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    아스퍼질러스 니가에서 분리한 새로운 항진균성 단백질
    2.
    发明公开
    아스퍼질러스 니가에서 분리한 새로운 항진균성 단백질 失效
    从ASPERGILLUS NIGER分离的新型抗体蛋白

    公开(公告)号：KR1020000061133A

    公开(公告)日：2000-10-16

    申请号：KR1019990009957

    申请日：1999-03-24

    Applicant: 한국과학기술연구원

    Inventor： 이동건 ,  함경수 ,  김길룡 ,  맹철영 ,  신송엽 ,  서무열

    IPC: C07K14/195 ,  C07K14/38

    CPC classification number: C07K14/38 ,  A61K38/164

    Abstract: PURPOSE: Provided is a novel antimycotic protein isolated from the medium of Aspergillus niger which is harmless to a human body and mass cultured. The protein is excellent in antimycotic activity against variable pathogenic fungi therefore, it can be used for prevention and treatment of mycotic diseases. CONSTITUTION: A novel antimycotic protein consists of 58 amino acids represented by sequence No. 1 and its molecular weight is 6.2-7.0 kDa. The antimycotic protein shows antimycotic activity against pathogenic fungi such as Aspergillus fumigatus, Apergillus flavus, Candida albicans, Trichosporon beigelii and Saccaromyces cerevisiae and vegetable pathogenic fungi such as Fusarium oxysporum, Fusarium solani, and Aspergillus niger. The novel antimycotic protein is prepared by following steps: (a)inoculating a spore of Aspergillus niger to YPD medium containing 2% of dextrose, 1% of peptone, 0.5% of yeast extract; (b)performing shaking culture at 20 to 40°C for three to five days; (c)performing Amicon ultrafiltration of supernatant of culture after removing cell line; (d)separating supernatant of culture by ion-exchange filter using CM-sepharose ion exchange column; and (e)purifying the protein.

    Abstract translation: 目的：提供从黑曲霉培养基中分离的新型抗真菌蛋白，对人体无害，大量培养。 因此，蛋白质对可变致病真菌的抗真菌活性优异，可用于预防和治疗真菌病。 构成：新型抗真菌蛋白由序列号1所示的58个氨基酸组成，分子量为6.2-7.0kDa。 抗真菌蛋白质显示出对致病性真菌的抗真菌活性，例如烟曲霉，黄曲霉，白色念珠菌，白头翁和酿酒酵母，以及植物致病真菌如尖孢镰刀菌，茄病黑曲霉和黑曲霉。 通过以下步骤制备新型抗真菌蛋白质：（a）将黑曲霉孢子接种到含有2％葡萄糖，1％蛋白胨，0.5％酵母提取物的YPD培养基中; （b）在20〜40℃下进行振荡培养3〜5天; （c）除去细胞系后进行培养上清的Amicon超滤; （d）使用CM-sepharose离子交换柱通过离子交换过滤器分离培养上清; 和（e）纯化蛋白质。

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