 분석 장치를 구성하여 연속적으로 시료를 측정할 수 있으며, 부가적인 조작을 필요로 하지 않기 때문에 인력을 절감할 수 있는 효과가 있다. 본 발명에서는 아울러 이러한 무인 자동화 분석 장치를 이용한, 액체 시료 속 주석 화합물의 정량 방법도 제공한다. 또 유기주석 화합물을 무기주석 화합물로 변환하는 무인 자동화 장치와 이를 이용한 유기주석 화합물의 무기주석 화합물로의 변환 방법도 제공한다.
유기주석, 무인 자동화 분석 장치, 액체 시료, 벗김 전압-전류법

公开(公告)号：KR1020030038240A

公开(公告)日：2003-05-16

申请号：KR1020010069947

申请日：2001-11-10

Applicant: 한국과학기술연구원

Inventor： 김병홍 ,  장인섭 ,  김형주 ,  김미아

IPC: G01N33/18

Abstract: PURPOSE: A method for measuring low concentration of BOD by using a microbial fuel cell type BOD apparatus using low nutritional requirement and electrochemically active microorganisms is provided, thereby stably and correctly measuring low density of BOD. CONSTITUTION: A microbial fuel cell type BOD apparatus using low nutritional requirement and electrochemically active microorganisms comprises a BOD measuring portion, an electronic current detecting portion, and a recording portion recording a change of the electronic current detected, wherein the BOD measuring portion comprises an anode portion, a cathode portion, a conduction medium and an ion exchange membrane between the anode and cathode. The method for measuring low concentration of BOD comprises adding waste water as a source of low nutritional requirement and electrochemically active microorganisms and glucose and glutamic acid as substrates into the cathode portion and culturing them.

Abstract translation: 目的：提供一种通过使用低营养需要的微生物燃料电池型BOD装置和电化学活性微生物测量低浓度BOD的方法，从而稳定且正确地测量低密度的BOD。 构成：使用低营养需求的微生物燃料电池BOD装置和电化学活性微生物包括BOD测量部分，电子电流检测部分和记录检测到的电子电流变化的记录部分，其中BOD测量部分包括阳极 阴极部分，导电介质和阳极和阴极之间的离子交换膜。 用于测量低浓度BOD的方法包括将作为低营养需求的来源的废水和作为底物的电化学活性微生物和葡萄糖和谷氨酸添加到阴极部分并进行培养。

公开(公告)号：KR1020020036292A

公开(公告)日：2002-05-16

申请号：KR1020000066424

申请日：2000-11-09

Applicant: 주식회사 코비 ,  한국과학기술연구원

Inventor： 조병원 ,  현문식 ,  김형주 ,  김영필 ,  김병홍 ,  추용엽

IPC: G01N27/48

Abstract: PURPOSE: An electrochemical multi-stripping analysis method and system for detecting and measuring heavy metal ion is provided to suppress interference effect of ions and achieve improved accuracy for measurement, while preventing environmental contamination. CONSTITUTION: A multi-stripping analysis system comprises an electrochemical cell(2) where an electrochemical reaction like plating or stripping occurs; a potentiostat/galvanostat(1) for applying electric potential or current to the gap between electrodes and detecting response resulted from the application of electric potential or current; and a computer for controlling each unit and analyzing result. The electrochemical cell includes multiple electrodes(WE) as a working electrode; a reference electrode(RE) for controlling and measuring electric potential of the working electrode; and a counter electrode(CE) having a wide area so as to allow the working electrode to have a smooth current flow.

Abstract translation: 目的：提供一种用于检测和测量重金属离子的电化学多剥离分析方法和系统，以抑制离子的干扰作用，提高测量精度，同时防止环境污染。 构成：多剥离分析系统包括电化学电池（2），其中发生如电镀或剥离的电化学反应; 用于向电极之间的间隙施加电位或电流的电位器/恒电流仪（1），并检测由施加电位或电流引起的响应; 以及用于控制每个单元并分析结果的计算机。 电化学电池包括多个电极（WE）作为工作电极; 用于控制和测量工作电极的电位的参考电极（RE）; 以及具有广泛面积的对电极（CE），以允许工作电极具有平滑的电流流动。

