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1.发明公开
公开(公告)号：KR1020130010796A
公开(公告)日：2013-01-29
申请号：KR1020110071672
申请日：2011-07-19
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A method for differentiating stem cells into smooth muscle cells and a smooth muscle cell-scaffold complex for tissue engineering are provided to effectively differentiate human adipose-derived stem cells into the smooth muscle cells. CONSTITUTION: A method for differentiating stem cells into smooth muscle cells comprises a step of applying stress to a stem cell-inoculating elastic scaffold in a medium containing biochemical differentiation factor of TFG-beta-1, platelet-derived growth factor-BB(PDGF-BB), ascorbic acid, retinoic acid, or angiotensin II. The biochemical differentiation factor is retinoic acid. The elastic scaffold is formed of biodegradable polymers. The biodegradable polymer is a copolymer of lactic acid and carprolactone. A smooth muscle cell-scaffold complex for tissue engineering contains the differentiated smooth muscle cells for vascular disease treatment or wound healing.
Abstract translation: 目的：提供一种将干细胞分化为平滑肌细胞的方法和用于组织工程的平滑肌细胞 - 支架复合物，以有效区分人脂肪干细胞进入平滑肌细胞。 构成：将干细胞分化为平滑肌细胞的方法包括在含有TFG-β-1，血小板衍生生长因子-BB（PDGF-BB）生物化学分化因子的培养基中的干细胞接种弹性支架上施加应力的步骤， BB），抗坏血酸，视黄酸或血管紧张素II。 生化分化因子是视黄酸。 弹性支架由可生物降解的聚合物形成。 生物可降解聚合物是乳酸和己内酯的共聚物。 用于组织工程的平滑肌细胞 - 支架复合物包含用于血管疾病治疗或伤口愈合的分化的平滑肌细胞。
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Patent Agency Ranking
公开(公告)号：KR101344719B1
公开(公告)日：2013-12-26
申请号：KR1020110071672
申请日：2011-07-19
Applicant: 한국과학기술연구원
Abstract: 본발명은줄기세포가접종된탄성스캐폴드에응력(Strain)을가하면서줄기세포를평활근세포로분화시키는방법및 상기방법에의해분화된평활근세포를함유하는혈관질환또는창상치료를위한조직공학용평활근세포패치에관한것이다. 본발명은기계적응력과생화학인자의인간지방줄기세포의평활근세포분화에미치는시너지효과를증명하였고기계-활성조직공학에있어서줄기세포의유용한응용법을제공한다.
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公开(公告)号：KR101265492B1
公开(公告)日：2013-05-20
申请号：KR1020110107237
申请日：2011-10-19
Applicant: 한국과학기술연구원
CPC classification number: C12N5/069 , A61K35/28 , A61K35/30 , A61K35/44 , A61K35/51 , A61K35/545 , C12N2501/115 , C12N2506/1384 , C12N2513/00 , C12N2533/52 , C12N2533/54 , C12N2533/70
Abstract: 본발명은줄기세포를 3차원세포집합체(cell cluster) 형태로배양하여혈관세포로분화시키는방법및 생체내 혈관신생을위한상기 3차원세포집합체의용도에관한것이다. 구체적으로, 본발명은 1) 줄기세포를표면이소수성을띠는배양용기혹은성장인자가고정화된배양용기에접착시켜배양하는단계; 2) 배양된줄기세포의밀도가증가함에따라줄기세포가배양용기로부터탈착되어 3차원세포집합체를형성하는단계; 및 3) 3차원세포집합체형태로증식하면서줄기세포가혈관세포로분화되는단계를포함하는, 줄기세포를혈관세포로분화시키는방법및 상기방법에의해줄기세포로부터분화된혈관세포로구성된 3차원세포집합체를혈관신생을위한세포치료제로사용하는용도에관한것이다.
