公开(公告)号：KR100321304B1

公开(公告)日：2002-03-18

申请号：KR1019990013598

申请日：1999-04-16

Applicant: 한국과학기술연구원

Inventor： 김창진 ,  박해룡 ,  박동진

IPC: C12P1/06

Abstract: 본발명은테이코플라닌(teicoplanin)의정제방법에관한것으로서, 더욱상세하게는테이코플라닌생성균주의배양단계; 상기배양액의소수성흡착크로마토그래피단계; 그리고, 당-친화성크로마토그래피단계를포함하는테이코플라닌정제방법을그 특징으로하는것으로서, 종전의방법과비교하여그 과정이단순할뿐만아니라, 배양과정에서자가독성을감소시킴으로써그 수율도증가시킬수 있는효과가있다.

公开(公告)号：KR1020000066479A

公开(公告)日：2000-11-15

申请号：KR1019990013598

申请日：1999-04-16

Applicant: 한국과학기술연구원

Inventor： 김창진 ,  박해룡 ,  박동진

IPC: C12P1/06

Abstract: PURPOSE: Provided is a method for purifying teicoplanin having antimicrobial activity. Thereby, teicoplanin is prepared easily in high yield by decreasing autotoxicity. CONSTITUTION: A method for purifying teicoplanin comprises the following steps of; (a) culturing teicoplanin producing strain; (b) performing hydrophobic adsorption chromatography of the culture broth, wherein the used resin is preferably selected from XAD-16, HP-20, silicagel or activated carbon; and (c) performing sugar-affinity chromatography of the culture broth, wherein concanvalin A, lentil lectin or wheat germ lectin is immobilized to the resin. Preferably, the producing teicoplanin strain is Actinoplanes teichomyceticus. The method further comprises step of adding neutral resin such as XAD-16. HP-20, silicagel or active carbon, to decrease autotoxicity phenomenon by teicoplanin.

Abstract translation: 目的：提供具有抗菌活性的替考拉宁纯化方法。 因此，通过降低自身毒性，可以容易地制备替考拉宁。 构成：提纯替考拉宁的方法包括以下步骤： （a）培养替考拉宁生产菌株; （b）进行培养液的疏水吸附色谱法，其中所用的树脂优选选自XAD-16，HP-20，硅胶或活性炭; 和（c）对所述培养液进行糖亲和层析，其中将所述血清白蛋白A，扁豆凝集素或小麦胚芽凝集素固定在树脂上。 优选地，生产替考拉宁菌株是鸟氨酸激酶。 该方法还包括加入中性树脂如XAD-16的步骤。 HP-20，硅胶或活性炭，以减少替考拉宁的自身毒性现象。

公开(公告)号：KR100321305B1

公开(公告)日：2002-03-18

申请号：KR1019990013665

申请日：1999-04-17

Applicant: 한국과학기술연구원

Inventor： 김창진 ,  박해룡 ,  박동진

IPC: C12N1/20

Abstract: 본발명은변이주액티노플라네스테이코마이세티커스 () MSL 2211 및그로부터생산되는테이코플라닌에관한것으로서, 더욱상세하게는인위적돌연변이유도에의해제조되는변이주액티노플라네스테이코마이세티커스 MSL 2211 및최적화된배양조건에서그로부터제조되는테이코플라닌에관한것으로서, 본발명신규변이주및 최적화된배양조건을이용하는경우에는산업적으로테이코플라닌을대량생산할수 있는효과가있다.

公开(公告)号：KR1020000066501A

公开(公告)日：2000-11-15

申请号：KR1019990013665

申请日：1999-04-17

Applicant: 한국과학기술연구원

Inventor： 김창진 ,  박해룡 ,  박동진

IPC: C12N1/20

Abstract: PURPOSE: A mutant Actinoplanes teichomyceticus MSL 2211 is provided which mass produces teicoplanin under the optimum culture condition. Also, a preparation method of teicoplanin is provided. CONSTITUTION: An Actinoplanes teichomyceticus MSL 2211(KCTC 8926p) is characterized by showing a high productivity of teicoplanin. A culture medium of Actinoplanes teichomyceticus MSL 2211 is characterized by comprising 75-85 wt.% of saccharobiose, 10-20 wt.% of yeast extract, 2-5 wt.% of amino acid, 2.2-5 wt.% of inorganic salt. The saccharobiose is characterized by comprising a mannose. The amino acid is characterized by comprising asparagine. The inorganic salt is characterized by comprising magnesium 2-4 wt.% of sulfate, 0.1-0.5 wt.% of sodium chloride, 0.1-0.5 wt.% of calcium chloride.

Abstract translation: 目的：提供一种突变型肌动蛋白电纺霉素MSL 2211，其在最佳培养条件下大量生产替考拉宁。 另外，提供了替考拉宁的制备方法。 构成：一种激动剂鸟氨酸霉菌MSL 2211（KCTC 8926p）的特征在于表现出高可可替拉宁的高生产率。 活化肌动蛋白霉菌MSL2211的培养基的特征在于包含75-85重量％的蔗糖，10-20重量％的酵母提取物，2-5重量％的氨基酸，2.2-5重量％的无机盐 。 糖蔗糖的特征在于包含甘露糖。 氨基酸的特征在于包含天冬酰胺。 无机盐的特征在于包含2-4重量％的硫酸镁，0.1-0.5重量％的氯化钠，0.1-0.5重量％的氯化钙。