公开(公告)号：KR100332932B1

公开(公告)日：2002-04-20

申请号：KR1019990027168

申请日：1999-07-07

Applicant: 한국과학기술연구원

Inventor： 김병홍 ,  장인섭 ,  현문식 ,  김형주 ,  박형수

IPC: C02F3/00

CPC classification number: C02F3/30 ,  C02F3/00 ,  C02F3/005 ,  C02F11/00 ,  C02F2103/32 ,  C02F2201/46115 ,  H01M8/16 ,  Y02E60/527 ,  Y02W10/15

Abstract: 본발명은폐수를연료로한 생물연료전지를제공한다. 본발명에사용되는폐수와활성슬러지내의전기화학적활성미생물은폐수내의유기물을산화하여발생하는전자를세포외부로방출하여직접적으로전극에전달하여전류를발생시키며, 폐수를정화시킬수 있다. 본발명에따른전기화학적활성을지닌세균을이용한생물연료전지에서발생하는전류는최대 0.22 mA의전기에너지를발생하였고, 연료로사용한폐수의 CODcr을최대 1900 ppm에서 55 ppm까지감소시켰다. 연료전지의효율은폐수종류와폐수농도에따라차이가있었다.

公开(公告)号：KR100330687B1

公开(公告)日：2002-04-03

申请号：KR1019990057376

申请日：1999-12-14

Applicant: 한국과학기술연구원

Inventor： 김병홍 ,  장인섭 ,  현문식 ,  김형주 ,  박형수

IPC: C02F3/34

Abstract: 본발명은황산염환원세균을이용하여 6가크롬등의중금속함유폐수를처리하는방법에있어서, 불용성의고형유기물 (유기성담체)가충전된충전층반응기를이용하는폐수처리방법에관한것이다. 또한, 중금속의처리효과를높이기위해서, 유기수용성폐수를유기성담체와혼합하거나또는별도로동시에공급할수 있는데, 이때 유기성폐수에서유래하는유기물이처리수로배출되지않도록운전함으로써동시에두 가지폐수의처리가가능하다. 본발명의방법은다른중금속이함유된폐수에도적용가능하다.

公开(公告)号：KR1020010009031A

公开(公告)日：2001-02-05

申请号：KR1019990027168

申请日：1999-07-07

Applicant: 한국과학기술연구원

Inventor： 김병홍 ,  장인섭 ,  현문식 ,  김형주 ,  박형수

IPC: C02F3/00

CPC classification number: C02F3/30 ,  C02F3/00 ,  C02F3/005 ,  C02F11/00 ,  C02F2103/32 ,  C02F2201/46115 ,  H01M8/16 ,  Y02E60/527 ,  Y02W10/15

Abstract: PURPOSE: Provided is a bio-electric cell that organic material of wastewater is used as fuel and microorganisms of activated sludge is used as catalyst. Wastewater and activated sludge are filled around a cathode. At the cathode, microorganisms of wastewater or activated sludge are cultivated in an anaerobic condition, so only microorganisms, which use the cathode as electron acceptor, are cultivated selectively. Generally, microorganisms are covered with lipid film being nonconductive, so electron exchange is not occurred directly between microorganisms and electrode. Therefore, phosphate buffer solution is used as an electronic conductor in the bio-electric cell. Consequently, electrochemically activated microorganisms oxidize organic material of wastewater and electrons are produced by oxidation of organic material. The electrons are discharged to the exterior of cell of the microorganisms and are transferred to the cathode. Then, electric current is generated and wastewater is purified by oxidation of organic material. CONSTITUTION: The bio-electric cell is comprising by follow parts: graphite felts used as cathode and anode; a cation exchange resin to minimize resistance of the bio-electric cell; phosphate buffer solution for electronic conduction; an anode being aerobic condition; a cathode being anaerobic condition.

