아민 카르바밀레이티드 유도체화와액체크로마토그래피/전기분무-이중질량분석기를 이용한인체의 뇨 또는 혈청에서의 폴리아민 검출 방법
    1.
    发明公开
    아민 카르바밀레이티드 유도체화와액체크로마토그래피/전기분무-이중질량분석기를 이용한인체의 뇨 또는 혈청에서의 폴리아민 검출 방법 无效
    使用液相色谱/电子离子化分光光度法分析尿素或等离子体中的聚氨酯的方法与氨基羰基化衍生物

    公开(公告)号:KR1020090027896A

    公开(公告)日:2009-03-18

    申请号:KR1020070093075

    申请日:2007-09-13

    Abstract: A method for detecting polyamine in urine or serum sample is provided to analyze it using sample of amount less than those of an existing method, to improve sensitivity of the analysis, to detect 9~10 kind of polyamines including cetyl polyamine at the same time and to decrease time, cost and effort required for analysis. A method for detecting polyamine in urine or serum sample includes steps of: derivatizing a polyamine within the urine or the serum sample into amine carbamylated and extracting selectively polyamine from the urine or the serum sample; separating the extracted polyamine using a high performance liquid chromatography(HPLC); and determining concentration of the polyamine by analyzing the separated polyamine by a mass spectrograph.

    Abstract translation: 提供了一种检测尿液或血清样品中多胺的方法,使用少于现有方法的样品进行分析,提高分析的灵敏度,同时检测9〜10种多胺,包括十六烷基多胺, 以减少分析所需的时间,成本和努力。 用于检测尿液或血清样品中多胺的方法包括以下步骤:将尿液或血清样品中的多胺衍生为氨基甲酰化胺,并从尿液或血清样品中选择性提取多胺; 使用高效液相色谱(HPLC)分离提取的多胺; 并通过质谱仪分析分离的多胺来测定多胺的浓度。

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