Abstract translation: 本发明涉及一种通过以三维细胞簇的形式培养它们并使用三维细胞簇进行血管发生来将干细胞分化为血管细胞的方法。 具体而言，本发明教导了将干细胞分化为血管细胞的方法，包括通过将干细胞粘附到具有疏水性的表面的培养板上或培养板上固定有生长因子培养干细胞，其中培养的干细胞 随着其密度增加而随培养板分离，形成三维细胞簇，并以三维细胞簇的形式生长，同时分化为血管细胞。 还公开了通过上述方法将由干细胞分化的血管细胞组成的三维细胞簇用作血管发生的细胞治疗剂。
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公开(公告)号：KR1020110129842A
公开(公告)日：2011-12-02
申请号：KR1020110107237
申请日：2011-10-19
Applicant: 한국과학기술연구원
CPC classification number: C12N5/069 , A61K35/28 , A61K35/30 , A61K35/44 , A61K35/51 , A61K35/545 , C12N2501/115 , C12N2506/1384 , C12N2513/00 , C12N2533/52 , C12N2533/54 , C12N2533/70
Abstract: PURPOSE: A method for differentiation of stem cells into vascular cells by culturing in a three-dimensional cell cluster is provided to form hypoxic state and to efficiently induce the differentiation. CONSTITUTION: A composition for cell therapy for treating vascular diseases or wound contains a cell cluster containing vascular cells differentiated from the stem cell and culture liquid thereof as an active ingredient. The vascular diseases are cardiovascular diseases, cerebrovascular diseases, or ischemic diseases. The stem cells are adipose stem cells, mesenchymal stem cells, bone marrow stem cells, umbilical stem cells, nervous stem cells, or pluripotent stem cells. A three-dimensional cell cluster is formed in a hypoxia condition therein.
Abstract translation: 目的：提供通过在三维细胞簇中培养将干细胞分化成血管细胞的方法，以形成缺氧状态并有效诱导分化。 构成：用于治疗血管疾病或伤口的细胞治疗用组合物含有含有作为活性成分的干细胞和培养液分化的血管细胞的细胞簇。 血管疾病是心血管疾病，脑血管疾病或缺血性疾病。 干细胞是脂肪干细胞，间充质干细胞，骨髓干细胞，脐带干细胞，神经干细胞或多能干细胞。 在其中的缺氧条件下形成三维细胞簇。
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公开(公告)号：KR1020110030030A
公开(公告)日：2011-03-23
申请号：KR1020090087959
申请日：2009-09-17
Applicant: 한국과학기술연구원
CPC classification number: C12M35/08 , C12N5/0653 , C12N15/66
Abstract: PURPOSE: A method for differentiating adipose stem cells to adipocytes using heparin-binding domain(HBD) is provided to induce cell adhesion and to effectively obtain a large amount of adipocytes. CONSTITUTION: A culture container is fixed on a heparin-binding domain(HBD) with an adhesion activity of adipose stem cells. The HBD is fixed in a recombinant protein form with a polypeptide linker by physical absorption. The polypeptide linker is maltose-binding protein(MBP), hydrophobin, or hydrophobic cell penetrating peptides(CPPs). The recombinant protein has an amino acid sequence of sequence number 2.
Abstract translation: 目的：提供使用肝素结合结构域（HBD）将脂肪干细胞分化为脂肪细胞的方法，以诱导细胞粘附并有效获得大量的脂肪细胞。 构成：将培养容器固定在具有脂肪干细胞粘附活性的肝素结合结构域（HBD）上。 通过物理吸收将HBD固定为具有多肽接头的重组蛋白形式。 多肽接头是麦芽糖结合蛋白（MBP），疏水蛋白或疏水细胞穿透肽（CPPs）。 重组蛋白具有序列号2的氨基酸序列。
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公开(公告)号：KR101171958B1
公开(公告)日：2012-08-08
申请号：KR1020090087959
申请日：2009-09-17
Applicant: 한국과학기술연구원
Abstract: 본 발명은 헤파린 결합 도메인(heparin-binding domain, HBD)을 이용한 지방줄기세포의 지방세포로의 분화방법에 관한 것으로서, 구체적으로 지방줄기세포(adipose-derived stem cells; ASCs)에 대한 접착활성을 갖는 HBP가 표면에 고정되어 있는 배양용기; 및 상기 배양용기에 지방줄기세포를 접종하여 지방줄기세포를 배양용기 표면에 접착된 상태로 배양하는 단계를 포함하는 지방줄기세포의 지방세포로의 분화방법에 관한 것이다. 본 발명에 따라 지방줄기세포에 대한 접착활성을 갖는 HBD를 배양용기에 고정시키면, HBD와 지방줄기세포의 상호작용에 의한 세포접착이 유도되어 지방줄기세포를 배양용기의 표면에 접착된 상태로 배양할 수 있어 지방세포로의 대량 분화를 효과적으로 유도할 수 있다. 따라서 본 발명은 지방줄기세포의 분화 및 증식 연구와, 조직공학 및 세포치료와 같은 재생 의료분야에 매우 유용하게 적용될 수 있다.
지방줄기세포, 지방세포, 세포접착, 헤파린-결합 도메인, 세포분화
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