Abstract translation: 目的：提供废水有机物作为燃料的生物电池，活性污泥的微生物用作催化剂。 废水和活性污泥填充在阴极周围。 在阴极处，废水或活性污泥的微生物在厌氧条件下培养，所以只有使用阴极作为电子受体的微生物被选择性培养。 通常，微生物被不导电的脂质膜覆盖，因此微生物和电极之间不直接发生电子交换。 因此，磷酸盐缓冲溶液用作生物电池中的电子导体。 因此，电化学活化的微生物氧化废水的有机物质，电子通过有机材料的氧化产生。 电子被排出到微生物细胞的外部并被转移到阴极。 然后，产生电流，并通过有机材料的氧化净化废水。 构成：生物电池由以下部分组成：用作阴极和阳极的石墨毡; 阳离子交换树脂，以最小化生物电池的电阻; 用于电子传导的磷酸盐缓冲溶液; 阳极是有氧条件; 阴极是无氧条件。

公开(公告)号：KR1020080086002A

公开(公告)日：2008-09-25

申请号：KR1020070027532

申请日：2007-03-21

Applicant: 한국과학기술연구원

Inventor： 최용수 ,  홍석원 ,  이상협 ,  김형주 ,  최창호 ,  정봉근

IPC: G01N27/00 ,  G01N21/33 ,  G01N33/20

CPC classification number: G01N27/42

Abstract: A device for analyzing tin compound in a liquid sample and a tin analyzing method using the same are provided to reduce pre-treatment time by changing an organic tin compound into an inorganic tin compound so as to measure the tin compound with an electrochemical method. A device for analyzing tin compound in a liquid sample consecutively measures the concentration of an inorganic or organic tin compound in the liquid sample through stripping voltammetery, and has a sample reservoir, an acid injector, a reactor and a detector. The sample reservoir stores the liquid sample. The acid injector injects acid to the liquid sample to adjust acidity for a set level. The reactor conducts an electrochemical measurement on the acid-injected tin sample. The detector detects the electrochemical signal sent from the reactor.

Abstract translation: 提供一种用于分析液体样品中锡化合物的装置和使用其的锡分析方法，以通过将有机锡化合物改变成无机锡化合物以通过电化学方法测量锡化合物来减少预处理时间。 用于分析液体样品中锡化合物的装置通过溶出伏安法连续测量液体样品中无机或有机锡化合物的浓度，并具有样品储存器，酸注射器，反应器和检测器。 样品储存器存储液体样品。 酸性注射器将酸注入液体样品中以将酸度调节至一定水平。 反应器对酸注入的锡样品进行电化学测量。 检测器检测从反应器发送的电化学信号。

公开(公告)号：KR100435817B1

公开(公告)日：2004-06-12

申请号：KR1020010069947

申请日：2001-11-10

Applicant: 한국과학기술연구원

Inventor： 김병홍 ,  장인섭 ,  김형주 ,  김미아

IPC: G01N33/18

Abstract: PURPOSE: A method for measuring low concentration of BOD by using a microbial fuel cell type BOD apparatus using low nutritional requirement and electrochemically active microorganisms is provided, thereby stably and correctly measuring low density of BOD. CONSTITUTION: A microbial fuel cell type BOD apparatus using low nutritional requirement and electrochemically active microorganisms comprises a BOD measuring portion, an electronic current detecting portion, and a recording portion recording a change of the electronic current detected, wherein the BOD measuring portion comprises an anode portion, a cathode portion, a conduction medium and an ion exchange membrane between the anode and cathode. The method for measuring low concentration of BOD comprises adding waste water as a source of low nutritional requirement and electrochemically active microorganisms and glucose and glutamic acid as substrates into the cathode portion and culturing them.

Abstract translation: 目的：提供一种通过使用低营养需求的微生物燃料电池型BOD装置和电化学活性微生物来测量低浓度BOD的方法，由此稳定且正确地测量BOD的低密度。 本发明公开了一种使用低营养需求和电化学活性微生物的微生物燃料电池型BOD装置，包括BOD测量部分，电子电流检测部分和记录检测到的电子电流变化的记录部分，其中BOD测量部分包括阳极 部分，阴极部分，传导介质和阳极与阴极之间的离子交换膜。 测量低浓度BOD的方法包括将作为低营养需要的来源的废水和电化学活性的微生物以及葡萄糖和谷氨酸作为底物加入到阴极部分并培养它们。