公开(公告)号：KR1019880002999A

公开(公告)日：1988-05-13

申请号：KR1019860007182

申请日：1986-08-28

Applicant: 한국과학기술연구원

Inventor： 김정희 ,  오덕근 ,  박영훈

IPC: C12N11/14

Abstract: 내용 없음

公开(公告)号：KR100028422B1

公开(公告)日：1989-07-13

申请号：KR1019860007182

申请日：1986-08-28

Applicant: 한국과학기술연구원

Inventor： 김정희 ,  오덕근 ,  박영훈

IPC: C12N11/14

公开(公告)号：KR1019890001129B1

公开(公告)日：1989-04-24

申请号：KR1019860007182

申请日：1986-08-28

Applicant: 한국과학기술연구원

Inventor： 김정희 ,  오덕근 ,  박영훈

IPC: C12N11/14

Abstract: Penicillin was prepd. by the fermn. of Penicillium chrysogenum adsorbed on a porous matrix which supports the germination and growth of the spores. Thus, Penicillium spores were adsorbed on porous celite (porosity, 200-600 μm). The immobilized spores were cultivated on a fluidized reactor by recycling the medium contg. 1-2 lactose, 2 (SL, 0.5-1 (NH4)2SO4, 0.1-0.3 KH2PO4, 0.025 MgSO4. 7H2O, 2 mineral soln., 0.05 phenylacetic acid and 0.025% polypropylene glycol at the flow rate of 10-20 ml/hr at pH 6-8, 25-30≦̸C, 1-10 vvm and 10- 100% dissolved oxygen concentran. The recycled fed-batch fermn. which limit the phosphate concn. at 0.03-0.2 mg/ g/cell.hr increased the penicillin productivity.

Abstract translation: 青霉素已经制备好了 由fermn。 的产黄青霉吸附在支持孢子发芽和生长的多孔基质上。 因此，青霉孢子吸附在多孔硅藻土上（孔隙率为200-600μm）。 将固定化孢子在流化反应器中通过再循环培养基进行培养。 1-2乳糖，2（SL，0.5-1（NH4）2SO4，0.1-0.3KH 2 PO 4，0.025 MgSO 4·7H 2 O，2种矿物溶胶，0.05-苯乙酸和0.025％聚丙二醇，流速为10-20ml / 小时在pH 6-8,25-30＆amp; C，1-10vvm和10-100％溶解的氧浓度，再循环补料分批，限制磷酸盐浓度为0.03-0.2mg / g / cell.hr 提高青霉素的生产力。